Phenotypic and Genotypic characterization of Chlorella species isolated from Environmental samples.
Application and Standardization of Uniplex PCR targeting Chlorella species
Microalgae with beneficial compounds available in the environment are rich source for cost effective pharmaceutical and food industries. Isolation and identification of Chlorella species from the environment through phenotypic method requires technical expert with great knowledge on the Algal biology. In this 21st century, advancement of Molecular identification plays vital role in identification of any Microorganisms from genus to species level with higher accuracy and confidence rate than the phenotypic identification, which may give us false report based on the expertise of individual taxonomist. In this context, this study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region. We have standardized the in-house designed primer and optimized the PCR conditions to characterize Chlorella isolated from the environmental water samples. Further, we carried out Sanger sequencing for the identification of algae upto the genus and species level. Out of 2 algae samples identified phenotypically, one isolated showed to be Chlorella vulgaris and other one showed as chlorella sorokiniana. We have submitted the Chlorella vulgaris to NCBI and published the same. To conclude, this study provided a primers with PCR conditions to characterize algal samples through molecular identification with 100% accuracy than the phenotypic method.
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