• Pressy P. Prakasia Research Scholar, Department of Botany, University of Kerala, Thiruvanathapuram
  • Ashalatha S. Nair


Objective: The present investigation was undertaken to appraise the antioxidant properties and the total crude alkaloid extract of Glycosmis pentaphylla leaves.

Methods: Radical attenuating abilities of total crude alkaloid extract were ascertained by six in vitro assays such as 2, 2-diphenyl 1-picryl hydrazyl (DPPH·) radical scavenging assay, ferric reducing antioxidant power assay (FRAP), 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) radical scavenging assay, nitric oxide (NO) scavenging assay, hydroxyl radical (OH·) scavenging assay, and reducing power capacity assessment.

Results: The total crude alkaloid extract showed a considerable antioxidant potentiality in comparison with the standards as evidenced by the IC50 values obtained in the DPPH· (966.93 µg/ml), FRAP (510.81 µg/ml), ABTS•+(400.47 µg/ml), OH· (1805.28 µg/ml), NO (1426.50 µg/ml). In the reducing power assay, the total crude alkaloid extract showed a concentration dependent reducing power potential.

Conclusion: These results suggest the potential of Glycosmis pentaphylla as a curative against free-radical-associated oxidative damage diseases.

Keywords: Glycosmis pentaphylla, Antioxidant assay, Total crude alkaloid, IC50


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1. Halliwell B, Gutteridge JMC. The chemistry of free radicals and related reactive species’. In: Free radicals in biology and medicine. 4th edn. Oxford University Press: Oxford; 2007. p. 30-79.
2. Halliwell B. “Reactive species and antioxidants. Redox biology is a fundamental theme of aerobic life”. Plant Physiol 2006;141:312-22.
3. Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 2004;52:794-804.
4. Nawal Kishore Dubey. Plants as a source of natural antioxidants. 1st ed. United Kingdom: CABI publishers; 2014. p. 242.
5. Kulkarni SK, Dhir A. Berberine: a plant alkaloid with therapeutic potential for central nervous system disorders. Phytother Res 2010;24:317-24.
6. Richard Larson A. Naturally occurring antioxidants. United States: CRC Press LLC Lewis Publishers; 1997. p. 145.
7. Ramchoun M, Harnafi H, Alem C, Benlys M, Elrhaffari L, Amrani S. Study on antioxidant and hypolipidemic effects of polyphenol rich extract from Thymus vulgaris and Lavendula multifida. Pharmacognosy Res 2009;1:106-12.
8. Dash DK, Yeligar VC, Nayak SS, Ghosh T, Rajalingam D, Sengupta P, et al. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn.) R. Br. on paracetamol-induced hepatotoxicity in rats. Trop J Pharm Res 2007;6:755-65.
9. Yildirim A, Oktay M, Bulaloulu V. The antioxidant activity of the leaves of Cydonia vulgaris. Turk J Med Sci 2001;31:23-7.
10. Nagulendran K, Velavan S, Mahesh R, Begum VH. In vitro antioxidant activity and total polyphenolic content of Cyperus rotundus rhizomes. E J Chem 2007;4:440-9.
11. Kirtikar KR, Basu BD. Indian medicinal plants. Dehradun: International Book Publisher; 1993. p. 1621-2.
12. Ghani A. Medicinal plants of bangladesh. 2nd ed. Bangladesh: Asiatic society of Bangladesh; 2003. p. 1-17.
13. Jaya Raju N, Rao BG. Evaluation of hepatoprotective activity of Glycosmis pentaphylla roots against CCl4 induced acute liver injury in rats. Int J Pharm Pharm Sci 2010;4:81-6.
14. Gupta N, Agarwal M, Bhatia V, Jha SK, Dinesh J. In vitro antioxidant activity of crude extracts of the plant Glycosmis pentaphylla Correa. Int J Pharm Sci Rev 2011;6:159-62.
15. Gonzales, Maria Victoria M, Tolentino, Angelina G. Extraction and isolation of the alkaloids from the Samanea Saman (acacia) bark: its antiseptic potential. Int J Life Sci Biotechnol Pharma Res 2014;3:119-24.
16. Chang ST, Wu JH, Wang SY, Kang PL, Yang NS, Shyur LF. Antioxidant activity of extracts from Acacia confusa bark and heartwood. J Agric Food Chem 2001;49:3420-4.
17. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem 1996;239:70–6.
18. Roberta Re, Nicoletta Pellegrini, Anna Proteggente, Ananth Pannala, Min Yang, Catherine Rice-Evans. Antioxidant activity is applying an improved ABTS radical cation decolorization assay. Free Radical Biol Med 1999;26:1231-7.
19. Garrat DC. The quantitative analysis of drugs. 3rd ed. Japan: Springer; 1964. p. 456-8.
20. Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method: a simple ‘test tube’ assay for determination of rates constants for reactions of the hydroxyl radical. Anal Biochem 1987;165:215-24.
21. Oyaizu M. Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:307-15.
22. Zanolari B, Guilet D, Marston A, Queiroz EF, Paulo MQ, Hostettmann K. Tropane alkaloids from the bark of Erythroxylum vacciniifolium. J Nat Prod 2003;66:497-502.
23. Zhang X, Yea W, Zhaoa S, Che CT. Isoquinoline and isoindole alkaloids from Menispermum dauricum. Phytochem 2004;65:929-32.
24. Phillipson JD. Alkaloids. Pharm J 1983;102-3.
25. Gonzales, Maria Victoria M, Tolentino, Angelina G. Extraction and isolation of the alkaloids from the Samanea Saman (Acacia) bark: its antiseptic potential. Int J Sci Tech Res 2014;3:119-24.
26. Shahrear Ahmad, Amin MN, Ashik Mosaddik. Studies on moisture content, biomass yield (Crude Plant Extract) and alkaloid estimation of in vitro and field grown plants of Rauvolfia serpentine. Pak J Biol Sci 2002;5:416-8.
27. Manik Ghosh, Barij Sinha N, Julio A Seijas, M Pilar Vázquez-Tato, Xesús Feás, Optimization process for increasing the yield of crude alkaloid from Litsea polyantha JUSS. Bark. Sciforum.net/conference/ecsoc-18/2555. p. 1-5.[Last accessed on 05 sep. 2015].
28. Gan RY, Xu XR, Song FL, Kuang L, Li HB. Antioxidant activity and total phenolic content of medicinal plants associated with prevention and treatment of cardiovascular and cerebrovascular diseases. J Med Plants Res 2010;4:2438-44.
29. Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne HD. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 2006;19:669-75.
30. Houcine Benmehdi, Nadia Bounoua, Abdelillah Amrouche, Driss Lahcene, Abdelhak Maazouzi. The phytochemical study, antioxidant and antimicrobial activities of Euphorbia Resinifera L. Int Res J Pharm 2013;4:44-50.
31. Amran Howlader, Farhana Rizwan, Shapna Sultana, Mohammad Rakibur Rahman, Shams-Ud-Doha KM, Rumana Mowla, et al. Antimicrobial, antioxidant and cytotoxic effects of methanolic extracts of leaves and stems of Glycosmis pentaphylla (Retz.). J Appl Pharm Sci 2011;1:137-40.
32. Rackova L, Majekova M, Kostalova D, Stefek M. Antiradical and Antioxidant activities of alkaloids isolated from Mahonia aquifolium. Structural aspects. Bioorg Med Chem 2004;12:4709-15.
33. Yanxuan W, Fuhou L. Studies on the antioxidant activities of total alkaloids from the fruits of Evodia rutaecarpa (Juss) Benth. Adv Mat Res 2012;396-398:52-5.
34. Maiza Benabdesselam F, Khentache S, Bougoffa K, Chibane M, Adach S. Antioxidant activities of alkaloid extracts of two Algerian species of Fumaria: Fumaria capreolata and Fumaria bastardii. Rec Nat Prod 2007;1:28-35.
35. Diplock AT. Will the good fairies please prove to us that vitamin E lessens human degenerative disease? Free Radical Res 1997;27:511–32.
36. Szollosi R, Varga IS. Total antioxidant power in some species of Labiatae (Adaption of FRAP method). Acta Biol Szeg 2002;46:125-7.
37. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 1996;239:70-6.
38. Ebenezer T, Kadamban D, Shekhawat MS, Justin Packia Jacob S. Phytochemical evaluation and antioxidant activity of Glycosmis mauritiana (Lam.) tanaka leaf extract. Int J Pharm Res Rev 2013;2:16-23.
39. Pulido R, Bravo L, Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem 2000;48:3396-402.
40. Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 2003;23:1719-26.
41. Schafer FQ, Buettner GR. Redox environment of the cell as viewed through the redox state of the glutathione disulfide/gluthathione couple. Free Radical Biol Med 2001;30:1191-212.
42. Aoxue Luo, Yijun Fan, Aoshuang Luo. In vitro free radical scavenging activities of polysaccharides from Polygonum multiflorum Thunb. J Med Plants Res 2011;5:966-72.
43. Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. Free radical scavenging properties of wheat extracts. J Agric Food Chem 2002;50:1619-24.
44. Wang M, Li J, Rangarajan M, Shao Y, La Voie EJ, Huang T, et al. Antioxidative phenolic compounds from Sage (Salvia officinalis). J Agric Food Chem 1998;46:4869-73.
45. Aline Augusti Boligon, Michel Mansur Machado, Margareth Linde Athayde. Technical evaluation of antioxidant activity. J Med Chem 2014;4:517-22.
46. Palmer RMJ, Ferrige AG, Moncada S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature 1987;327:524-6.
47. Rees DD, Palmer RM, Moncada S. Role of endothelium-derived nitric oxide in the regulation of blood pressure. Proc Natl Acad Sci USA 1989;86:3375-8.
48. Bhatia L, Bishnoi H, Chauhan P, Kinja K, Shailesh S. In-vitro comparative antioxidant activity of ethanolic extracts of Glycosmis pentaphylla and Bauhinia variegata. Recent Res Sci Technol 2011;3:1-3.
49. Umamaheswari M, Chatterjee TK. In vitro antioxidant activities of the fractions of Coccinia Grandis L. leaf extract. Afr J Tradit Complementary Altern Med 2008;5:61-73.
50. Spencer JPE, Jenner A, Aruoma OI. Intense oxidative DNA damage promoted by L-DOPA and its metabolities, implications for neurodegenerative disease. FEBS Lett 1994;353:246-50.
51. Ajila CM, Naidu KA, Bhat SG, Prasad Rao UJS. Bioactive compounds of mango peel extract. Food Chem 2007;105:982-8.
52. Gulcin I, Oktay M, Kirecci E, Kufrevioglu I. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L) seed extract. Food Chem 2003;83:371-82.
53. Duh PD. Antioxidant activity of burdock (Arctium lappa Linne.): Its scavenging effect on free-radical and active oxygen. J Am Oil Chem Soc 1998;75:455-61.
54. Duh PD, Du PC, Yen GC. Action of methanolic extract of Mung beans hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage. Food Chem Toxicol 1999;37:1055-61.
55. Anandhan Vignesh, Purushothaman Rama, Govindhan Lakshmanan, Kandasamy Murugesan. Chemical composition and antioxidant activity of essential oil from Glycosmis pentaphylla. Int J Bot Res 2014;4:19-28.
56. Panduranga Murthy G, Chandrasekhar KB, Leelaja BC, Lokesh S. Antimicrobial and antioxidant activities of tribal medicine formulation (TMF) accomplished for wound related remedies in biligirirangana hill area of chamarajanagara district, Karnataka (India). Int J Pharm Chem 2015;5:261-76.
57. Jayanthi P, Lalitha P. Reducing power of the solvent extracts of Eichhornia crassipes (MART.) SOLMS. Int J Pharm Pharm Sci 2011;3:126-8.
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How to Cite
Prakasia, P. P., and A. S. Nair. “EVALUATION OF INVITRO ANTIOXIDANT POTENTIAL OF THE TOTAL CRUDE ALKALOID EXTRACT OF GLYCOSMIS PENTAPHYLLA (RETZ.) CORREA LEAVES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 3, Mar. 2016, pp. 85-91, https://innovareacademics.in/journals/index.php/ijpps/article/view/9412.
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