ANTICANCER AND ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF HIPPOPHAE SALICIFOLIA IN EAC INDUCED SWISS ALBINO MICE

  • Mainak Chakraborty Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India
  • Indrajit Karmakar Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India
  • Sagnik Haldar Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India
  • Abhimanyu Nepal Department of HC, HS & FW. Ayush, Singtam, Govt. of Sikkim, India
  • Pallab Kanti Haldar Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, West Bengal, India

Abstract

Objective: Anticancer activity of methanol extract of Hippophae salicifolia (MEHS) bark was evaluated using the Ehrlich Ascites Carcinoma (EAC) cells on Swiss albino mice.

Methods: In vitro cytotoxicity assay has been evaluated by using the trypan blue and MTT assay method. The determination of in vivo anticancer activity was performed using EAC cells (2 × 106) induced mice groups (n = 12). After treatment with MEHS at the doses of 50 and 100 mg/kg b. w. respectively for 9 days, half of the mice of each group were sacrificed and the rest were kept for increase in life span determination. The anticancer potential of MEHS was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters and biochemical estimations. Furthermore, tissue antioxidant parameters were checked from liver homogenate.

Results: In MEHS treated groups (50 and 100 mg/kg b. w.) tumor volume, tumor weight, viable cell count was significantly decreased as compared to that of the EAC control group. Life span increased by 34% and 43% in a dose dependant manner as compared to EAC control group. The hematological, biochemical and liver tissue antioxidant parameter are significantly (p < 0.05) restored towards the normal level after treatment with MEHS.

Conclusion: From the above study it can be concluded that the MEHS has significant anticancer activity in the dose dependent manner.

Keywords: Trypan blue, MTT, EAC, Antioxidant, Tumor

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Chakraborty, M., I. Karmakar, S. Haldar, A. Nepal, and P. K. Haldar. “ANTICANCER AND ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF HIPPOPHAE SALICIFOLIA IN EAC INDUCED SWISS ALBINO MICE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 8, June 2015, pp. 180-4, https://innovareacademics.in/journals/index.php/ijpps/article/view/6710.
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