SCREENING OF ANTIBIOFILM AND ANTI-QUORUM SENSING POTENTIAL OF VITEX TRIFOLIA IN PSEUDOMONAS AERUGINOSA

  • R. Nancy Immaculate Mary Research Scholar Department of Biotechnology, Vels University, Chennai, Tamilnadu, India
  • N. Banu Associate Professor, Department of Biotechnology, Vels University, Chennai, Tamilnadu, India

Abstract

Objective: In this present study the methanolic leaf extract of Vitex trifolia was assessed for its anti quorum sensing activity against acyl-homoserine lactone (AHL)-dependent virulence factors in Pseudomonas aeruginosa.

Methods: In this study the methanolic leaf extract of Vitex trifolia was screened to control the virulence factors of Pseudomonas aeruginosa by antibacterial analysis, biofilm prevention assay, protease and elastase assay, pyocyanin production, EPS (Exopolysaccaride) assay, Swimming assay and Light microscopic analysis.

Results: The methanolic leaf extract obtained from Vitex trifolia exhibited a concentration dependent (50-100 μg/ml) reduction in anti quorum sensing activity in Pseudomonas aeruginosa. The extract inhibited 69% of protease activity, 48% of elastase activity and 96% of exopolysaccharide production in Pseudomonas aeruginosa. The pigment pyocyanin production and swimming motility of the bacteria were also controlled.

Conclusion: Thus in this study the methanolic leaf extract of Vitex trifolia was found to be potent against anti quorum sensing activity in Pseudomonas aeruginosa.

 

Keywords: Vitex trifolia, Quorum sensing inhibition, Acyl homoserine lactone, Pseudomonas aeruginosa, Antibacterial assay, Antibiofilm

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How to Cite
Mary, R. N. I., and N. Banu. “SCREENING OF ANTIBIOFILM AND ANTI-QUORUM SENSING POTENTIAL OF VITEX TRIFOLIA IN PSEUDOMONAS AERUGINOSA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, June 2015, pp. 242-5, https://innovareacademics.in/journals/index.php/ijpps/article/view/4113.
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