HUMAN SECRETORY PHOSPHOLIPASE A2 (sPLA2) INHIBITION BY AQUEOUS EXTRACT OF MACROTYLOMA UNIFLORUM (SEED) AS AN ANTI-INFLAMMATORY ACTIVITY
Objective: Macrotyloma uniflorum (Horse gram) is an important legume widely consumed in the tropical south Asian countries including India. The present investigation is the elucidation of anti-inflammatory activity of M. uniflorum as it has several medicinal properties. The M. uniflorum was evaluated for inhibition of human secretory phospholipase A2 (sPLA2) as a function of anti-inflammatory activity.
Methods: The total phenols, antioxidant (DPPH scavenging), Anti-lipid peroxidation, PLA2 inhibition and lipoxygenase (5 & 15-LOX) inhibition activity of aqueous extracts of M. uniflorum coat and pulp were assayed by in vitro method. The aqueous extract of M. uniflorum seed coat was subjected to inhibit PLA2 enzymes from human inflammatory fluids (Human Synovial Fluid and Human Pleural Fluid) and snake venoms (Naja naja and Vipera russllii) using C labeled E. coli by in vivo method. A further effect of substrate and calcium concentration on inhibition of VRV-PLA2 in presence and absence of M. uniflorum coat extract were assayed.
Results: Aqueous coat extract of M. uniflorum shows higher phenolics and biological activity and inhibited all sPLA2 enzymes in concentration dependent manner. The IC50 values are found to be in the range of 11.42-20.88Î¼g and IC50 values for 5-LOX and 15-LOX is 25.92Î¼g and 32.47Î¼g respectively. The extract effectively neutralized indirect hemolytic activity and showed similar potency in neutralizing the in vivo sPLA2 induced mouse paw edema.
Conclusion: These findings suggest that, the active compound/s in extracts of M. uniflorum individually or synergistically responsible for observed sPLA2 inhibition.
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