INHIBITION OF MULTI-TOXIC PHOSPHOLIPASE A2 OF DABOII RUSELLII PULCHELLA VENOM BY THE ETHANOLIC EXTRACT OF A. PANICULATA
Objective: Andrographis paniculata (A. paniculata) Nees is an important medicinal plant found in the tropical regions and has been traditionally used in Indian and Chinese medicinal systems. A. paniculata is found to exhibit anti-snake venom properties; however its inhibitory potential on multi-toxic Phospholipases A2 and its associated inflammatory reactions is not clearly understood. Henceforth, in this study we evaluate the inhibitory/neutralizing potential of alcoholic extract of A. paniculata on the isolated multi-toxic PLA2 (of Daboii rusellii pulchella and Naja naja) and its induced edema in Swiss albino mice.
Methods: The multi-toxic-svPLA2-VRV-PL-V from the venom of Daboii rusellii pulchella was purified according established methods. The inhibition of enzymatic activity and edema inducing activity was carried out according to established methods.
Results: A. paniculata extract dose dependently inhibited the multi-toxic svPLA2 enzymaticactivity with an IC50 value of 12.4Â±0.6 Âµg/ml. Further, the extract dose dependently inhibited the edema formation, when co-injected with the enzyme indicating a strong correlation between lipolytic activity and pro-inflammatory activity.
Conclusion: The ethanolic extract of A. paniculata effectively inhibited the multi-toxic svPLA2 and its associated edema inducing activities, which substantiate their anti-ophidian properties. Further study is interesting to develop them into potent anti-ophidian agents.
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