THE ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF THE LEAVES EXTRACT OF CLERODENDRUM COLEBROOKIANUM WALP, (FAM: VERBENACEAE)
Keywords:
Plants, Gallic acid, Drugs, ExtractsAbstract
Objective: To investigate the in-vitro antioxidant and antimicrobial potential of Clerodendrum colebrookianum leaves extract.
Methods: The leaves of C. colebrookianum were collected from various parts of Aizawl, Mizoram, India. Subsequently, the leaves were extracted with solvents (chloroform, acetone, ethanol and methanol) in a Soxhlet extraction apparatus for 24hr. Further, the extracts were extensively examined for its in-vitro antioxidant (DPPH) and antimicrobial activities. The preliminary phytochemical screening was carried out using standard protocols.
Results: The existence of alkaloids, flavonoids, diterpenes, saponins, glycosides, steroids and terpeinoids were revealed in the phytochemical screening. The aqueous and acetone extract had the highest total phenolic content (2.348 mg/ml), when compared to methanol, ethanol and chloroform extracts, which was 0.549 mg/ml, 0.408 mg/ml and 0.407 mg/ml, respectively. The antioxidant activity was more significant for aqueous extract, when compared to other extracts. The antimicrobial activity was more significant for acetone extract showed significant zone of inhibition of 14±0.3, 13±0.3 and 15±0.2 for E. coli, S. marcescens and S. aureus, respectively.
Conclusion: The high level of antioxidant and antimicrobial potential of C. colebrookianum leaf extracts encourage its potential use for biomedical applications.
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