IN VITRO STUDIES ON Î‘-GLUCOSIDASE INHIBITION, ANTIOXIDANT AND FREE RADICAL SCAVENGING PROPERTIES OF TECOMA STANS L.
Keywords:Nil, T stans, MeTs, Antioxidant etc
Objective: Tecomo. stans (T. stans) Â (L) are plants extensively used for the empirical treatment of diabetes mellitus. But the bark part of T. stans (L) has not been extensively studied for the anti-diabetic and antioxidant property. Hence the current study were designed to estimate the in vitro activity of Î±-glucosidase inhibition and antioxidant property from bark extracts of T stans using various solvents such as hexane, ethyl acetate and methanol.
Methods: In the current study crude extract of T stans L. bark part has been evaluated for In vitro Î±-glucosidase inhibition and antioxidant activity from hexane, ethyl acetate and methanol extract using standard methods.
Results: Out of three solvents studied, methanol extract of T. stans L. (MeTs) showed 50% Î±-glucosidase inhibition at the concentration of 645.20Â±2.79 Î¼g/ml. The total phenolic content of MeTs was 206.81Â±1.11 mg of catechol equivalents/g extract. MeTs showed great scavenging activity on 2, 2-diphenyl-picrylhydrazyl (DPPH) (IC50 765.25Â±0.42 Âµg/ml), hydroxyl (IC50 245.12Â±1.41 Î¼g/ml), nitric oxide (IC50 800.10Â±1.05Âµg/ml) and superoxide (IC50 595.15Â±0.59 Âµg/ml) radicals, as well as high reducing power. MeTs also showed a strong suppressive effect on lipid peroxidation (IC50 810.15Â±2.19 Âµg/ml).
Conclusion: The results obtained in this study clearly indicate that MeTs has a significant potential to use as a natural a-glucosidase inhibition, antioxidant agent.
Edeoga HO, Gomina A. Nutritional values of some non-conventional leafy vegetable in Nigeria. J Economic Taxonomic Botany 2000;24:7-13.
Kumar RA, Sridevi K, Kumar NV, Nanduri S, Rajagopal S. Anticancer and immuno stimulatory compounds from Andrographispaniculata. J Ethnopharmacol 2004;92:291-5.
Kaveeshwar, Seema Abhijeet, Jon Cornwall. The current state of diabetes mellitus in India. Australasian Med J 2015;45â€“8.
Mukherjee PK, Kumar V, Houghton PJ. Screening of Indian medicinal plants for acetyl cholinesterase inhibitory activity. Phytother Res 2007;21:1142-5.
Nagavani V, Rao TR. Evaluation of antioxidant potential and qualitative analysis of major polyphenols by RP-HPLC in NymphaeanouchaliBurm flowers. Int J Pharm Pharm Sci 2010;2:98-104.
Cartea ME, Francisco M, Lema M, Soengas P, Velasco P. Resistance of cabbage (Brassica oleraceacapitata group) crops to Mamestrabrassicae. J Econ Entomol 2010;103:1866-74.
Mundhe KS, Kale AA, Gaikwad SA, Deshpande NR, Kashalkar RV. Evaluation of phenol, flavonoid contents and antioxidant activity of Polyalthialongifolia. J Chem Pharm Res 2011;3:764-9.
Hakiman M, Maziah M. Non enzymatic and enzymatic antioxidant activities in aqueous extract of different Ficusdeltoidea accessions. J Med Plants Res 2009;3:120-31.
Marzouka M, Gamal-Eldeenb A, Mohamedc M, El-Sayedc M. Anti-Proliferative and Antioxidant Constituents from T. stans. ZeitschriftfÃ¼r Naturforschung C; 2006. p. 783-91.
Alanso-Castro JA, Zapata-Bustos R, Romo-Yanez J, Camarillo-Ledesma P, Gomez-Sanchez M, et al. The antidiabetic plant T. stans (L.) Juss. exKunth (Bignoniaceae) and TeucriumcubensJacq (Lamiaceae) induce the incorporation of glucose in insulin sensitive and insulin-resistant murine and human adipocytes. J Ethnopharmacol 2010;127:62-9.
Nimal Christhudas IVS, Praveen Kumar P, Sunil C, Vajravijayan S, Lakshmi Sundaram R, Jenifer Siril S, et al. In vitro studies on Î±-glucosidase inhibition, antioxidant and free radical scavenging activities of Hedyotisbiflora L. Food Chem 2013;138:1689-95.
Sunil C, Ignacimuthu S. In vitro and in vivo antioxidant activity of Symplocoscochinchinensis S. Moore leaves containing phenolic compounds. Food Chem Toxicol 2011;49:1604â€“9.
Dahlqvist A. Method for assay of intestinal disaccharides. Anal Biochem 1964;7:18â€“25.
Slinkard K, Singleton VL. Total phenol analyses: Automation and comparison with manual methods. Am J Enol Vitic 1977;8:4955.
Hanato T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: Their relative astringency and radical scavenging effects. Chem Pharm Bull 1988;36:2090â€“7.
Elizabeth K, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharm 1990;58:237â€“40.
Garratt DC. The quantitative analysis of drugs. Vol. 3. Japan: Chapman and Hall Ltd; 1964. p. 456â€“8.
Liu F, Ooi VEC, Chang ST. Free radical scavenging activities of mushroom polysaccharide extracts. Life Sci 1997;60:763â€“71.
Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japan J Nutr 1986;44:307â€“15.
Yen GC, Hsieh CL. Antioxidant activity of extracts from Du-zhong (Eucommiaurmoides) towards various peroxidation models in vitro. J Agric Food Chem 1998;46:3952â€“7.
Brand-Williams W, Cuvelier M, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft und Technol 1995;28:25â€“30.
Hochestein P, Atallah AS. The nature of oxidant and antioxidant systems in the inhibition of mutation and cancer. Mutat Res 1988;202:363â€“75.
Babu BH, Shylesh BS, Padikkala J. Antioxidant and hepato protective effect of Alanthusicici focus. Fitoterapia 2001;72:272â€“7.
Marcocci L, Packer L, Droy-Lefai MT, Sekaki A, Gardes-Albert M. Antioxidant action of Ginkgo biloba extracts EGb 761. Methods Enzymol 1994;234:462â€“75.
Aruoma OI. Free radicals, oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc 1998;75:199â€“212.
Janero DR. Malondialdehyde and thiobarbituric acid reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radicals Biol Med 1990;9:515â€“40.
Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in oxidative defence systems of various senescing leaves. J Agric Food Chem 1995;43:1813â€“7.
Nitin KU, Yogendra Kumar MS, Asheesh G. Antioxidant, cytoprotective and antibacterial effects of Sea buck-thorn (Hippophaerhamnoides L.) leaves. Food Chem Toxicol 2010;48:3443â€“8.