THE ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF THE LEAVES EXTRACT OF CLERODENDRUM COLEBROOKIANUM WALP, (FAM: VERBENACEAE)

  • Mahesh M Azyme Biosciences Private Limited, Jayanagar, Bangalore 560069, India
  • Preenon Bagchi Jain University
  • Lalthanzama Vanchhawng Jain University
  • Somashekar R Jain University
  • Ravi Shankara Be Jain University
  • Benaka Prasad Sb Jain University
  • Richard Sa Jain University
  • Dhananjaya Bl Jain University

Abstract

  1. Objective: To investigate the in-vitro antioxidant and antimicrobial potential of Clerodendrum colebrookianum leaves extract.

    Methods: The leaves of C. colebrookianum were collected from various parts of Aizawl, Mizoram, India. Subsequently, the leaves were extracted with solvents (chloroform, acetone, ethanol and methanol) in a Soxhlet extraction apparatus for 24hr. Further, the extracts were extensively examined for its in-vitro antioxidant (DPPH) and antimicrobial activities. The preliminary phytochemical screening was carried out using standard protocols.

    Results: The existence of alkaloids, flavonoids, diterpenes, saponins, glycosides, steroids and terpeinoids were revealed in the phytochemical screening. The aqueous and acetone extract had the highest total phenolic content (2.348 mg/ml), when compared to methanol, ethanol and chloroform extracts, which was 0.549 mg/ml, 0.408 mg/ml and 0.407 mg/ml, respectively. The antioxidant activity was more significant for aqueous extract, when compared to other extracts. The antimicrobial activity was more significant for acetone extract showed significant zone of inhibition of 14±0.3, 13±0.3 and 15±0.2 for E. coli, S. marcescens and S. aureus, respectively.

    Conclusion: The high level of antioxidant and antimicrobial potential of C. colebrookianum leaf extracts encourage its potential use for biomedical applications.

Keywords: Plants, Gallic acid, Drugs, Extracts

Downloads

Download data is not yet available.

References

1. Gossell-Williams M, Simon OR, West ME. The past and present use of plants for medicines. West Indian Med J 2006;55:217–8.
2. Hugo WB, Russel AD. Pharmaceutical Microbiology. 3th ed. Oxford: Blackwell Scientific Publications; 1984. p. 179-200.
3. Melnik S, Stoger E. Green factories for biopharmaceuticals. Curr Med Chem 2013;20:1038-46.
4. Carounanidy U, Satyanarayanan R, Velmurugan A. Use of an aqueous extract of Terminalia chebula as an anticaries agent: a clinical study. Indian J Dental Res 2007;18:152–6.
5. Cohen ML. Epidemiology of drug resistance: implications for a post antimicrobial era. Science 1992;257:1050-5.
6. Nascimento Gislene GF, Juliana L, Paulo CF. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz J Microbiol 2000;31:247-56.
7. Halliwell B. Antioxidants and human disease: a general introduction. Nut Rev 1997;55:44–52.
8. Pham Huy LA, He H, Pham-Huy C. Free radicals, Antioxidants in disease and health. Int J Biomed Sci 2008;4:89-96.
9. Kannan P, Ramadevi SR, Hopper W. Antibacterial activity of Terminalia chebula fruit extract. Afr J Microbiol Res 2009;3:180–4.
10. Erturk O. Antibacterial and antifungal effects of alcoholic extracts of 41 medicinal plants growing in Turkey. Czech J Food Sci 2010;28:53–60.
11. Ye JM, Stanley MH. Strategies for the discovery and development of anti-diabetic drugs from the natural products of traditional medicines. J Pharm Pharm Sci 2013;16:207-16.
12. Sharma HK, Chhangte L, Dolui AK. Traditional medicinal plants in Mizoram, India. Fitoterapia 2001;72:146-61.
13. Jamir TT, Sharma HK, Dolui AK. Folklore medicinal plants of Nagaland, India. Fitoterapia 1999;72:395-401.
14. Namsa ND, Mandal M, Tangjang S. Ethnobotany of the Monpa ethnic group at Arunachal Pradesh, India. J Ethnobiol Ethnomed 2011;7:31.
15. Singh NR, Singh MS. Wild medicinal plants of manipur included in the red list. Asian Agri-History 2009;13:221-5.
16. Adeneye AA, Adekele TI, Adeneye AK. Hypoglycemic and hypolipidemic effects of the aqueous fresh leaves extract of Clerodendrum capitatum in Wistar rats. J Ethnopharmacol 2008;116:7-10.
17. Devi R, Sharma DK. Hypolipidemic effect of different extracts of Clerodendron colebrookianum Walp in normal and high-fat diet fed rats. J Ethnopharmacol 2004;90:63-8.
18. Jadeja RN, Thounaojam MC, Ramani UV. Anti-obesity potential of Clerodendron glandulosum Coleb. leaf aqueous extract. J Ethnopharmacol 2011;135:338-43.
19. Richardson PM, Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 2nd ed. Brittonia; 1990. p. 115.
20. Trease G, Evans W. Pharmacognosy. 13th ed. Delhi: ELBS Publication; 1989.
21. Kokate A. Phytochemical methods. Phytotherapy. 2nd ed; 1999;78:126–8.
22. Khandelwal KR. Practical Pharmacognosy: Techniques and Experiments. 13th ed. Pune: Nirali Prakashan; 2005.
23. Kaur C, Kapoor HC. Anti‐oxidant activity and total phenolic content of some Asian vegetables. Int J Food Sci Technol 2002;37:153-61.
24. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199–200.
25. Srinivasan D, Sangeetha N, Suresh T. Antimicrobial activity of certain Indian medicinal plants used in folkloric medicine. J Ethnopharmacol 2001;74:217-20.
26. Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turk J Biol 2005;29:41-7.
27. Collins CH, Lyne PM, Grange JM. Microbiological Methods. 6th ed. London: Butterworhs Co Ltd; 1989. p. 410.
28. Naik GH, Priyadarsini KI, Satav JG. Comparative antioxidant activity of individual herbal components used in ayurvedic medicine. Phytochemistry 2003;63:97–104.
29. Sultana B, Anwar F, Przybylski R. Antioxidant activity of phenolic components present in barks of barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and eugenia jambolana lam trees. Food Chem 2007;104:1106–14.
30. Choi Y, Jeong HS, Lee J. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 2007;103:130–8.
31. Shrestha S, Subaramaihha SR, Subbaiah SG. Evaluating the antimicrobial activity of methanolic extract of rhus succedanea leaf gall. Bioimpacts 2013;3:195-8.
32. Decker A. Phenolics: prooxidants or antioxidants? Nutr Rev Wiley Online Library; 1997;55:396–8.
33. Rice-Evans CA, Miller NJ, Bolwell PG. The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic Res 1995;22:375–83.
34. Tschesche R. Advances in the chemistry of antibiotics substances from higher plants: Pharmacognosy and phytochemistry. In: Wagner H, Horharmmer L. editors. Proceeding of the 1st International Congress, Murich. Berlin; 1971. p. 274-89
Statistics
395 Views | 803 Downloads
How to Cite
M, M., P. Bagchi, L. Vanchhawng, S. R, R. S. Be, B. P. Sb, R. Sa, and D. Bl. “THE ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF THE LEAVES EXTRACT OF CLERODENDRUM COLEBROOKIANUM WALP, (FAM: VERBENACEAE)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 13, Aug. 2015, pp. 96-99, https://innovareacademics.in/journals/index.php/ijpps/article/view/6262.