ANTIHYPERGLYCEMIC AND ANTIDYSLIPIDEMIC ACTIVITY IN ETHYL ACETATE FRACTION OF THE FRUITS OF XYLOCARPUS GRANATUM AND XYLOCARPUS MOLUCCENSIS
Objectives: Although various species of Xylocarpus i. e. Granatum, moluccensis are known for their medicinal properties. Yet, its anti-diabetic activity remains to be defined. So the aim of this study was to evaluate the antihyperglycemic and antidyslipidemic activity in ethyl acetate fraction of the fruits of X. granatum and X. moluccensis on validated animal models as well as in-vitro glucose uptake stimulatory effect and their cytotoxicity effect in L6 skeletal muscle cells.
Methods: The ethyl acetate fraction of the fruits of X. granatum and X. moluccensis were administered to diabetic groups daily up to 10 days for prolonged study. Biochemical parameters notably glucose tolerance, insulin level, lipid profile were assessed. The ethyl acetate fraction of the fruits of X. granatum and X. moluccensis were also tested for glucose uptake effect by skeletal muscle cells in the concentration dependent manner.
Results: The present study show that the ethyl acetate fraction of the fruits of X. granatum as well as X. moluccensis are effective in improving glucose tolerance, declining blood glucose as well as serum cholesterol and triglycerides levels in low dosed streptozotocin-induced diabetic rats and dyslipidemic hamsters, respectively. These fractions were also found efficient in increasing glucose uptake by L6 skeletal muscle cells but did not show any effect on cell viability of L6 skeletal muscle cells.
Conclusion: Based on the results, the present study revealed that ethyl acetate fraction of the fruits of X. granatum and X. moluccensis lowered blood glucose profile by increasing the glucose uptake by L-6 and this may be the possible mechanisms for the antidiabetic and antidyslipidemic action.
2. King H, Aubert RE, Herman WH. Global burden of diabetes, 1995â€“2025: Prevalence, numerical estimates, and projections. Diabetes Care 1998;21 Suppl 9:1414â€“31.
3. Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol 2002;8:81â€“100.
4. Bailey CJ, Day C. Traditional plant medicines as treatment for diabetes. Diabetes Care 1989;12:553â€“64.
5. Lakshmi V, Gupta P. An overview of the genus Xylocarpus. Nat Prod Res 2008;22 Suppl 14:1197-224.
6. Zaridah MZ, Idid SZ, Wan-Omar A, Khozirah S. In vitro antifilarial effects of three plant species against adult worms of subperiodic Brugia malayi. J Ethnopharmacol 2001;78:79â€“84.
7. Uddin SJ, Shilpi JA, Delazar A, Nahar L, Sarker SD. Free radical scavenging activity of some Bangladeshi plant extracts. Oriental Pharm Exp Med 2004;4 Suppl 3:187â€“95.
8. Wu J, Zhang S, Xiao Q, Li Q, Huang J, Xiao Z, et al. Xyloccensin M and N, two new B,D-seco limonoids from Xylocarpus granatum. Z Naturforsch B J Chem Sci 2003;58 Suppl 12:1216â€“9.
9. Wu J, Xiao Z, Song Y, Zhang S, Xiao Q, Ma C, et al. Spectral assignments and reference data: complete assignments of 1H and 13C NMR data for two 3, 8-epoxymexicanolides from the fruit of a Chinese mangrove Xylocarpus granatum. Magn Reson Chem 2006;44 Suppl 1:87â€“9.
10. Cui J, Deng Z, Li J, Fu H, Proksch P, Lin W. Phragmalin-type limonoids from the mangrove plant Xylocarpus granatum. Phytochem 2005;66 Suppl 19:2334â€“9.
11. Xiao Z, Wu J, Zhang S, Li Q. Xyloccensin K extracted from Xylocarpus granatum (muguodong) studied by NMR spectroscopy. Bopuxue Zazhi 2005;22 Suppl 3:315â€“9.
12. Uddin JS, Nahar L, Shilpi AJ, Shoeb M, Borkowski T, Gibbons S, et al. Gedunin, a Limonoid from Xylocarpus granatum, Inhibits the Growth of CaCo-2 Colon Cancer Cell Line In vitro. Phytother Res 2007;21:757â€“61.
13. Klip A, Guma A, Ramlal T, Bilan PJ, Lam V, Leiter LA. Stimulation of hexose transport by metformin in L6 muscle cells in culture. Endocrinol 1992;130:2535-44.
14. Tamarkar AK, Kumar R, Sharma R, Balapure AK, Lakshmi V, Srivastava AK. Stimulatory effect of Ceriops tagal on hexose uptake in L6 muscle cells in culture. Nat Prod Res 2008;22 Suppl 7:592-9.
15. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay. J Immunol Methods 1983;65 Suppl 1:55-63.
16. Yach D, Hawkes C, Gould CL, Hofman KL. The global burden of chronic diseases: overcoming impediments to prevention and control. J Am Med Assoc 2004;291:2616â€“22.
17. Rahuja N, Mishra A, Gautam S, Tamrakar AK, Maurya R, Jain SK, et al. Antidiabetic activity in flowers of nymphaea rubra. Int J Pharm Sci Rev Res 2013;22 Suppl 1;121-33.
18. Briones ER, Mao SJT, Palumbo PJ, Oâ€™Fallon WM, Chenoweth W, Kottke BA. Analysis of plasma lipids and lipoproteins in insulin dependent and non-insulin dependent diabetics. Metab 1984;33:42â€“9.
19. Koivisto UM, Martinez-Valdez H, Bilan PJ, Burdett E, Ramlal T, Klip A. Differential regulation of the GLUT1 and GLUT4 glucose transport systems by glucose and insulin in L6 muscle cells in culture. J Biol Chem 1991;266:2615-21.