PHYTOCHEMICAL COMPOSITION, ANTIOXIDANT AND IN-VITRO ANTI-INFLAMMATORY ACTIVITY OF ETHANOL EXTRACT OF RUTA GRAVEOLENS L. LEAVES

  • Giresha A. S.
  • Anitha M. G.
  • Dharmappa K. K. Department of studies in Biochemistry, Mangalore University, P. G centre, Chikka aluvara, Kodagu, India

Abstract

Objective: The uses of medicinal herbs in prevention and treatment of numerous diseases attracting attention of scientists worldwide. It is necessary to bring vital healthcare to the people and the better substitute for various infectious diseases. Ruta graveolens is traditionally used as a medicinal plant as well as a flavoring agent in food. In the present study R. graveolens leaves extracts were evaluated for phytochemical and anti-inflammatory activity.

Methods: The phytochemical, antioxidant (DPPH), ferrous reducing antioxidant property, Anti-lipid peroxidation and superoxide scavenging activity of extracts were assayed by spectrophotometerically. Anti-inflammatory activities; Human plural fluid phospholipase A2 (HPF-PLA2) inhibition by egg yolk plate method and lipoxigenase (LOX) inhibition were assayed by IN VITRO method.

Results: The phenolics were significantly higher than the flavonoids, saponins and alkaloids contents in all the extracts. Among extracts of R. graveolens, ethanol and water extracts exhibits more phytochemicals. The ethanol extract had the highest antioxidant activity followed by the aqueous extract. The ethanol extract shows highest DPPH free radical scavenging, ferric reducing, superoxide scavenging and anti-lipid peroxidation activity and IC50 was 3.27±0.03, 3.58±0.05, 3.87±0.04 and 4.77±0.04 µg/µl respectively at dose-dependent manner. Further ethanol and water extracts were subjected for LOX inhibition in the concentration dependent manner and IC50 values are 4.25±0.05, 5.15±0.05 µg/µl for 5-LOX and 4.15±0.04, 4.66±0.05 µg/µl for 15-LOX respectively.

Conclusion: The result shows R. graveolens has strong antioxidant property and anti-inflammatory activity. It suggests that the strong correlation between antioxidant activity and the phytochemical contents of the extracts.

Keywords: HPF, Human Pleural Fluid, Anti-inflammatory, sPLA2, Secretory Phospholipase A2, HPF-PLA2, Human Pleural Fluid-PLA2. LOX Lipoxigenase, Lipid peroxidation, Anti-inflammation.

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References

1. Bhau BS. Molecular markers in the improvement of the medicinal plants. Med Aromat Plants 2012;1:2.
2. Falodun A, Okunrobo LO, Uzoamaka N. Phytochemical screening and anti-inflammatory evaluation of methanolic and aqueous extracts of Euphorbia heterophylla Linn (Euphorbiaceae). Afr J Biotech 2006;5:529-34.
3. Okwu DE. Evaluation of the chemical composition of indigenous spices and flavouring agents. Global J Pure Appl Sci 2001;7:455-9.
4. Upadhyay RK. Plant latex: a natural source of pharmaceuticals and pesticides. Green Pharm 2011;5:169–80.
5. Pandey M, Debnath M, Gupta S, Chikara SK. Phytomedicine: an ancient approach turning into future potential source of therapeutics. J Pharm Phytother 2011;3:27–37.
6. Uhl W, Buchler M, Nevalainen TJ, Deller A, Beger HG. Serum phospholipase A2 in patients with multiple injuries. J Trauma 1990;30:1285-90.
7. Nevalainen TJ, Gronroos JM, Kortesuo PT. Pancreatic and synovial type phospholipases A2 in serum samples from patients with severe acute pancreatitis. Gout 1993;34:1133-6.
8. Murakami M, Taketomi Y, Sato H, Yamamoto K. Secreted phospholipase A2 revisited. J Biochem 2011;150:233-55
9. Holmes MV, Simon T, Exeter HJ, Folkersen L, Asselbergs FW, Guardiola M, et al. Secretory phospholipase A2-IIA and cardiovascular disease A mendelian randomization study. J Am Coll Cardiol 2013;62:1966–76.
10. Stafforini DM, Zimmerman GA. Unraveling the PAF-AH/lp-PLA2 controversy. J Lipid Res 2014;55:1811-14.
11. Goetano GD, Donati MD, Cerletti C. Prevention of thrombosis and vascular inflammation: benefits and limitations of selective or combined cox-1, cox-2 and 5-lox inhibitions. Trends Pharmacol Sci 2003;24:245-52.
12. Dinarello CA. Anti-inflammatory agents: present and future. Cell 2010;140:935–50.
13. Thimmegowda NR, Dharmappa KK, Kumar CS, Sadashiva MP, Sathish AD, Nanda BL, et al. Synthesis and evaluation of tricyclic dipyrido diazepinone derivatives as inhibitors of secretory phospholipase A2 with anti-inflammatory activity. Curr Top Med Chem 2007;7:811-20.
14. Sadashiva MP, Nataraju A, Mallesha H, Rajesh R, Vishwanath BS, Rangappa KS. Synthesis and evaluation of trimethoxyphenyl isoxazolidines as inhibitors of secretory phospholipase A2 with anti-inflammatory activity. Int J Mol Med 2005;16:895-904.
15. Oliva A, Meepagala KM, Wedge DE, Harries D, Hale AL, Aliotta G, et al. Natural fungicides from Ruta graveolens L. leaves including a new quinolone alkaloid. J Agric Food Chem 2003;51:890-96.
16. Ojala T, Remes S, Haansuu P, Vurela H, Hiltunen R, Haahtela K, et al. Antimicrobial activity of some coumarin containing herbal plants growing in Finland. J Ethnopharmacol 2000;73:299-305.
17. Raghav SK, Gupta B, Agrawal C, Goswami K, Das HR. Anti-inflammatory effect of R. graveolens L. in murine macrophage cells. J Ethnopharmacol 2006;104:234-9.
18. Preethi KC, Kuttan G, Kuttan R. Anti-tumour activity of ruta graveolens extract. Asian Pac J Can Prev 2006;7:439-43.
19. Ivanova A, Mikhova B, Najdenski H, Tsvetkova I, Kostova I. Antimicrobial and cytotoxic activity of ruta graveolens. Fitotera 2005;76:344-7.
20. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidative substrates by means of Folin-Ciocalteau reagent. Method Enzymol 1999;299:152.
21. Yusuf AZ, Zakir A, Shemau Z, Abdullahi M, Halima SA. Phytochemical analysis of the methanol leaves extract of Paullinia pinnata linn. J Pharm Phytother 2014;6:10-6.
22. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid content in mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64:555–9.
23. Jayaprakash GK, Singh RP, Sakariah KK. Antioxidant activity of grape seed extracts on peroxidation models in-vitro. J Agric Food Chem 2001;55:1018-22.
24. Dasgupta N, De B. Antioxidant activity of piper betel L. leaf extract in vitro. Food Chem 2004;88:219–24.
25. Blios MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199.
26. Buege JA, Aust SD. Microsomal lipid peroxidation. Method Enzymol 1978;52:302-10.
27. Viswanath BS, Fawzy AA, Franson RC. Edema inducing activity of phospholipase A2 purified from human synovial fluid and inhibition by aristolochic acid. Inflammation 1988;12:549-61.
28. Gutierrez JM, Avila C, Rojas E, Cerdas L. An alternative in vitro method for testing the potency of the polyvalent antivenom produced in costa rica. Toxicon 1988;26:411-3.
29. Aharony D, Stein RL. Kinetic mechanism of guinea pig neutrophil 5-lipoxygenase. J Biochem 1986;261:11512-9.
30. Axelrod B, Cheesbrough TM, Laakso S. Lipoxygenase from soybeans. Method Enzymol 1981;71:441-51.
31. Boman HG, Kaletta U. Chromatography of rattlesnake venom: a separation of three phosphodiesterases. Bioch Biophys Acta 1957;24:619-31.
32. Sun GY, Horrocks LA, Farooqui AA. The roles of NADPH oxidase and phospholipases A2 in oxidative and inflammatory responses in neurodegenerative diseases. J Neurochem 2007;103:1-16.
33. Somchit N, Rahman SA, Ahmad Z, Abdullah AS. Anti-ulcer and wound healing activity of Ruta graveolens. Orientat Pharm Exp Med 2003;3:147-50.
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How to Cite
S., G. A., A. M. G., and D. K. K. “PHYTOCHEMICAL COMPOSITION, ANTIOXIDANT AND IN-VITRO ANTI-INFLAMMATORY ACTIVITY OF ETHANOL EXTRACT OF RUTA GRAVEOLENS L. LEAVES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 11, Aug. 2015, pp. 272-6, https://innovareacademics.in/journals/index.php/ijpps/article/view/7754.
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