ECOLOGY AND THE RAPD TECHNIQUES USED TO ASSESS THE GENETIC DIVERSITY IN PTEROLOBIUM HEXAPETALUM, A SCRAMBLING MEDICINAL SHRUB IN MARUTHAMALAI AND CHENNIMALAI HILLS, THE WESTERN GHATS

  • SHARMILA S. PG and Research Department of Botany, Vellalar College for Women (Autonomous), Thindal, Erode 638012, Tamil Nadu, India
  • AKILANDESWARI D. PG and Research Department of Botany, Vellalar College for Women (Autonomous), Thindal, Erode 638012, Tamil Nadu, India
  • RAMYA E. K. PG and Research Department of Botany, Vellalar College for Women (Autonomous), Thindal, Erode 638012, Tamil Nadu, India
  • MOWNIKA S. PG and Research Department of Botany, Vellalar College for Women (Autonomous), Thindal, Erode 638012, Tamil Nadu, India

Abstract

Objective: To investigate the ecological and genetic diversity, climatic factors, edaphic factors morphological and reproductive characters and RAPD analysis of medicinal plant species Pterolobium hexapetalum in two hills viz., Maruthamalai (arid) and Chennimalai (very arid), which is located in Coimbatore and Erode districts, Tamil Nadu.


Methods: The present research was carried out by using a random amplified polymorphic DNA (RAPD) analysis was made to determine the genetic variation between the two populations of the medicinal shrub, Pterolobium hexapetalum in an environmental gradient. Among the five primers tested, the OPN7 (80 %) and OPN17 (71.4 %) produced higher polymorphism was used in RAPD analysis.


Results: The results of RAPD analysis showed the presence of 51 individual bands were formed, out of which, 29 were polymorphic bands which showed the existence of genetic variation between populations. A dendrogram was constructed based on Jaccard’s coefficient to determine the degree of genetic relationship among the two populations and analysed. The primers OPN7 and OPN17 were clustered together at a genetic distance level 10. Considering the elevation and proximity, the temperature ranges from 18 °C to 37.6 °C in Maruthamalai hill and 20 °C to 39.4 °C in Chennimalai hill.


Conclusion: From the morphoecological studies the results indicated that both arid and very arid climatic conditions showed slight differences in their vegetative and reproductive characters.

Keywords: Genetic diversity, RAPD, Pterolobium hexapetalum, Jaccard’s coefficient

Downloads

Download data is not yet available.

Author Biography

SHARMILA S., PG and Research Department of Botany, Vellalar College for Women (Autonomous), Thindal, Erode 638012, Tamil Nadu, India

Department of Botany

References

1. FES. Assessment of biodiversity in sitamata wildlife sanctuary a conservation perspective. Report of Foundation for Ecological Security, Gujarat, India; 2010.
2. Kirtikar KR, Basu BD. Indian medicinal plants. 2nd edn. Vol 2. Bishen Singh Mahendra Pal Singh, Dehradun, India, International book distributor; 1975. p. 894-5.
3. Suresh D, Paulsamy S. Phenological observation and population dynamics of six uncommon medicinal plants in the grasslands of Nilgiris, Western Ghats, India. Maejo Int J Sci Technol 2010;4:185-92.
4. Fukuoka M, Niitani H, Suzuki A, Motomiya M, Hasegawa K, Nishiwaki Y, Kuriyama T, et al. A phase II study of CPT-11, a new derivative of camptothecin, for previously untreated non-small-cell lung cancer. J Clin Oncol 1992;10:16–20.
5. Qian Q, Hong C, Zongxiu S, Lihuang Z. The study on determining true and false hybrid rice; 1996.
6. Cao D, Oard JH. Pedigree and RAPD based DNA analysis of commercial U. S rice cultivars. Crop Sci 1997;37:1630-5.
7. Piper CS. Soil and plant analysis. Hans Publisher, Bombay; 1966.
8. Subbaiah BV, Asija GL. A rapid procedure for the estimation of available nitrogen in soil. Curr Sci 1956;25:259.
9. Olsen S, Cole C, Watanabe F, Dean L. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. USDA Circular Nr 939, US Gov. Print. Office, Washington, D. C; 1954.
10. Jackson ML. Soil chemical analysis. Prentice Hall of India Pvt. Ltd, New Delhi; 1973. p. 38-56.
11. Lindsay WL, Norvell WA. Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Sci Soc A J 1978;42:421-8.
12. Carlson JE, Tulsieram LK, Glaubitz JC, Luk VWK, Kauffeldt C, Rutledge R. Segregation of random amplified DNA markers in FI progeny of conifers. Theor Appl Genet 1991;83:194-200.
13. Nei N, Li W. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 1979;76:5269-73.
14. Rolf FJ. NTSYS-PC numerical taxonomy and multivariate system, Version 1.80; 1993.
15. Kunwar RM, Bussmann RW. Ethanobotany in the Nepal Himalaya. J Ethnobiol Ethnomed 2008;4:11.
16. Sarvalingam A, Rajendran A, Sivalingam R. Wild edible plant resources used by irulas of the maruthamalai hills. Southern Western Ghats, Coimbatore, Tamilnadu. Indian J Nat Prod Resour 2014;5:198-201.
17. Ganesh P, Nagendrababu G, Sudarsanam G. Pharmacognostical and analytical studies of leaves of pterolobium hexapetalum (Roth) santapau and wagh. J Pharm Res 2014;8:541-7.
18. Sindhu S, Uma G, Kumudha P. Survey of medicinal plants in Chennimallai Hills, Erode Districts, Tamilnadu. Asian J Plant Sci Res 2012;2:712-7.
19. Anjanadevi N. Survey of sporadic medicinal plants in Kanjamalai, Salem District IOSR J Human Soc (IOSR-JHSS); 2015. p. 92-4.
20. Parisi AF, BL Vallee. Zinc metalloenzymes: characteristics and significance in biology and medicine. Am J Clin Nutr 1969;22:1222-39.
21. Beulah Jerlin, Sharmila S, Kathiresan K, Kayalvizhi K. Zinc solubilizing bacteria from rhizospheric soil of mangroves. Int J Microbiol Biotechnol 2017;2:148-55.
22. Lewontin RC. The genetic basis of evolutionary change. Columbia University Press: New York, USA; 1974.
23. Patrica M Hogbin, Ayre DJ, Whelan RJ. Genetic variation and reproductive success of road verge populations of the rare shrub Grevillea barklyana (Proteaceae). J Hered 1998;80:180-6.
24. Chandler JW, Bartels D. Drought: avoidance and adaptation. In: Encyclopedia of Water Science. 2nd Edition. SW Trimble, BA Stewart, TA Howell. Taylor and Francis Group, London; 2008. p. 224.
25. Singh A, Dwivedi S, Bharti S, Srivastava A, Singh V, Khanuja S. Phylogenetic relationships as in Ocimum revealed by RAPD markers. Euphytica 2004;136:11-20.
26. De Masi L, Siviero P, Esposito C, Castaldo D, Siano F, Laratta B. Assessment of agronomic, chemical and genetic variability in common Basil (Ocimum basilicum L.). Eur Food Res Technol 2006;223:273–8.
27. Sathishkumar P, Paulsamy S, Anandakumar AM. Variation in physical characters and evaluation of genetic diversity in Acacia caesia var. caesia (L.) Willd. in an environmental gradient in the Western Ghats of Tamil Nadu, India. Maejo Int J Sci Technol 2010;4:533-9.
28. Gurudeepan S, Ramanathan T, Satyavani K, Dhinesh T. Standardisation of DNA isolation and PCR protocol for RAPD analysis of Suaeda sp. Asian J Biotechnol 2011;3:486-92.
Statistics
79 Views | 166 Downloads
Citatons
How to Cite
S., S., A. D., R. E. K., and M. S. “ECOLOGY AND THE RAPD TECHNIQUES USED TO ASSESS THE GENETIC DIVERSITY IN PTEROLOBIUM HEXAPETALUM, A SCRAMBLING MEDICINAL SHRUB IN MARUTHAMALAI AND CHENNIMALAI HILLS, THE WESTERN GHATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 3, Jan. 2019, pp. 72-77, doi:10.22159/ijpps.2019v11i3.30983.
Section
Original Article(s)