A SIMPLE AND A CHEAP UV ASSAY METHOD DEVELOPMENT AND VALIDATION FOR THE ESTIMATION OF EPLERENONE IN TABLETS
Objective: To develop a simple and a cheap UV spectrophotometric assay method for the estimation of Eplerenone in tablets and validate as per ICH guidelines.
Methods: The optimized method uses 100% potassium dihydrogen orthophosphate, pH 2.0 as a solvent for the estimation of assay of Eplerenone in tablets at a detection wavelength of 245 nm.
Results: The developed method resulted in Eplerenone exhibiting linearity in the range 5-15Î¼g/ml. System precision and intra-day precision are exemplified by relative standard deviation of 1.17% and 0.94% respectively. Method was found to be rugged as precision was found to be 1.52%. Percentage Mean recovery was found to be in the range of 98â€102 by percentage method during accuracy studies.
Conclusion: A simple and a cheap UV spectrophotometric assay method were developed and validated for the estimation of Eplerenone in tablets as per ICH guidelines and hence it can be used for routine analysis in various pharmaceutical industries.
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