BIODIVERSITY OF ANTIBIOTIC-PRODUCING SOIL BACTERIA FROM YOGYAKARTA SPECIAL PROVINCE INDONESIA
Objective: The present study aims to evaluate the diversity of antibiotic-producing soil bacteria from Java, Indonesia.
Methods: An agar diffusion method to select the active isolates against Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 was used. Furthermore, a combination approach consisting thin layer chromatography-bioautography (TLC-bioautography) analysis and restriction fragment length polymorphism (RFLP) analysis using HaeIII (BsuRI) restriction enzyme digestion were applied.
Results: Out of 19 isolates obtained, 12 isolates exhibited antibacterial activity and subjected further analysis. Based on the Rf value of active spots obtained by TLC-bioautography, the isolates were classified into three groups i.e. isolates with the Rf value of 0.2-0.3 such as KP13 on E. coli, isolates with the Rf value of 0.4-0.6 such as LP6, P301 and T25A on S. aureus as well as P301 on E. coli and isolates with the Rf value of 0.7-0.9 such as J3, J4, J5, J7, JS and TL and LP on S. aureus as well as J3, J4, J5, J7, JS, KP13 and TL on E. coli. Moreover, based on RFLP profile the isolates were assigned into three groups at the similarity level of 56 %. The isolate KP13 was assigned as Group A, whereas the isolates TL, LP6 and J4 were assigned as Group B and the isolates LP, P301, T25A, P302, JS, J7, J5 and J3 were assigned as Group C. Interestingly, the isolate KP13 was absolutely different from others pointed by zero of % similarity and might produce more than one antibacterial compounds.
Conclusion: The soil bacteria isolates are differentiated into three groups and the KP13 isolate shows zero % similarity to the other isolates.
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