• Jayashree V Vel's University
  • Bagyalakshmi S
  • Manjula Devi K
  • Richard Daniel D


Objective: To study the in vitro anti-inflammatory activity of 4-benzylpiperidine.
Methods: This study was conducted to evaluate the in vitro anti-inflammatory activity of 4-benzylpiperidine using in vitro models such as inhibition
of albumin denaturation and proteinase inhibitory activity.
Results: This study revealed the dose-dependent inhibition of protein denaturation and proteinase inhibitory activity by 4-benzylpiperidine.
Conclusion: In the present study, results indicate that the 4-benzylpiperidine possess anti-inflammatory properties. The drug inhibited the heat
induced albumin denaturation and proteinase inhibitory activity. It shows dose-dependent significant activity when compared with a standard drug.
Hence, this study gives an idea that the 4-benzylpiperidine can be used as a lead compound for designing a potent anti-inflammatory drug which can
be used to cure inflammation.
Keywords: Anti-inflammatory activity, 4-Benzylpiperidine, Protein denaturation, Proteinase inhibitory activity.

Author Biography

Jayashree V, Vel's University
Department of Pharmacology


1. Chaudhari MG, Joshi BB, Mistry KN. In vitro anti-diabetic and anti-
inflammatory activity of stem bark of Bauhinia purpurea. Bull Pharm
Med Sci 2013;1(2):139-50.
2. Gilham B, Papachristodoulou K, Thomas JH. Wills Biochemical Basis
of Medicine. 3
ed. Oxford: Butterworth-Heinemenn; 1997.
3. Lewis DA. Anti-inflammatory Drugs from Plants and Marine Sources.
Vol. 27. Basel: Bikhauser Verlag; 1989.
4. Cotran RS, Kumar V, Robbins SL. Pathologic Basis of Disease. 9
Philadelphia, PA: Saunders W.B Company; 1994.
5. Vane JR, Botting RM. New insights into the mode of action of antiinflammatory
drugs. Inflamm Res 1995;44:1-10.
6. Ravikiran AG, Elumalai M, Eswaraiah C, Naresh V. An annual review
of anti-inflammatory medicinal plants. Int J Phytother 2012;2(1):23-7.
7. Tripathi KD. Essentials of Medical Pharmacology. 6
ed. New Delhi:
Jaypee Brothers Medical Publishers Ltd.; 2008.
8. Hallas J, Lauritsen J, Villadsen HD, Gram LF. Nonsteroidal antiinflammatory
and upper gastrointestinal bleeding,

groups by excess risk estimates. Scand
J Gastroenterol
9. Negus SS, Baumann MH, Rothman RB, Mello NK, Blough BE.
Selective suppression of cocaine - Versus food-maintained responding
by monoamine releasers in rhesus monkeys: Benzylpiperazine, (+)
phenmetrazine, and 4-benzylpiperidine. J Pharmacol Exp Ther
10. Arai Y, Hamamichi N, Kinemuchi H. Time-dependent inhibition of
rat brain monoamine oxidase by an analogue of 1-methyl-4-phenyl1,2,3,6-tetrahydropyridine
Neurosci Lett 1986;70(2):255-60.
11. Allan PG, Wesley LA, Ernest ES, Chester JC. Bis-ammonium salts.
Unsymmetric derivatives of substituted pyridine bases that are potent and
selective hypotensive agents 1. J Am Chem Soc 1957;79(14):3805-38.
12. Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of antiinflammatory
of Ashwagandha:
in vitro.
J 2012;4(29):47-9.
13. Sangeetha M, Kousalya K, Lavanya R, Sowmya C, Chamundeeswari D,
Reddy CU. In-vitro anti-inflammatory and anti-arthritic activity of leaves
of Cleodendron inerme. Res J Pharm Biol Chem Sci 2011;2(1):822-7.
14. Sakat SS, Juvekar AR, Gambhire MN. In vitro antioxidant and
inflammatory activity of methanol extract of Oxalis corniculata Linn.
Int J Pharm Pharmacol Sci 2010;2(1):146-55.
15. Oyedepo OO, Femurewa AJ. Anti-protease and membrane stabilizing
activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and
Tetrapleura tetraptera. Int J Pharmacogn 1995;33:65-9.
16. Leelaprakash G, Dass SM. Invitro anti-inflammatory activity
of methanol extract of Enicostema axillare. Int J Drug Dev Res
17. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs
with serum proteins, especially with some biologically active proteins.
J Pharm Pharmacol 1968;20(3):169-73.
18. Grant NH, Album HE, Kryzanauskas C. Stabilization of serum albumin
by anti-inflammatory drugs. Biochem Pharmacol 1970;19(3):715-22.
19. Das SN, Chatterjee S. Long term toxicity study of ART-400. Indian
Indig Med 1995;16(2):117-23.
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How to Cite
V, J., B. S, M. D. K, and R. Daniel D. “IN VITRO ANTI-INFLAMMATORY ACTIVITY OF 4-BENZYLPIPERIDINE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 8, Oct. 2016, pp. 108-10, doi:10.22159/ajpcr.2016.v9s2.12623.
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