HAIR GROWTH PROMOTING ACTIVITY OF CARTHAMUS TINCTORIUS FLORETS EXTRACT-LOADED NANOSTRUCTURED LIPID CARRIERS
Objectives: This work was aimed to formulate, characterize and determine hair growth promoting activity of the Carthamus tinctorius (safflower) florets extract-loaded nano structured lipid carriers (NLC) in C57BL/6 mice.
Methods: Safflower florets were extracted by maceration with ethanol, and then incorporated into NLC formula. Safflower extract-loaded NLCs were assessed for their physical properties, stabilities and hair growth promoting activity in C57BL/6 mice.
Results: Safflower-loaded NLCs had particle size around 100 nm, zeta potential in the range of -40 to -49 mV. The data from DSC and XRD suggested that this NLC occurred as an amorphous type NLC. Safflower extract-loaded NLC promoted hair growth in the mice better than minoxidil. Safflower yellow, the principle phytochemical in safflower extract, along with synergistic activity between other phytochemicals may account for hair growth promoting activity observed in mice. The further investigations in human volunteer should be conducted for the confirmation.
Conclusion: Safflower-loaded NLC that provided good physical properties and stabilities, exerted hair growth promoting activity in C57BL/6 mice.
2. Sinclair RD. Male androgenetic alopecia. J Men Health Gender 2004;1(4):319-27.
3. Lacy CF, Armstrong LL, Goldman MP, Lance LL. Drug information handbook with international trade names index, 17th ed. Ohio: Lexi Comp Inc; 2008. p. 652-3.
4. Traish AM, Mulgaonkar A, Giordano N. The dark side of 5Î±-reductase inhibitorsâ€™ therapy: sexual dysfunction, high gleason grade prostate cancer and depression. Korean J Urol 2014;55:367-79.
5. Abramowicz M. Propecia and rogain extra strength for alopecia. Med Lett Drugs Ther 1998;40:25-7.
6. Matsuda H, Yamazaki M, Matsuo K, Asanuma Y, Kubo M. Anti-androgenic activity of Myricae cortex-isolation of active constituents from bark of Myrica rubra. Biol Pharm Bull 2001;24:259â€“63.
7. Park W-S, Lee C-H, Lee B-G, Chang I-S. The extract of Thujae occidentalis semen inhibited 5Î±-reductase and adrochronogenetic alopecia of B6CBAF1/j hybrid mouse. J Dermatol Sci 2003;31:91â€“8.
8. Hirata N, Tokunaga M, Naruto S, Iinuma M, Matsuda H. Testosterone 5Î±-reductase inhibitory active constituents of Piper nigrum leaf. Biol Pharm Bull 2007;30:2402â€“5.
9. Murata K, Noguchi K, Kondo M, Onishi M, Watanabe N, Okamura K, et al. Promotion of hair growth by rosmarinus officinalis leaf extract. Phytother Res 2013;27:212-7.
10. Kwon OS, Han JH, Yoo HJ, Chung KH, Cho KH, Eun HC, et al. Human hair growth enhancement in vitro by green tea epigallocatechin-3-gallate. Phytomed 2007;14:551-5.
11. Tian Y, Yang Z-F, Li Y, Qiao Y, Yang J, Jia Y-Y, et al. Pharmacokinetic comparisons of hydroxysafflower yellow a in normal and blood stasis syndrome rats. J Ethnopharmacol 2010;129:1-4.
12. Kumar N, Rungseevijitprapa W, Narkkhong NA, Suttajit M, Chaiyasut C. 5Î±-reductase inhibition and hair growth promotion of some Thai plants traditionally used for hair treatment. J Ethnopharmacol 2012;139:765-71.
13. Junlatat J, Sripanidkulchai B. Hair growth-promoting effect of Carthamus tinctorius floret extract. Phytother Res 2014;28:1030-6.
14. Patzelt A, Knorr F, Blume-Peytavi U, Sterry W, Lademann J. Hair follicles, their disorders and their opportunities. Drug Discov Today Dis Mech 2008;5(2):e173-81.
15. Schaefer H, Lademann J. The role of follicular penetration. A differential view. Skin Pharmacol Appl 2001;14(S1):23-7.
16. MÃ¼ller RH, Lucks JS. Medication vehicles made of solid lipid particle (Solid lipid nanospheres SLN); 1996.
17. Wissing SA, MÃ¼ller RH. Cosmetic applications for solid lipid nanoparticles (SLN). Int J Pharm 2003;254:65-8.
18. Pardeike J, Hommoss A, MÃ¼ller RH. Lipid nanoparticles (SLN, NLC) in cosmetic and pharmaceutical dermal products. Int J Pharm 2009;366:170-84.
19. MÃ¼ller RH, Petersen RD, Hommoss A, Pardeike J. Nanostructured lipid carriers (NLC) in cosmetic dermal products. Adv Drug Deliv Rev 2007;59:522-30.
20. MÃ¼ller RH, Radtke M, Wissing SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. Adv Drug Deliv Rev 2002;54(S1):S131-5.
21. Mehnert W, MÃ¤der K. Solid lipid nanoparticles: production, characterization and applications. Adv Drug Deliv Rev 2001;47:165-96.
22. Mishra PR, Al Shaal L, MÃ¼ller RH, Keck CM. Production and characterization of hesperidin nanosuspensions for dermal delivery. Int J Pharm 2009;371:182-9.
23. Kumar N, Tharatha S, Chaiyasut C. Development and validation of simple isocratic high performance liquid chromatography-ultraviolet (HPLC-UV) method for determination of safflower yellow in Carthamus tinctorius L.-loaded nanostructured lipid carriers (NLC). Afr J Pharm Pharmacol 2011;5(20):2335-41.
24. Jenning V, ThÃ¼nemann AF, Gohla SH. Characterization of a novel solid lipid nanoparticle carrier system based on binary mixtures of liquid and solid lipids. Int J Pharm 2000;199:167-77.
25. Hu F-Q, Jiang S-P, Du Y-Z, Yuan H, Ye Y-Q, Zeng S. Preparation and characteristics of monostearin nanostructured lipid carriers. Int J Pharm 2006;314:83-9.
26. Slominski A, Paus R, Plonka P, Chakraborty A, Maurer M, Pruski D, et al. Melanogenesis during the anagenâ€“catagenâ€“telogen transformation of the murine hair cycle. J Invest Dermatol 1994;102:862â€“9.
27. Liang T, Liao S. Inhibition of steroid 5-alpha-reductase by specific aliphatic unsaturated fatty acids. Biochem J 1992;285:557-62.