SCREENING FOR BIOACTIVES FROM INDIAN MEDICINAL HERBS – A SIMPLISTIC APPROACH FOR ANTIOXIDANT METABOLITES
Keywords:
Herbal extracts, Cytoprotective, Antioxidant, Anti-rhizopus, DPPH, OxidationAbstract
Herbal extracts obtained from 20 Indian medicinal plants were evaluated for their cytoprotectivity on erythrocytes and antioxidant properties. Total phenol content and anti-rhizopus activity were also determined. Results indicated that, out of 20 extracts evaluated, radical scavenging capacity and anti-rhizopus activity were observed in aqueous extract of Ocimum tenuiflorum, Leucas aspera, Terminalia arjuna, Glycyrrhiza glabra and Nyctanthes arbortristis in a dose dependent manner. The total phenolic content was observed to be 1289, 3837, 372, 2831 and 1892 μg GAE/g for O. tenuiflorum, L. aspera, T. arjuna, G. glabra and N. arbortristis respectively. The antioxidant activity correlates with the phenolic content of the extracts. At 1 mg/ml the above extracts showed 98% protection on erythrocyte cell oxidation. These results demonstrate that the cytoprotectivity and antioxidant potency of these extracts could be the basis for their alleged health promoting potential. These herbs could serve as new sources of natural antioxidants or nutraceuticals with potential applications in reducing oxidative stress conditions.
Â
Downloads
References
Meghashri S, Vijay Kumar H, Gopal S. Antioxidant properties of a novel flavonoid from leaves of Leucas aspera. Food Chem 2010; 122, 105-110.
Esra M, Salih Y. Evaluation of Phytochemicals and Antioxidant Activity of Ginkgo biloba from Turkey. Pharmacologia 2012; 3, 113-120.
Roselli M, Lentini G, Habtemariam S. Phytochemical, Antioxidant and Anti-α-glucosidase activity Evaluations of Bergenia cordifolia. Phytotherapy Res doi: 2011; 10.1002/ptr.3655.
Imaida KF, Shivai S, Ohtani T, Nakanish M, Ito K. Promoting activities of butylated hydroxyanisole and butylated hydroxytoulene on 2-stage urinary bladder carcinogenesis and inhibition of γ-glutamyl transpeptidase-positive foci development in the liver of rats. Carcinogenesis1983; 4, 895-899.
Wong PYY, Kitts DD. Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinum crispum) and cilantro (Coriandum sativum) extracts. Food Chem 2006; 97, 505-515.
Northover J, Zhou T. Control of rhizopus tor of peaches with postharvest treatments of tebuconazole, fludioxonil and Pseudomonas syringae. Can J Plant Path 2002; 24, 144-153.
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958; 18, 1199-1200.
Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. Springer 1996; Berlin.
Suwalsky M, Orellana P, Avello M, Villena F. Protective effect of Ugni molinae Turcz against oxidative damage of human erythrocytes. Food Chem Toxicol 2007; 45, 130-135.
Zhang ZZ, Li YB, Qi L, Wan XC. Antifungal activities of major tea leaf volatile constituents toward Colletorichum camelliae Massea. J Agri Food Chem 2006; 54, 3936-3940.
Lima CF, Valentao PCR, Andrade PB, Seabra RM, Ferreira MF, Wison CP. Water and methanolic extracts of Salvia officinalis protect HepG2 cells from t-BHP induced oxidative damage. Chem-Biol Inter 2007; 167, 107-115.
Battistelli M, Sanctis R, De Bellis R, Cucchiarini L, Dacha M, Gobbi P. Rhodiola rosea as antioxidant in red blood cells: Unstructural and hemolytical behavior. European J Histochemistry 2005; 49, 243-245.
Jyoti Sethi, Sushma Sood, Shashi Seth, Anjana Talwar. Evaluation of hypoglycemic and antioxidant effect of Ocimum Sanctum. Indian J Clinical Biochem 2004; 19, 152-155.