FORTIFIED DADIH (FERMENTED BUFFALO MILK) WITH VITAMIN D3 IMPROVES INTERLEUKIN-6 AND CAECUM SHORT CHAIN FATTY ACIDS ON DIET-INDUCED OBESE RAT

  • Sari Bema Ramdika Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia https://orcid.org/0000-0001-9083-3740
  • Endang Mahati Department of Pharmacology and Therapy, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia http://orcid.org/0000-0002-9101-8753
  • Anang Mohamad Legowo Department of Food Technology, Faculty of Animal and Agricultural Sciences, Diponegoro University, Semarang 50275, Indonesia
  • Muflihatul Muniroh Department of Physiology, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia https://orcid.org/0000-0003-0020-6722
  • Yora Nindita Department of Pharmacology and Therapy, Faculty of Medicine, Diponegoro University, Semarang 50275, Indonesia http://orcid.org/0000-0003-1446-8141
  • Diana Nur Afifah Universitas Diponegoro

Abstract

Objective: This study aims to determine the effect of fortified dadih with vitamin D3 on IL-6 expression level and the concentration of caecum SCFA in obese rats.


Methods: A total of 30 male Sprague Dawley rats were divided into five equal groups: healthy-control-(K-), obese-control-(K+), obese-intervention-(X1, X2, and X3). K(+), X1, X2, and X3 were in obesity conditions which was induced by a high-fat sucrose diet (HFSD) and K(-) as a healthy-control-group. Furthermore, vitamin D3-fortified dadih at doses of 4 g/200 g-body-weight/d, dadih only at doses of 4 g/200 g-body-weight/d, and vitamin D3 only at 36 IU/200 g-body-weight/d was administered to X1, X2, and X3 groups, respectively.


Results: Treatment using fortified dadih with vitamin D3 showed significantly reduce weight gain (p<0.05) compare to K(+) and X2. In addition, X1 showed a decreased level of Interleukin-6 expression (p<0.05) than K(+), X2, and X3 groups but higher than K(-). Also, it showed the highest total SCFA, acetate, and propionate concentration (p<0.05). However, a moderately negative correlation was discovered between the pair of total SCFA and Interleukin-6 expression, acetate and Interleukin-6 expression, SCFA and body weight, propionate and body weight, butyrate, and body weight. On the contrary, a strong positive correlation was observed between the pair of Interleukin-6 expression levels and body weight.


Conclusion: This study shows that fortified dadih with vitamin D3 from fermented foods improve the expression level of Interleukin-6 and increase production of SCFA. Also, they improve instetinal hemeostatis because of the increased SCFA production.

Keywords: obesity, dadih, vitamin D3, IL-6, SCFA

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Ramdika, S. B., E. Mahati, A. M. Legowo, M. Muniroh, Y. Nindita, and D. N. Afifah. “FORTIFIED DADIH (FERMENTED BUFFALO MILK) WITH VITAMIN D3 IMPROVES INTERLEUKIN-6 AND CAECUM SHORT CHAIN FATTY ACIDS ON DIET-INDUCED OBESE RAT ”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 11, Sept. 2020, https://innovareacademics.in/journals/index.php/ijpps/article/view/39209.
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