ANTIHYPERGLYCEMIC AND ANTIDYSLIPIDEMIC ACTIVITY IN ETHYL ACETATE FRACTION OF THE FRUITS OF XYLOCARPUS GRANATUM AND XYLOCARPUS MOLUCCENSIS

  • Swayam Prakash Srivastava Division of Biochemistry
  • Neha Rahuja Division of Biochemistry
  • Savita Srivastava Division of Biochemistry
  • Akhilesh Kumar Tamrakar Division of Biochemistry
  • Sunil Kumar Mishra Division of Medicinal and Process Chemistry
  • Shishir Srivastava Division of Medicinal and Process Chemistry
  • Vijai Lakshmi Division of Medicinal and Process Chemistry
  • Mahendra Nath Srivastava Division of Botany, CSIR-Central Drug Research Institute
  • Arvind Kumar Srivastava Division of Biochemistry, CSIR-Central Drug Research Institute, Lucknow 226031, India.

Abstract

Objectives: Although various species of Xylocarpus i. e. Granatum, moluccensis are known for their medicinal properties. Yet, its anti-diabetic activity remains to be defined. So the aim of this study was to evaluate the antihyperglycemic and antidyslipidemic activity in ethyl acetate fraction of the fruits of X. granatum and X. moluccensis on validated animal models as well as in-vitro glucose uptake stimulatory effect and their cytotoxicity effect in L6 skeletal muscle cells.

Methods: The ethyl acetate fraction of the fruits of X. granatum and X. moluccensis were administered to diabetic groups daily up to 10 days for prolonged study. Biochemical parameters notably glucose tolerance, insulin level, lipid profile were assessed. The ethyl acetate fraction of the fruits of X. granatum and X. moluccensis were also tested for glucose uptake effect by skeletal muscle cells in the concentration dependent manner.

Results: The present study show that the ethyl acetate fraction of the fruits of X. granatum as well as X. moluccensis are effective in improving glucose tolerance, declining blood glucose as well as serum cholesterol and triglycerides levels in low dosed streptozotocin-induced diabetic rats and dyslipidemic hamsters, respectively. These fractions were also found efficient in increasing glucose uptake by L6 skeletal muscle cells but did not show any effect on cell viability of L6 skeletal muscle cells.

Conclusion: Based on the results, the present study revealed that ethyl acetate fraction of the fruits of X. granatum and X. moluccensis lowered blood glucose profile by increasing the glucose uptake by L-6 and this may be the possible mechanisms for the antidiabetic and antidyslipidemic action.

Keywords: Xylocarpus granatum, Xylocarpus moluccensis, Streptozotocin-induced diabetic rats, Antihyperglycemic activity, Antidyslipidemic activity, Glucose uptake assay

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Srivastava, S. P., N. Rahuja, S. Srivastava, A. K. Tamrakar, S. K. Mishra, S. Srivastava, V. Lakshmi, M. N. Srivastava, and A. K. Srivastava. “ANTIHYPERGLYCEMIC AND ANTIDYSLIPIDEMIC ACTIVITY IN ETHYL ACETATE FRACTION OF THE FRUITS OF XYLOCARPUS GRANATUM AND XYLOCARPUS MOLUCCENSIS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, 1, pp. 532-6, https://innovareacademics.in/journals/index.php/ijpps/article/view/4968.
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