IN-VITRO CYTOTOXIC EFFECT OF CANTHIUM DICOCCUM ON DIFFERENT CANCER CELL LINES
Objective: Cancer remains one of the most dreaded diseases causing an astonishingly high death rate. Despite the use of surgical resection and aggressive chemotherapy, nearly 50% of patients with carcinoma develop recurrent disease, highlighting the need for improved therapies. Henceforth, complementary and alternative medicine is slowly emerging as an option. A variety of ingredients of traditional medicines and herbs are being widely investigated in several parts of the world to analyze their potential as therapeutic agents against cancer. In the present study we investigated the efficacy of methanol extracts of Canthium dicoccum, for its clonogenic inhibition on Human Breast cancer (MD-MB-231), Prostate cancer (PC-3) and Lung cancer (Calu-6) cell lines.
Methods: The cytotoxic effect of methanolic extract of Canthium dicoccum was evaluated by MTT assay on MD-MB-231, Calu-6, and PC-3 cells.
Results: The methanol extract of C. dicoccum showed significant cytotoxicity against MD-MB-231and Calu-6, when compared to PC-3 cells.
Conclusion: The methanol extracts of C. dicoccum showed effective cytotoxic activities in a dose and time dependent manner. Future work will be interesting to know the chemical composition and also better understanding the mechanism of action will help in developing it as drug for therapeutic application.
2. Patwardhan B, Vaidya ADB. Natural product drug discovery: Accelerating the clinical candidate development using reverse pharmacology approaches. Indian J Exp Biol 2010;48:220â€“7.
3. Dev S. Impact of natural products in modern drug development. Indian J Exp Biol 2010;48:191â€“8.
4. Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol 2005;100:72â€“9.
5. Tan G, Gyllenhaal C, Soejarto DD. Biodiversity as a source of anticancer drugs. Curr Drug Targets 2006;7:265â€“77.
6. Raja Rajeshwari, Ramalakshmi S, Muthuchelian K. GC-MS Analysis of bioactive components from the ethanolic leaf extract of Canthium dicoccum (Gaertn.) Teijsm and Binn. J Chem Pharm Res 2011;3:792-8.
7. Neelima M, Prasad GP, Sudarsanam G, Penchala PG, Jothi B. Ethnobotanical studies in rapur forest division of nellore district in Andhra Pradesh. Life Sci Leaflets 2011;11:335-45.
8. Vuyyuri B, Tripurana R, Gangeyula J, Ali F. Anti-inflammatory activity of ethanolic extract of Canthium Dicoccum. Int J Pharm Phytopharm Res 2013;3:226-30.
9. Santhan S, Janartan S, Zuber Ali M. Evaluation of Anti-diabetic and nephro protective activity of 95% ethanolic extract of Canthium dicoccum whole plant using albino rats. J Chem Pharm Sci 2013;6:218-22.
10. Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. 3rd edition. Chapman and Hall pub. London. UK; 1998.
11. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
12. Edmondson JM, Armstrong LS, Martinez AO. A rapid and simple MTT-based spectrophotometric assay for determining drug sensitivity in monolayer cultures. J Tissue Cult Methods 1988;11:15-7.
13. Pandey KB, Rizvi SI. Plant polypenols as dietary antioxidants in human health and disease. Oxid Med Cell Longevity 2009;2:270-8.
14. LeÃ³n-GonzÃ¡lez AJ, Auger C, Schini-Kerth VB. Pro-oxidant activity of polyphenols and its implications on cancer chemoprevention and chemotherapy. Biochem Pharmacol 2015. doi:10.1016/j.bcp.2015.07.017. [Article in Press]