EVALUATING THE ANTIPROLIFERATIVE POTENTIAL OF METHONOLIC LEAF EXTRACT OF CASSIA NIGRICANS

  • Renuka Srihari Department of Biochemistry, Biotechnology, Maharani Lakshmi Ammanni College For Women, Bangalore, India
  • Austin Richard University of Mysore, Mysore 570006, Karnataka, India
  • Nandashree K Department of Biochemistry, Biotechnology, Maharani Lakshmi Ammanni College For Women, Bangalore, India
  • Karigar Cs Tumkur University, Tumkur, Karnataka, India
  • Niranjali Devaraj S University of Madras, Guindy campus, Chennai, Tamilnadu, India
  • Dhananjaya Bl Toxinology/Toxicology and Drug Discovery Unit, Centre for Emerging Technologies (CET), Jain University, Kanakpura Taluk, Ramanagara-562112, India.

Abstract

Objective: It is well established that plants have always been useful source as anticancer compounds. This study was attempted to investigate the in vitro anti-cancer potential of methonolic extract of Cassia nigricans on breast cancer MCF-7 cell lines.

Methods: The methanol extract of C. nigricans was screened for its anti-proliferative effect against MCF-7 (Breast cancer) cell lines using MCF-7 cells seeded 96 well plates.

Results: Extract was exposed with MCF-7 cell lines for 24h and 72h at a range of increasing concentrations (0-500μg/ml) in order to obtain a dose-response graph and IC50 value. The C. nigricans extract showed cytotoxic effect in MCF-7cells with IC50 of 82.6μg/ml.

Conclusion: The C. nigricans extract showed effective cytotoxic activity in a dose and time dependent manner. Future work will be interesting to know the chemical composition and also better understand the mechanism of action present in the extract for developing it as drug for therapeutic application.

 

Keywords: Cassia nigricans, Anticancer, Breast cancer, Cytotoxic, Antraquninones, Drugs

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How to Cite
Srihari, R., A. Richard, N. K, K. Cs, N. D. S, and D. Bl. “EVALUATING THE ANTIPROLIFERATIVE POTENTIAL OF METHONOLIC LEAF EXTRACT OF CASSIA NIGRICANS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, May 2015, pp. 154-5, https://innovareacademics.in/journals/index.php/ijpps/article/view/3707.