EVALUATING THE CYTOTOXIC POTENTIAL OF METHONOLIC LEAF EXTRACT OF ALEO VERA ON MCF-7 BREAST CANCER CELL LINES

  • Renuka Srihari Maharani Lakshmi Ammanni College for Women
  • Austin Richard Surendranath University of Mysore
  • Nandashree Kasturacharya Maharani Lakshmi Ammanni College for Women
  • Karigar Chandrakanth Shivappa Tumkur University
  • Niranjali Devaraj Sivasitambaram University of Madras
  • Bhadrapura Lakkappa Dhananjaya Toxinology/Toxicology and Drug Discovery Unit, Centre for Emerging Technologies (CET), Jain University, Kanakpura Taluk, Ramanagara 562112, India

Abstract

Objective: Breast cancer is the second leading cause of death among cancer pateints. Ayurvedic system of traditional medicine validated the use of plants as a useful source for occurrence of anticancer compounds. This study was attempted to investigate the in vitro anti-cancer potential of methanolic extract of Aleo vera on breast cancer MCF-7 cell lines.

Methods: The methanol extract of Aleo vera was screened for its anti-proliferative effect against MCF-7 (Breast cancer) cell lines seeded on 96 well plates.

Results: MCF-7 cells were treated with the extract for 24h and 72h at a range of increasing concentrations (0-500μg/ml) in order to obtain a dose-response graph and IC50 value. The Aleo vera extract showed cytotoxic effect in MCF-7cells with IC50 of 74.33 μg/ml.

Conclusion: The Aleo vera extract showed effective cytotoxic activity in a dose and time dependent manner. Future investigations will be interesting to find an active principle and also better understand its mechanism of action against cancer.

 

Keywords: Aleo vera, Anticancer, Breast cancer, Cytotoxic, Antraquninones, Drugs

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References

1. Murthy NS, Agarwal UK, Chaudhry K, Saxena S. A study on time trends in incidence of breast cancer–Indian scenario. Eur J Cancer Care 2007;16:185-6.
2. Hortobagyi GN, De la Garza Salazar J, Pritchard K, Amadori D, Haidinger R, Hudis CA, Khaled H, et al. The global breast cancer burden: variations in epidemiology and survival. Clin Breast Cancer 2005;6:391-401.
3. Price P, Sikora K, Illidge T. Treatment of Cancer. 5 th ed. London: Hodder Arnold Publication; 2008.
4. Gossell-Williams M, Simon OR, West ME. The past and present use of plants for medicines. West Indian Med J 2006;55:217–8.
5. Shrestha S, Subaramaihha SR, Subbaiah SG, Eshwarappa RS, Lakkappa DB. Evaluating the antimicrobial activity of methanolic extract of rhus succedanea leaf gall. Bioimpacts 2013;3(4):195-8.
6. Stankovic MS, Curcic MG, Zizic JB, Topuzovic MD, Solujic SR, Markovic SD. Teucrium plant species as natural sources of novel anticancer compounds: antiproliferative, proapoptotic and antioxidant properties. Int J Mol Sci 2011;12:4190–205.
7. Reddy L, Odhav B, Bhoola KD. Natural products for cancer prevention: a global perspective. Pharmacol Ther 2003;99:1–13.
8. Guo X, Zhu K, Zhang H, Yao H. Anti-tumor activity of a novel protein obtained from tartary buckwheat. Int J Mol Sci 2010;11:5201–11.
9. Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol 2005;100:72–9.
10. Tan G, Gyllenhaal C, Soejarto DD. Biodiversity as a source of anticancer drugs. Curr Drug Targets 2006;7:265–77.
11. Gupta VK, Malhotra S. Pharmacological attribute of Aloe vera: Revalidation through experimental and clinical studies. Ayu 2012;33:193-6.
12. Harlev E, Nevo E, Lansky EP, Ofir R, Bishayee A. Anticancer potentail of aloes: antioxidant and antiproliferalive and immunostimulatory attributies. Planta Med 2012;78(9):843-52.
13. Lee YS, Ju HK, Kim YJ, Lim TG, Uddin MR, Kim YB, et al. Enhancement of anti-inflammtory activity of aloe vera adventitious root extracts through the alteration of primary and secondary metabolities via salicylic acid elicitation. PLoS One 2013;.8(12):e82479.
14. Amoo SO, Aremu AO, Van Staden J. Unraveling the medicinal potential of South African Aleo species. J Ethnopharmacol 2014;153(1):19-41.
15. El-Shemy HA, Aboul-Soud MA, Nassr-Allah AA, Aboul-Enein KM, Kabash A, et al. Antitumor properties and modulation of antioxidant enzymes activity by Aloe vera leaf active principles isolated via supercritical carbon dioxide extraction. Curr Med Chem 2010;17(2):129-38.
16. Mossman T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
17. Edmondson J, Armstrong L, Martinez A. A rapid and simple MTT-based spectrophotometric assay for determining drug sensitivity in monolayer cultures. J Tissue Cult Methods 1988;11:15-7.
18. Lin KY, Uen YH. Aloe-emodin, an anthraquinone, in vitro inhibits proliferation adn induces apoptosis in human colon carcinoma cells. Oncol Lett 2010;1(3):541-7.
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How to Cite
Srihari, R., A. R. Surendranath, N. Kasturacharya, K. C. Shivappa, N. D. Sivasitambaram, and B. L. Dhananjaya. “EVALUATING THE CYTOTOXIC POTENTIAL OF METHONOLIC LEAF EXTRACT OF ALEO VERA ON MCF-7 BREAST CANCER CELL LINES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, May 2015, pp. 81-83, https://innovareacademics.in/journals/index.php/ijpps/article/view/4020.