PHYTOCHEMICAL IDENTIFICATION AND ANTIOXIDANT ACTIVITY OF PASSIFLORA FOETIDA FRUITS AND LEAVES EXTRACTS: A COMPARATIVE STUDY
Objective: The objective of this study was to compare the phytochemical composition and antioxidant activity of Passiflora foetida fruits and leaves extract.
Methods: The parameters observed in this study were phytochemical compounds including alkaloid, flavonoid, phenolic, sterol, triterpenoid, saponin, tannin, and cardiac glycoside, total phenolic content Folin Ciocalteu method is based on reduction of Folin Ciocalteu reagent in alkaline medium; the metal complex produced measured at λmax: 760 nm; total flavonoids content with AlCl3 Colorimetric method based on complex formation of AlCl3 and flavonoid content in alkaline medium, the AlCl3-flavonoid complex produced measured at λmax: 510 nm; free radical DPPH scavenging activity; and ferric reducing power based on reduction of Fe3+ion into Fe2+ion that reacted with FeCl3 to form a ferric-ferrous complex that measured at λmax: 700 nm.
Results: Passiflora leaves extract has phytochemical compound such as alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides, total phenol was 22.92±0.18 mg GAE/g sample dry base, total flavonoid was 7.01±0.10 mg CE/g sample dry base, DPPH scavenging activity was 2.77±0.02 mg GAE/g sample dry base and ferric reducing power was 3.20±0.04 mg GAE/g sample dry base meanwhile Passiflora fruits extract had phytochemical compounds such as alkaloid, phenolic, flavonoids, cardiac glycosides, total phenol was 6.53±1.02 mg GAE/g sample dry base, total flavonoids were 1.56±0.27 mg CE/g sample dry base, DPPH free radical scavenging activity was 1.00±0.15 mg GAE/g sample dry base, and ferric reducing power was 1.12±0.17 mg GAE/g sample dry base.
Conclusion: Passiflora leaves extract has higher total phenol, total flavonoid and antioxidant activity measured by DPPH scavenging activity and ferric reducing power value compared with Passiflora fruits extract.
2. Quattrocchi U. CRC world dictionary of medicinal and poisonous plants common names, scientific names, eponyms, synonyms and, etymology; 2012. p. 2803-4.
3. Widyawati PS, TDW Budianta, FA Kusuma, dan EL Wijaya. Difference of solvent polarity to phytochemical content and antioxidant activity of pluchea indicia less leaves extracts. Int J Pharmacogn Phytochem Res 2014;6:850-5.
4. Sudarmadji S. Prosedur analisa untuk bahan makanan dan pertanian; 2007. p. 67-9, 83, 99-100.
5. Harborne JB. Phytochemical methods. Chapman and Hall, London; 1973. p. 14-20.
6. Muntana N, S Prasong. Study on total phenolic contents and their antioxidant activities of thai white, red, and black rice bran extracts. Pak J Biol Sci 2010;13:170-4.
7. Kumar S, D Kumar, Manjusha, K Saroha, N Singh, B Vashishta. Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) schrad methanolic fruit extract. Acta Pharm 2008;58:215-20.
8. Sompong R, S Siebenhandl-Ehn, G Linsberger Martin, E Berghofer. Physicochemical and antioxidative properties of red and black rice varieties from Thailand, China and Sri Lanka. Food Chem 2011;124:132-40.
9. Park YS, SJ Kim, HI Chang. Isolation of anthocyanins from black rice (Heugjinjubyeo) and screening of its antioxidant activities. J Microbial Biotechnol J Microb Biotechnol 2008;36:55-60.
10. Patterson GW, WD Nes. Physiology and biochemistry of sterols; 1991.
11. George M. Qualitative and quantitative phytochemical analysis on the leaves and fruits of Passiflora foetida. Int J Pharm Sci Invent 2017;6:26-30.
12. Odewo SA, AO Agbeja, KA Olaifa, AP Ojo, SA Ogundana. Proximate and spectroscopic analysis of Passiflora Foetida L. Int J Sci Technol Res 2014;3:353-6.
13. Decoteau DR. Principles of plant science environmental factors and technology in growing plants; 2005. p. 89.
14. Cheynier V, G Comte, KM Davies, V Lattanzio, S Martens. Plant phenolics: recent advances on their biosynthesis, genetics, and ecophysiology. Plant Physiol Biochem 2013;72:1-20.
15. Widyawati PS, CH Wijaya, PS Hardjosworo, dan D Sajuthi. Pengaruh ekstraksi dan fraksinasi terhadap kemampuan menangkap radikal bebas DPPH (1,1-difenil-2-pikrilhidrazil) ekstrak dan fraksi daun beluntas (Pluchea indica Less). Seminar Rekayasa Kimia dan Proses; 2010. p. 1-7.
16. Ramawat KG, JM Merillon. Natural products phytochemistry, botany and metabolism of alkaloids, phenolics, and terpenes. Berlin: Springer; 2013.
17. Robinson R. Ed. Plant Sciences. New York: Macmillan Reference USA; 2001. p. 156-7.
18. Ainsworth EA, KM Gillespie. Estimation of total phenolic content and other oxidation substrates in plant tissues using a folin-ciocalteu reagent. Nat Protoc 2007;2:875-7.
19. Anesini C, GE Ferraro, R Filip. Total polyphenol content and antioxidant capacity of commercially available tea (Camellia sinensis) in Argentina. J Agric Food Chem 2008;56:9225-9.
20. Rorong JA, E Suryanto. Analisis fitokimia eceng gondok (Eichhornia crassipes) dan efeknya sebagai agen photoreduksi Fe+3. J Chem Prog 2010;3:33-41.
21. Widyawati PS, YDW Werdani, C Setiokusumo, A Kartikasari. In vitro antioxidant capacities and antidiabetic properties of pluchea leaves and green tea mixtures at various proportions. Int J Pharm Pharm Sci 2017;9:203-8.
22. Aksoy L, E Kolay, Y Agilonu, Z Aslan, M Kargioglu. Free radical scavenging activity, total phenolic content, total antioxidant status, and total oxidant status of endemic Thermopsis turcica. Saudi J Biol Sci 2013;20:235-9.
23. Widyawati PS. Determination of antioxidant capacity in Pluchea Indica less leaves extract and its fractions. Int J Pharm Pharm Sci 2016;8:32-6.
24. Pokorny J, N Yanishlieva, M Gordon. Antioxidants in food practical applications; 2001. p. 10-7, 30-2.
25. Liu J, Wang C, Wang Z, Zhang C, Lu S, Liu J. The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides. Int Food Chem 2011;126:261–9.
This work is licensed under a Creative Commons Attribution 4.0 International License.