ANALYSIS OF BIO-ACTIVE COMPOUNDS PRESENT IN THE LEAVES AND STEM OF TRICHOSANTHES LOBATA ROXB. USING GC-MS TECHNIQUE WITH RESPECT TO ITS ANTI-INFLAMMATORY ACTION
BIO-ACTIVE COMPOUNDS OF TRICHOSANTHES LOBATA ROXB
The utilization of various parts of Trichosanthes lobata such as leaf and stem in different folklore medicaments is well renown. Phytochemistry and pharmacology plays a key role in providing scientific validation to such reports and channelizes the medicinal chemist in optimization of drug discovery to invent new drugs. Scientific validations can be given through in vitro and in vivo pharmacological studies, whereas lead identifications can be done through exploring its phytochemistry. In this regard the structural elucidation studies on Trichosanthes lobata ethyl acetate and methanol extracts of leaf and stem parts were done using Gas Chromatography – Mass Spectrometry (GC-MS) technique. Extracts were obtained with shade dried and finely powdered samples in successive solvent extraction using ethyl acetate and methanol solvents by Soxhlet apparatus. The study revealed that the extraction solvent used was able to recover compound of classes such as organic acid esters and conjugated alkaloids in larger quantities than other class of compounds. Also, the set of compounds present in the extract varied with leaf and stem and also with polarity of solvents used. In total compounds identified, GC-MS profile of Ethyl acetate leaf extract of T. lobata contained 41, stem extract contained 45 compounds which have reported bioassays in PubChem. Whereas GC-MS profile of methanol leaf extract of T. lobata contained 66 and stem extract contained 46 compounds having bioassay reports in PubChem. A large number of phytochemical peaks with good area percentage was found in methanolic extract. We were also able to find out potent anti-inflammatory compounds such as Octanoic acid, Dodecanoic acid, Octadecane, Enoic acid, Hexanoic acid, Quinazolin-8-one, Ilicic acid, Pentadecanoic acid, Oxaspiro, Benzeneacetic acid etc. from the extracts. The findings of this study suggest that T. lobata contains phytocompounds which may serve as new drug leads of natural products origin in future and make it employable in modern pharmacological practices.
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