EVALUATION OF INVITRO ANTIOXIDANT POTENTIAL OF THE TOTAL CRUDE ALKALOID EXTRACT OF GLYCOSMIS PENTAPHYLLA (RETZ.) CORREA LEAVES

  • Pressy P. Prakasia Research Scholar, Department of Botany, University of Kerala, Thiruvanathapuram
  • Ashalatha S. Nair

Abstract

Objective: The present investigation was undertaken to appraise the antioxidant properties and the total crude alkaloid extract of Glycosmis pentaphylla leaves.

Methods: Radical attenuating abilities of total crude alkaloid extract were ascertained by six in vitro assays such as 2, 2-diphenyl 1-picryl hydrazyl (DPPH·) radical scavenging assay, ferric reducing antioxidant power assay (FRAP), 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) radical scavenging assay, nitric oxide (NO) scavenging assay, hydroxyl radical (OH·) scavenging assay, and reducing power capacity assessment.

Results: The total crude alkaloid extract showed a considerable antioxidant potentiality in comparison with the standards as evidenced by the IC50 values obtained in the DPPH· (966.93 µg/ml), FRAP (510.81 µg/ml), ABTS•+(400.47 µg/ml), OH· (1805.28 µg/ml), NO (1426.50 µg/ml). In the reducing power assay, the total crude alkaloid extract showed a concentration dependent reducing power potential.

Conclusion: These results suggest the potential of Glycosmis pentaphylla as a curative against free-radical-associated oxidative damage diseases.

Keywords: Glycosmis pentaphylla, Antioxidant assay, Total crude alkaloid, IC50

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Prakasia, P. P., and A. S. Nair. “EVALUATION OF INVITRO ANTIOXIDANT POTENTIAL OF THE TOTAL CRUDE ALKALOID EXTRACT OF GLYCOSMIS PENTAPHYLLA (RETZ.) CORREA LEAVES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 3, Mar. 2016, pp. 85-91, https://innovareacademics.in/journals/index.php/ijpps/article/view/9412.
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