CHARACTERIZATION OF THE MUCILAGES EXTRACTED FROM HIBISCUS ROSA-SINENSIS LINN AND HIBISCUS MUTABILIS LINN AND THEIR SKIN MOISTURIZING EFFECT


Waruttaya Kassakul, Werner Praznik, Helmut Viernstein, Darunee Hongwiset, Ampai Phrutivorapongkul, Pimporn Leelapornpisid

Abstract


Objective: The present study was aimed to investigate the physicochemical properties, chemical characterization and skin moisturizing effect of the mucilages extracted from Hibiscus rosa-sinensis (HR), and H. mutabilis (HM).

Methods: The HR and HM leaves were extracted with distilled water to obtain their mucilages. Then dried and powdered. The physicochemical properties of the mucilage powders, including pH value, swelling index, loss on drying, solubility, total ash, acid-insoluble ash and carbohydrate content were evaluated. The polysaccharides were isolated from the mucilages and purified by DEAE-650M column and identified the sugar unit constituents by acid hydrolysis, followed by TLC and HPAEC analyses. The short-term moisturizing effect of the mucilages was determined on pig skin using Corneometer®.

Results: The yield of HR and HM mucilages was 21% and 15% w/w. Each of mucilage showed the specific physicochemical properties. The main component of HR and HM mucilages was acidic polysaccharides named as AHR and AHM. The major components with the Mol% of AHR were 27% galactose, 24% rhamnose, 19% galacturonic acid, and 18% arabinose, while AHM were rich in 27% rhamnose, 25% galactose, 18% xylose, 16% arabinose and 9% galacturonic acid. The skin moisturizing effect of 0.2 % HR mucilage was significantly more effective than 0.2% HM mucilage, 0.2% hyaluronic acid, 5% propylene glycol and 5% butylene glycol at 30 min after application.

Conclusion: The results suggest that mucilage extracted from Hibiscus rosa-sinensis was more superior in quality than Hibiscus mutabilis mucilage for using as a good moisturizer in the skin care product.

 


Keywords


Hibiscus rosa-sinensis, Hibiscus mutabilis, Mucilage, acidic polysaccharide, Skin moisturizing effect.

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About this article

Title

CHARACTERIZATION OF THE MUCILAGES EXTRACTED FROM HIBISCUS ROSA-SINENSIS LINN AND HIBISCUS MUTABILIS LINN AND THEIR SKIN MOISTURIZING EFFECT

Keywords

Hibiscus rosa-sinensis, Hibiscus mutabilis, Mucilage, acidic polysaccharide, Skin moisturizing effect.

Date

04-11-2014

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 6, Issue 11, 2014 Page: 453-457

Online ISSN

0975-1491

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2012 Views | 174 Downloads

Authors & Affiliations

Waruttaya Kassakul
Chiang Mai University
Thailand

Werner Praznik
University of Vienna
Austria

Helmut Viernstein
University of Vienna
Austria

Darunee Hongwiset
Chiang Mai University
Thailand

Ampai Phrutivorapongkul
Chiang Mai University
Thailand

Pimporn Leelapornpisid
Chiang Mai University
Thailand


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