IMMUNOMODULATORY, ANTICANCER AND ANTIOXIDANT ACTIVITIES OF CYCLEA PELTATA (LAM.) HOOK. F. AND THOMSON
Objective: The present study was performed to evaluate the immunomodulatory, anticancer, and antioxidative properties of the fraction (CP_2) isolated from Cyclea peltata.
Methods: Immunomodulation was evaluated in lymphocytes by lymphocyte proliferation assay and in THP-1 macrophage cell lines by MTT assay. The nitrite production by the macrophages was also measured by the nitrite assay using griess reagent. The anticancer activity of the fraction was determined by MTT assay. The antioxidant activity was evaluated by DPPH assay and total antioxidant assay by phosphomolybdenum method. It is expressed as number of gram equivalent of ascorbic acid.
Results: The plant fraction showed the presence of flavonoids which induced lymphocyte proliferation rate of 4.29±0.007 at 100 μg/ml. It was not toxic to THP-1 macrophage cells and also could induce nitrite production at 1 mg/ml. It also exhibited good anticancer activity at 100 μg/ml after 48h of incubation. The DPPH activity was found to be low since 100 μg/ml showed only an inhibition rate of 22±0.026. The total antioxidant activity at 1000 μg/ml of CP_2 was found to be equivalent to 79±0.03 μg/ml of ascorbic acid exhibiting moderate antioxidant activity.
Conclusion: The fraction CP_2 containing flavonoid, isolated from Cyclea peltata has good immunomodulatory, antioxidant and anticancerous property.
2. Vaibhav D Aher, Arun Kumar Wahi. Immunomodulatory effect of alcoholic extract of Terminalia chebula ripe fruits. J Pharm Sci Res 2010;2 Suppl 9:539-44.
3. Dashputre NL, Naikwade NS. Immunomodulatory activity of Abutilon Indicum linn on albino mice. Int J Pharm Sci Res 2010;1 Suppl 3:178-84.
4. Xiaojuan He, Xuyan Niu, Jian Li, Shaohua Xu, Aiping L. Immunomodulatory activities of five clinically used Chinese herbal polysaccharides. J Exp Integ Med 2012;2 Suppl 1:15-27.
5. Vivek K Bajpai, Rajib Majumder, Jae Gyu Park. Isolation and purification of plant secondary metabolites using column-chromatographic technique. Bangladesh J Pharmacol 2016;11:844-8.
6. Rebecca L Siegel, Kimberly D Miller, Ahmedin Jemal. Cancer statistics. Cancer J Clin 2017;67Suppl 1:7-30.
7. N Oue. Gene expression profile of gastric carcinoma–identification of genes and tags potentially involved in invasion, metastasis and carcinogenesis by serial analysis of gene expression. Cancer Res 2004;64:2397-405.
8. Thangarajan Rajkumar, Neelakantan Vijayalakshmi, Gopisetty Gopal, Kesavan Sabitha, Sundersingh Shirley, Uthandaraman M Raja, et al. Identification and validation of genes involved in gastric tumorigenesis. Cancer Cell Int 2010;10 Suppl 45:10-45.
9. Katia C Carvalho, Isabela W Cunha, Rafael M Rocha, Fernanda R Ayala, Mariana M Cajaiba, Maria D Begnami, et al. GLUT1 expression in malignant tumors and its use as an immunodiagnostic marker. Clinics (Sao Paulo) 2011;66 Suppl 6:965–72.
10. JM Sasikumar, V Maheshu, G Smilin, Bell Aseervatham, D Teepica, Priya Darshini. In vitro antioxidant activity of Hedyotis corymbosa (L.) lam. aerial parts. Indian J Biochem Biophys 2010;47:49-52.
11. Jyothi Thomas, Meena Cheruvathur, Bince Mani, Dennis Thomas. A rapid in vitro multiplication system for commercial propagation of pharmaceutically important Cyclea peltata (Lam) Hook
13. Jyothi Abraham, T Dennis Thomas. Antibacterial activity of medicinal plant Cyclea peltata (Lam) hooks and thomas. Asian Pac J Trop Dis 2012;2 Suppl 1:280-4.
14. VJ Shine, GI Anuja, SR Suja, Gopan Raj, PG Latha. Bioassay guided fractionation of Cyclea peltata using in vitro RAW 264.7 cell culture, antioxidant assays and isolation of bioactive tetrandrine. J Ayurveda Integr Med 2018;9 Suppl 4:312-5.
15. A Poongothai, KP Sreena, K Sreejith, M Uthiralingam, S Annapoorani. Preliminary phytochemical screening of Ficus racemosa Linn. Int J Pharma Bio Sci 2011;2 Suppl 2:431-4.
16. Manu M Joseph, Aravind SR, Sheeja Varghese, Mini S, Sreelekha TT. Evaluation of antioxidant, antitumor and immunomodulatory properties of polysaccharide isolated from fruit rind of Punica granatum. Mol Med Rep 2012;5:489-96.
17. Dong Pil Won, Jong Seok Lee, Duck Soo Kwon, Keun Eok Lee, Won Cheol Shin, Eock Kee Hong. Immunostimulating activity by polysaccharides isolated from fruiting body of Inonotus obliquus. Mol Cells 2011;31:165-73.
18. Mahnaz Khanavi, Mana Moshteh, Azadeh Manayi, Mohammad Reza Shams Ardekan, Mahdi Vazirian, Yousef Ajani, et al. Cytotoxic activity of Lythrum salicaria L. Res J Biol Sci 2011;6 Suppl 2:55-7.
19. Villano D, Fernandez Pachon MS, Troncoso AM, Garcia Parrilla MC. Comparison of antioxidant activity of wine phenolic compounds and metabolites in vitro. Anal Chim Acta 2005;38:391-8.
20. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337–41.
21. Danny K Asami, Yun-Jeong Hong, Diane M Barrett, Alyson E Mitchell. Comparison of the total phenolic and ascorbic acid content of freeze-dried and air-dried marion berry, strawberry, and corn grown using conventional, organic, and sustainable agricultural practices. J Agric Food Chem 2003;51:1237-41.
22. F Pourmorad, SJ Hosseinimehr, N Shahabimajd. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotech 2006;5 Suppl 11:1142-5.
23. Kapil S Patil, Sanjivani R Bhalsing. Larvicidal activity of Cyclea peltata against Culex spp. Int J Res Biosci Agric Tech 2015; Suppl 6:53-6.
24. HE Barksby, CJ Nile, KM Jaedicke, JJ Taylor, PM Preshaw. Differential expression of immunoregulatory genes in monocytes in response to Porphyromonas gingivalis and Escherichia coli lipopolysaccharide. Clin Exp Immunol 2009;156:479–87.
25. NP Flavey, S Frankenburg. Appraisal of the total blood lymphocyte proliferation assay as a diagnostic tool in screening for tuberculosis. J Med Microbiol 1992;37:283-5.
26. Guiqing Wang, Mary M Petzke, Radha Iyer, Hongyan Wu, Ira Schwartz. Pattern of proinflammatory cytokine induction in RAW264.7 mouse macrophages is identical for virulent and attenuated Borrelia burgdorferi. J Immunol 2008;180 Suppl 12:8306-15.
27. Jin Dai, Russell J Mumper. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules 2010;15:7313-52.
28. Gordon M Cragg, David J Newman. Plants as a source of anti-cancer agents. J Ethnopharmacol 2005;100:72–9.
29. Felth J, Rickardson L, Rosen J, Wickstrom M, Fryknas M, Lindskog M, et al. Cytotoxic effects of cardiac glycosides in colon cancer cells, alone and in combination with standard chemotherapeutic drugs. J Nat Prod 2009;72:1969-74.
30. Nooman A Khalaf, Ashok K Shakya, Atif AL-Othman, Zaha El-Agbar, Husni Farah. Antioxidant activity of some common plants. Turk J Biol 2008;32:51-5.
31. Suresh Subramanian, Suriyavathana Vedanarayanan. In vitro antioxidant potential of ethanolic extract of Anisomeles Malabarica. Int Res J Pharm 2012;5:394-8.
This work is licensed under a Creative Commons Attribution 4.0 International License.