• Dina M. Hashim Pharmaceutics Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt,
  • Nermin M. Sheta Pharmaceutics Department, Faculty of Pharmacy, October 6 University, Giza, Egypt,
  • Vivian S. Elwazzan Pharmaceutics Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt,
  • Wedad Sakran Pharmaceutics Department, Faculty of Pharmacy, Helwan University, Ein Helwan, Egypt


Objective: Bemotrizinol (BEMT) is the most efficient broad-spectrum UV-absorber having a dual mechanism of action in absorbing and reflecting photons. The main objective of this work was to develop successful oil in water (o/w) nanoemulsion for improving the solubility of BEMT and its protective characteristics.

Methods: Pseudo-ternary phase diagrams were constructed using labrafac PG and isopropyl myristate as oil phase, tween 80 as surfactant (S) and cremophor EL as cosurfactant (CoS) the ratio of S/CoS was determined according to highest percent of water incorporation to the system. Full factorial study design (24) using Design-Expert® software was adopted to study the effect of four independent variables namely: oil type, oil concentration, S/CoSmix (3:1) concentrations and BEMT concentration on the particle size and the in vitro release at 2 h (Q2h) of the prepared nanoemulsion formulae. Two systems each of eight formulae were developed and evaluated through droplet size analysis, zeta potential measurement, refractive index, in vitro drug release and according to the desirability value two formulae (F6 and F14) were used for further evaluations including in vitro sun protection factor (SPF), ex-vivo deposition by tape stripping technique, permeation test and photostability study.

Results: Formula (F14) was chosen as the optimum formula having an in vitro SPF of 16.08±0.39, lowest permeation of 140±0.06 μg/cm2after six h and highest photostability (t90% = 168.02) after 120 min.

Conclusion: Despite the poor solubility of bemotrizinol, it could be enhanced by novel drug delivery systems with good SPF value while maintaining its photostability.

Keywords: Bemotrizinol, Nanoemulsion, In-vitro SPF, Ex-vivo deposition, Tape stripping technique, Photostability


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1. Haluza D, Simic S, Moshammer H. Sun exposure prevalence and associated skin health habits: results from the austrian population based uv skin risk survey. Int J Environ Res Public Health 2016;13:141.
2. Godwin D, Kim N, Felton L. Influence of transcutol CG on the skin accumulation and transdermal permeation of ultraviolet absorbers. Eur J Pharm Biopharm 2002;53:23-7.
3. Jimenez M, Pelletier J, Bobin M, Martini M. Influence of encapsulation on the in vitro percutaneous absorption of octyl methoxycinnamate. Int J Pharm 2004;272:45-55.
4. Sambandan D, Ratner D. Sunscreens: an overview and update. J Am Acad Dermatol 2011;64:748-58.
5. Latha M, Martis J, Shobha V, Sham R, Bangera S, Krishnankutty B, et al. A review sunscreening agents. Clin Aesthet Dermatol J 2013;6:16-26.
6. Puglia C, Damiani E, Offerta A, Rizza L, Tirendi G, Tarico S, et al. Evaluation of nanostructured lipid carriers (NLC) and nanoemulsions as carriers for uv-filters: characterization, in vitro penetration and photostability studies. Eur J Pharm Sci 2014;51:211-7.
7. Shaath NA. SPF boosters and photostability of ultraviolet filters antioxidants and other cosmetics ingredients to improve the uv filters and boost the SPF. HAPPI; 2007. p. 77-83.
8. Zhang Y, Shang Z, Gao C, Du M, Xu S, Song H, et al. Nanoemulsion for solubilization, stabilization and in vitro release of pterostilbene for oral delivery. AAPS PharmSciTech 2014;15:1000-8.
9. Devarajan V, Ravichandran V. Nanoemulsions: as modified drug delivery tool. pharmacie globale. Int J Comprehensive Pharmacy 2011;4:1-6.
10. Badawi A, Abd El-Aziz N, Amin M, Sheta N. Topical benzophenone-3 microemulsion based gels: preparation, evaluation and determination of microbiological uv blocking activity. Int J Pharm Pharm Sci 2014;6:562-70.
11. Nornoo A, Osborne D, Chow D. Cremophore free intravenous microemulsions for paclitaxel. formulation, cytotoxicity and hemolysis. Int J Pharm 2008;349:108-16.
12. Ali H, Hussein A. Oral nanoemulasion of candesartan cilexetil: formulation, characterization and in vitro drug release studies. AAPS PharmSciTech 2017;3:1-16.
13. Yadav S, Poddar S. Formulation, in vitro and in vivo evaluation of nanoemulsion gel for transdermal drug delivery of nimodipine. Asian J Pharm Clin Res 2015;8:119-24.
14. Marques M, Loebenberg R, Almukainzi M. Simulated biological fluids with possible application in dissolution testing. Dissolution Technologies 2011;15-28. dx.doi.org/10.14227/ DT180311P15.
15. Kumar S, Aparna C, Raju B. Formulation and evaluation of losartan potassium nanoemulsion gel. Eur J Biomed Pharm Sci 2018;5:579-89.
16. Azeem A, Rizwan M, Ahmed F, Iqbal Z, Khar R, Aqil M, et al. Nanoemulsion components screening and selection: a technical note. AAPS PharmSciTech 2009;10:69-76.
17. Pathan I, Setty C. Stability evaluation of tamoxifen citrate nanoemulsion containing cremophor RH40 as surfactant. Acta Pharm Sci 2011;53:127-34.
18. Zhao Y, Wang C, Chow AH, Ren K, Gong T, Zhang Z, et al. Self-nanoemulsifying drug delivery system (SNEDDS) for oral delivery of zedoary essential oil: formulation and bioavailability studies. Int J Pharm 2010;383:170-7.
19. Sahoo S, Panyam J, Prabha S, Labhasetwar V. Residual polyvinyl alcohol associated with poly (D, L-lactide-coglycolide) nanoparticles affects their physical properties and cellular uptake. J Controlled Release 2002;82:105-14.
20. Alsofany J, Hamza M, Abdelbarry AA. Fabrication of nanosuspension directly loaded fast-dissolving films for enhanced oral bioavailability of olmesartan medoxomil: in vitro characterization and pharmacokinetic evaluation in healthy human volunteers. AAPS PharmSciTech 2018;19:2118-32.
21. Donglikar M, Deore S. A review article: sunscreens. Pharmacogn J 2016;8:171-9.
22. Pissavini M, Alard V, Heinrich U, Jenni K. In vitro assessment of water resistance of sun care products: a reproducible and optimized in vitro test method. Int J Cosmet Sci 2007; 29:451-60.
23. Stokes R, Diffey BL. In vitro assessment of sunscreen photostability: the effect of radiation source, sunscreen application thickness and substrate. Int J Cosmet Sci 1999;21:341-51.
24. Monteiro M, Ozzetti R, Vergnanini A, Gitirana L, Volpato N, Freitas Z, et al. Evaluation of octyl p-methoxycinnamate included in liposomes and cyclodextrins in anti-solar preparations: preparations, characterizations and in vitro penetration studies. Int J Nanomed 2012;7:3045-58.
25. Stephane P. Stability of cosmetic formulations containing UV filters and preservatives, based on physical and chemical parameters. MOJ Toxicol 2015;1:12-21.
26. Ruela A, Perissinato A, Lino M, Mudrik P, Pereira G. Evaluation of skin absorption of drugs from topical and transdermal formulation. Braz J Pharmsci 2016;52:527-44.
27. Baboota S, Shakeel F, Ahuja A, Ali J, Shafig S. Design, development and evaluation of noval nanoemulsion formulations for transdermal potential of celecoxib. Acta Pharm 2007;57:315-32.
28. Junyaprasert V, Teeranachaideekul V, Souto EB, Boonne P, Muller RH. Q10 loaded NLC versus nanoemulsions: Stability, rheology and in vitro skin permeation. Int J Pharm 2009;377:207-14.
29. Bsieso E, Nasr M, Sammour O, Abd EL, Gawad N. Could nanovesicles containing a penetration enhancer clinically improve the therapeutic outcome in skin fungal diseases? Nanomed J 2015;10:2017-31.
30. Garoli D, Pelizzo M, Bernardini B, Nicolosi P. Sunscreen tests: correspondence between in vitro data and values reported by the manufacturers. J Dermatol Sci 2008;52:193-204.
31. Hojerova J, Medovcikova A, Mikula M. Photoprotective efficacy and photostability of fifteen sunscreen products having the same label SPF subjected to natural sunlight. Int J Pharm 2011;408:27-38.
32. Kockler J, Oelgemöller M, Robertson S, Glass B. Photostability of sunscreens. J Photochem Photobiol C 2012;13:91-110.
33. Badawi A, Abd El-Aziz N, Amin M, Sheta N. Almond oil based oleogels as cosmetic bases for liposoluble sun-screening agent bemotrizinol: in vitro characterization and photostability. Inventi Rapid Cosmeceuticals 2015;4:175-85.
34. Gonzalez H, Tarras Wahlberg N, Stromdahl B, Juzeniene A, Moan J, Larko O, et al. Photostability of commerical sunscreens upon sun exposure and irradiation by ultraviolrt lamps. BMC Dermatol 2007;7:1-9.
35. Chen H, Chang X, Weng T, Zhaox X, Gao Z, Yang Y, et al. A study of microemulsion systems for transdermal delivery of triptolide. J Controlled Release 2004;98:427-36.
36. Mahesh B, Vasanth K, Gowda D, Srivastava A, Raghundan H, Shinde C, et al. Enhanced permeability of cyclosporine from a transdermally applied nanoemulgel. Der Pharm Sin 2015;6:69-79.
37. Archita P, Anita L. Self microemulsifying drug delivery system as a potential drug delivery system for protease inhibitors in the treatment of AIDS. Asian J Pharm Sci 2011;6:226-40.
38. Elmataeeshy M, Sokar M, Baheyeldin M, Shaker D. Enhanced transdermal permeability of terbinafine through novel nanoemulgel formulation, development, in vitro and in vivo characterization. Future J Pharm Sci 2018;4:18-28.
39. Moghimipour E, Salimi A, Karami M, Isazadeh S. Preparation and characterization of dexamethasone microemulsion based on pseudoternary phase diagram. Jundishapur G Nat Pharm Prod 2013;8:105-12.
40. Sakeena M, Muthanna F, Abdullah G, Kanakal M. Formulation and in vitro evaluation of ketoprofen in palm oil esters nanoemulsion for topical delivery. J Oleo Sci 2010;59:223-8.
41. Nemichand S, Laxman S. Solubility enhancement of nebivolol by microemulsion technique. J Young Pharm 2016;8:356-67.
42. Illing A, Unruh T. Investigation on the flow behavior of dispersion of solid triglyceride nanoparticles. Int J Pharm 2004;284:123-31.
43. Figueiredo K, Neves J, Silva J, Freitas R. Phenobarbital loaded microemulsion: development, kinetic release and quality control. Braz J Pharm Sci 2016;2:251-63.
44. Mortazavi S, Pishrochi S, Jafari AZ. Formulation and in vitro evaluation of tretinooin microemulsion as a potential carrier for dermal drug delivery. IJJPR 2013;12:599-609.
45. Montenegro L, Carbone C, Puglisi G. Vehicle effects on in vitro release and skin permeation of octyl-methoxycinnamate from microemulsions. Int J Pharm 2011;405:162-8.
46. Shalviri A, Sharma A, Patel D, Sayani A. Low surfactant microemulsions for enhanced topical delivery of poorly soluble drugs. J Pharm Pharm Sci 2011;14:315-24.
47. Ruszkiewicz J, Pinkas A, Ferrer B, Peres TV, Tsatsakis A, Aschner M. Neurotoxic effect of active ingredients in sunscreen products, a contemporary review. Toxicol Rep 2017;4:245-59.
48. Varvaresou A. Percutaneous absorption of organic sunscreens. J Cosmet Dermatol 2006;5:53-7.
49. Tampucci S, Burgalassi S, Chetoni P, Monti D. Cutaneous permeation and penetration of sunscreens: formulation strategies and in vitro methods. Cosmetics J 2018;5:1-17.
50. Khurana S, Jain NK, Bedi PM. Nanoemulsion based gel for transdermal delivery of meloxicam: physic-chemical, mechanistic investigation. Life Sci 2013;92:383-92.
51. Couteau C, Faure A, Fortin J, Paparis E, Coiffard LJ. Study of the photostability of 18 sunscreens in creams by measuring the SPF in vitro. J Pharm Biomed Anal 2007;44:270-3.
52. Kullavanijaya P, Lim H. Photoprotection. J Am Acad Dermatol 2005;52:937-58.
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How to Cite
Hashim, D. M., N. M. Sheta, V. S. Elwazzan, and W. Sakran. “ENHANCING THE SUNSCREEN EFFICACY OF BEMOTRIZINOL MICROPIGMENT BY USING O/W NANOEMULSION TOPICAL PREPARATIONS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 7, July 2019, pp. 47-56, doi:10.22159/ijpps.2019v11i7.32652.
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