IN VITRO ANTI-INFLAMMATORY ACTIVITY OF 4-BENZYLPIPERIDINE
Objective: To study the in vitro anti-inflammatory activity of 4-benzylpiperidine.
Methods: This study was conducted to evaluate the in vitro anti-inflammatory activity of 4-benzylpiperidine using in vitro models such as inhibition
of albumin denaturation and proteinase inhibitory activity.
Results: This study revealed the dose-dependent inhibition of protein denaturation and proteinase inhibitory activity by 4-benzylpiperidine.
Conclusion: In the present study, results indicate that the 4-benzylpiperidine possess anti-inflammatory properties. The drug inhibited the heat
induced albumin denaturation and proteinase inhibitory activity. It shows dose-dependent significant activity when compared with a standard drug.
Hence, this study gives an idea that the 4-benzylpiperidine can be used as a lead compound for designing a potent anti-inflammatory drug which can
be used to cure inflammation.
Keywords: Anti-inflammatory activity, 4-Benzylpiperidine, Protein denaturation, Proteinase inhibitory activity.
1. Chaudhari MG, Joshi BB, Mistry KN. In vitro anti-diabetic and anti-
inflammatory activity of stem bark of Bauhinia purpurea. Bull Pharm
Med Sci 2013;1(2):139-50.
2. Gilham B, Papachristodoulou K, Thomas JH. Wills Biochemical Basis
of Medicine. 3
ed. Oxford: Butterworth-Heinemenn; 1997.
3. Lewis DA. Anti-inflammatory Drugs from Plants and Marine Sources.
Vol. 27. Basel: Bikhauser Verlag; 1989.
4. Cotran RS, Kumar V, Robbins SL. Pathologic Basis of Disease. 9
Philadelphia, PA: Saunders W.B Company; 1994.
5. Vane JR, Botting RM. New insights into the mode of action of antiinflammatory
drugs. Inflamm Res 1995;44:1-10.
6. Ravikiran AG, Elumalai M, Eswaraiah C, Naresh V. An annual review
of anti-inflammatory medicinal plants. Int J Phytother 2012;2(1):23-7.
7. Tripathi KD. Essentials of Medical Pharmacology. 6
ed. New Delhi:
Jaypee Brothers Medical Publishers Ltd.; 2008.
8. Hallas J, Lauritsen J, Villadsen HD, Gram LF. Nonsteroidal antiinflammatory
and upper gastrointestinal bleeding,
groups by excess risk estimates. Scand
9. Negus SS, Baumann MH, Rothman RB, Mello NK, Blough BE.
Selective suppression of cocaine - Versus food-maintained responding
by monoamine releasers in rhesus monkeys: Benzylpiperazine, (+)
phenmetrazine, and 4-benzylpiperidine. J Pharmacol Exp Ther
10. Arai Y, Hamamichi N, Kinemuchi H. Time-dependent inhibition of
rat brain monoamine oxidase by an analogue of 1-methyl-4-phenyl1,2,3,6-tetrahydropyridine
Neurosci Lett 1986;70(2):255-60.
11. Allan PG, Wesley LA, Ernest ES, Chester JC. Bis-ammonium salts.
Unsymmetric derivatives of substituted pyridine bases that are potent and
selective hypotensive agents 1. J Am Chem Soc 1957;79(14):3805-38.
12. Chandra S, Chatterjee P, Dey P, Bhattacharya S. Evaluation of antiinflammatory
13. Sangeetha M, Kousalya K, Lavanya R, Sowmya C, Chamundeeswari D,
Reddy CU. In-vitro anti-inflammatory and anti-arthritic activity of leaves
of Cleodendron inerme. Res J Pharm Biol Chem Sci 2011;2(1):822-7.
14. Sakat SS, Juvekar AR, Gambhire MN. In vitro antioxidant and
inflammatory activity of methanol extract of Oxalis corniculata Linn.
Int J Pharm Pharmacol Sci 2010;2(1):146-55.
15. Oyedepo OO, Femurewa AJ. Anti-protease and membrane stabilizing
activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and
Tetrapleura tetraptera. Int J Pharmacogn 1995;33:65-9.
16. Leelaprakash G, Dass SM. Invitro anti-inflammatory activity
of methanol extract of Enicostema axillare. Int J Drug Dev Res
17. Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs
with serum proteins, especially with some biologically active proteins.
J Pharm Pharmacol 1968;20(3):169-73.
18. Grant NH, Album HE, Kryzanauskas C. Stabilization of serum albumin
by anti-inflammatory drugs. Biochem Pharmacol 1970;19(3):715-22.
19. Das SN, Chatterjee S. Long term toxicity study of ART-400. Indian
Indig Med 1995;16(2):117-23.
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