A STUDY OF CHLOROPHYLLIN OF MEDICINAL PLANTS, ITS CHEMICAL CHARACTERIZATION AND ANTI–PROLIFERATIVE ACTIVITY WITH SPECIAL REFERENCE TO SOLANUM TRILOBATUM L. ON LIVER CELL LINE


N. Banu, Pavithra S.

Abstract


Objective: Plants are the richest source of bioactive compounds and they have been used as medicine also. Chlorophyllin (CHL) is water-soluble derivative of chlorophyll (chl) in which magnesium has been replaced with copper and the phytol chains lost. Chlorophyllin has been used by human population for over 50 y for medicinal purposes with no adverse effects. Chlorophyllin is a promising chemo preventive agent to block cancer primarily by inhibiting carcinogen such as AFB1. The objective was to extract the bioactive pigment chlorophyllin from medicinal plants and to study its anticarcinogenic property on liver cell lines.

Methods: In the present study the bioactive pigment, chlorophyllin was extracted and estimated from six medicinal plant leaves and characterized by IR and NMR. Further, based on the high chlorophyllin content (12.21µg/ml), Solanum trilobatum L. was selected for the study of anticarcinogenic property against two types of cell lines: HepG2 cell lines (Human Hepatocellular Carcinoma) and Vero cell lines (African Green Monkey kidney).

Results: It was found that the inhibitory effect of chlorophyllin was found on cancer cell lines (IC50 value at 48H was 62.5μg/ml) and absent on Vero cell lines. Standard chlorophyllin was used as control for all the studies.

Conclusion: This is the first report on the effect of natural chlorophyllin from the leaves of Solanum trilobatum L. on HepG2 cell lines. The in vitro data suggests that the consumption of the leaves of Solanum trilobatum L. or as chlorophyllin may impart anticancer effects.

 


Keywords


Chlorophyllin, Solanum trilobatum L., Hepato Cellular Carcinoma, Vero cell lines

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References


McGlynn A, Tsao L, Hsing AW, Devesa S, Fraumeni F. International trends and patterns of primary liver cancer. Int J Cancer 2001;94:290-6.

Kaufmann SH, Earnshaw WC. Induction of apoptosis by cancer chemotherapy. Exp Cell Res 2000;256:42-9.

Dashwood RH, Pereira C, Bailey GS, Williams D, Jubert C, Mata J, et al. Effects of chlorophyll and chlorophyllin on low dose aflatoxin B1 pharmacokinetics in human volunteers: a pilot study. Cancer Prev Res 2009;14:1-21.

Waters MD, Jackson AM, Brockman EH. Activity profiles of antimutagens: in vitro and in vivo data. Mutat Res 1996;350:109-29.

Pezzuto JM. Plant-derived anti-cancer agents. Biochem Pharmacol 1997;53:121-33.

Schertz FM. The extraction and separation of Chlorophyll (α+β) carotene and xanthophyll in fresh green leaves, preliminary to their quantitative determination. Plant Physiol 1928;3:211–6.

Mossman T. Rapid colorimetric assay for cellular growth and survivals: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;3:21–9.

Breinholt V, Hendricks J, Pereira C. Dietary chlorophyllin is a potent inhibitor of aflatoxin B1 hepatocarcinogenesis in rainbow trout. Cancer Res 1995;55:57-62.

Feruzzi GM, Blakeslee J. Digestion, absorption and cancer preventive activity of dietary chlorophyll derivatives. Nutr Res 2007;27:1–12.

Chernomorsky S. Chlorophyllin copper complex: quality control. J Soc Cosmet Chem 1993;44:235–8.

Kumar SS, Shankar B, Sainis KB. Effect of chlorophyllin against oxidative stress in spleen lymphocytes in vitro and in vivo. Biochim Biophys Acta 2004;1672:100-11.

Jacobs EE, Holt AS. Infra-red absorption spectra of chlorophylls and derivatives. Plant Physiol 1955;30:553-9.

Girish C. Antiulcer activity of aqueous extract of leaves of Scoparia dulcis (Linn.) in rats. J Pharm Res 2011;4:2526-8.

Noriko O, Nam HD, Emi K, Akira K, Satoshi I, Chikao M. Aqueous extract of Carica papaya leaves exhibits anti-tumor activity and immunomodulatory effects. J Ethnopharmacol 2009;127:760-7.

Chang CH, Wu HC, Wang RF. Significant association of methylenetetrahydrofolate reductase single nucleotide polymorphism with prostate cancer susceptibility in Taiwan. Anticancer Res 2010;30:3573-7.




About this article

Title

A STUDY OF CHLOROPHYLLIN OF MEDICINAL PLANTS, ITS CHEMICAL CHARACTERIZATION AND ANTI–PROLIFERATIVE ACTIVITY WITH SPECIAL REFERENCE TO SOLANUM TRILOBATUM L. ON LIVER CELL LINE

Keywords

Chlorophyllin, Solanum trilobatum L., Hepato Cellular Carcinoma, Vero cell lines

Date

27-10-2015

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 7, Issue 12, 2015 Page: 69-79

Online ISSN

0975-1491

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156 Views | 418 Downloads

Authors & Affiliations

N. Banu
Vels University, Velan Nagar, P. V. Vaithiyalingam Road, Pallavaram, Chennai 117, Tamil Nadu, India
India

Pavithra S.
Department of Biotechnology, Vels University, Velan Nagar, P. V. Vaithiyalingam Road, Pallavaram, Chennai 117, Tamil Nadu, India
India


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