IN VIVO EVALUATION OF BUDESONIDE MICROSPHERES FOR COLON SPECIFIC DRUG DELIVERY
Objective: The aim of the present study was to evaluate the in vivo behaviour of budesonide microspheres for colon targeted delivery.
Methods: In vivo therapeutic effect was evaluated using trinitrobenzene sulfonic acid (TNBS) induced colitis in rats. The therapeutic effect was assessed by determining the damage score, clinical activity score, colon/body weight(C/B) ratio and myeloperoxidase (MPO) activity measurement. The data were compared with standard drug 5-aminosalicylic acid (5-ASA). The targeting efficiency of the formulation was assessed by X-ray studies on the rabbit.
Results: The study showed that oral administration of budesonide microspheres exerted an affirmative impact on the colonic ulcer healing by decreasing the area of ulceration, reducing the mass of colon by improving the symptoms of colitis. MPO activity decreased significantly after oral administration of microspheres. Histopathological studies carried out also confirmed the result. The X-ray studies revealed that the formulations were able to target the colon.
Conclusion: The in vivo study confirmed the ability of budesonide microspheres in targeting the colonic region.
Keywords: Budesonide, Colitis, Targeted delivery, Myeloperoxidase, Ulceration, Clinical activity score
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