EFFICIENCY OF CURCUMIN AND CHITOSAN NANOPARTICLES AGAINST TOXICITY OF POTASSIUM DICHROMATE IN MALE MICE
Background: Potassium dichromate is a dangerous ecological pollutant implicated in the reproductive toxicity and nephrotoxicity. Nanoparticles currently have been widely used as nanotherapeutics.
Objective: The purpose of this work was to examine the protective effect of nanocurcumin and nanochitosan supplementation on potassium dichromate induced toxicity in male mice.
Methods: Mice were divided into six groups, first group received saline. Second and third groups were given orally nanocurcumin and nanochitosan respectively for 5 days. Fourth group was injected subcutaneously with a single dose of potassium dichromate for 24 h. Group five and six were administrated nanocurcumin and nanochitosan respectively prior to potassium dichromate. Bone marrow was harvested for chromosomal examination and epididymal sperms was collected for sperm morphology while Kidneys and testes were collected for western blot and biochemical analysis.
Results: Potassium dichromate induced significant increase in chromosome and sperm abnormalities as well as testicular and renal MDA, renal MPO, renal contents of IL-18 and IGF-1, testicular contents of caspase 3 and cytosolic cytochrome c, a reduction in testosterone level, and GPx of renal and testicular tissues. Pretreatment with nanoparticles showed significant mitigation against alterations induced by potassium dichromate particularly nanochitosan was more effective.
Conclusion: The present study suggested that the selected nanoparticles have antioxidant and antigenotoxic properties against toxicity of potassium dichromate.
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