ANTIPROLIFERATIVE EFFECT OF FLOWER EXTRACTS OF SPILANTHES PANICULATA ON HEPATIC CARCINOMA CELLS
Keywords:
Spilanthes paniculata flowers, Antiproliferative effect, Huh-7 cells, Caspase-3, DNA transillumination, Tyrosine kinaseAbstract
Objective: The development and evaluation of new antiproliferative drugs obtained from natural resources has gained its importance because of their less cytotoxic properties. There is no such report regarding antiproliferative effect of Spilanthes paniculata Linn. flower. Therefore, the present study was undertaken to evaluate the antiproliferative effect of Spilanthes paniculata Linn. flower.
Methods: In order to achieve this goal, the dried flowers were extracted in petroleum ether, ethyl acetate and ethanol. All these three extracts of varying concentrations were subjected to further evaluation of antiproliferative action on human hepatoma cell line (Huh-7 cells). In order to understand the mechanism of antiproliferative effect of these three extracts, various studies like caspase-3 enzyme assay, DNA transillumination assay and receptor tyrosine kinase profiling were performed. Separately, we estimated total flavonoid and phenolic contents and in vitro free radical scavenging properties of these extracts.
Results: The results indicated that both ethyl acetate and ethanol extracts possessed antiproliferative effect on Huh-7 cells because of their induction of caspase-3 enzymes and inhibition of phosphorylation of various tyrosine kinases. It was observed that during the transillumination assay of the ethyl acetate and ethanol extracts, DNA of Huh-7 cells were also degraded. It was also found that these two extracts possessed potent antiproliferative effect on Huh-7 cells due to the presence of rich amount of phenols and flavonoids.
Conclusion: Based on our data, both ethyl acetate and ethanol extracts might be beneficial for the future development of antiproliferative therapeutics in drug design perspective.
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References
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