• ANKITA SHARMA Department of Pharmaceutical Chemistry, Abhilashi College of Pharmacy, Nerchwok, Mandi, HP, India
  • INDER KUMAR Department of Pharmaceutics, School of Pharmacy, Abhilashi University, Mandi, HP, India
  • KARAN RANA Quality Control Executive, Complex Pharma, Karnal, Haryana, India


Objective: The present study was undertaken to develop and validate an RP-HPLC method for the combination of imiquimod and salicylic acid

Methods: The method was carried out on Nucleodur C18 (250 mm × 4.6 mm I.D., 5 ????m) using low-pressure gradient elution mode. The mobile phase was used as 30M potassium dihydrogen phosphate and acetonitrile (45:55) pH 6.5 adjusted using ortho-phosphoric acid. The concentration of solvents was 1-20 µg/ml and the volume of injection was 20 mcl with the flow rate of 1.0 ml/min. The absorption maxima of salicylic acid and imiquimod were found 234 nm and 226 nm, respectively.

Results: The method was validated and showed the linearity greater than 0.99% and with precision (RSD%<1). The limit of detection (LOD) and limit of quantification (LOQ) of salicylic acid was found to be 0.09756 µg/ml and 0.2956 µg/ml, respectively, and imiquimod was found to be 0.044031 µg/ml and 0.13334 µg/ml, respectively.

Conclusion: The method developed in the present study was found to be sensitive, specific, and can be applied for the simultaneous estimation of imiquimod and salicylic acid.

Keywords: Imiquimod, Salicylic acid, Reverse-phase high-performance liquid chromatography (RP-HPLC), Validation, International council for harmonization (ICH)


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How to Cite
SHARMA, A., I. KUMAR, and K. RANA. “RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE COMBINATION OF IMIQUIMOD AND SALICYLIC ACID”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 9, Sept. 2020, pp. 41-48, doi:10.22159/ijpps.2020v12i9.38382.
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