Neelima Rathi, Keerthana Harwalkar, Jayashree V, Ashwani Sharma, Nagashree N Rao



 Objective: Rich endowment of traditional knowledge plays a critical role in health care, food security, culture, environment and development. Traditional knowledge is also widely used by the aromatic, flavoring, food and health industries. Plant-based traditional medicine has often been used to identify and fast-track the development of modern food and drugs. Plants synthesize such wide array of secondary metabolites that includes alkaloids, glucosinolates, terpenoids and phenylpropanoids. This study is aimed to review on indigenous and exotic medicinal plants containing an astounding food flavoring metabolite; 2-hydroxy-4-methoxybenzaldehyde (HMB).

Material and Method: The review focuses on the isomer of vanillin, 2-hydroxy-4-methoxybenzaldehyde, also called MBALD, HMB, or 2H4MB, a flavor compound that is generally found in the roots and rhizomes of medicinal plants. This food flavoring phenylpropenoid is one of the least investigated isomers of vanillin. HMB is known to exhibit a wide array of medicinal properties. Though the molecular role of these metabolites remains largely unknown, they are known to play a key role in plant-environment interactions/stress response/defence signaling and application in pharmaceutical and nutraceutical industries.

Discussion and Conclusion: One of the key secondary metabolites is the group of phenylproponoids synthesized either through shikimic acid pathway or the malonate/acetate pathway. Phenylalanine ammonia lyase (PAL) is the enzyme involved in phenylpropanoid pathway catalysing the deamination of phenylalanine to synthesize cinnamic acid that subsequently synthesizes variety of phenylpropanoid products. Inspite of its medicinal importance, the regulatory molecular mechanism underlying the biosynthetic pathway is largely unknown.


2-hydroxy-4-methoxybenzaldehyde, Vanillin, Secondary metabolites, Antioxidants, Phenylpropanoid.

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Zhou M, Memelink J. Jasmonate-responsive transcription factors regulating plant secondary metabolism. Biotechnol Adv 2016;34(4):441-9.

Burt S. Essential oils: Their antibacterial properties and potential applications in foods-a review. Int J Food Microbiol 2004;94(3):223-53.

Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils-a review. Food Chem Toxicol 2008;46(2):446-75.

Dudareva N, Negre F, Nagegowda DA, Orlova I. Plant volatiles: Recent advances and future perspectives. Crit Rev Plant Sci 2006;25:417-40.

Umesh TG. In vitro callus induction and antioxidant potential of Decalepis hamiltonii (Wight and Arn). Int J Pharm Pharm Sci 2014;6(6):452-6.

Tripathi P, Dubey NK. Exploitation of natural products as an alternative strategy to control Syzygium jambos against selected human pathogens. Int J Pharm Pharm Sci 2011;3(2):44-7.

Yu O, Jez JM. Nature’s assembly line: Biosynthesis of simple phenylpropanoids and polyketides. Plant J 2008;54(4):750-62.

Suryavanshi A, Agarwal A, Kaler A, Bihade U, Kaur J, Kulbhushan B, et al. Comparative studies on the antioxidant potential of vanillin-producing Saccharomyces boulardii extracts. Oxid Antioxid Med Sci 2013;2(3):201-9.

Brenes M, García A, García P, Rios JJ, Garrido A. Phenolic compounds in Spanish olive oils. J Agric Food Chem 1999;47(9):3535-40.

Mackie DM, Smith D, Aitkin RA. Guidebook to Organic Synthesis. Vol. 12. New York, USA: Longman; 1990.p. 103-19.

Yamamoto M. Yamamoto and Dung Vanilla, Japan Probe Aroma Research. Vol. 7; 2006. p. 258-80.

Douglass F, Patel S, Travis M, Winkel EE. Vanillin synthesis from 4-hydroxybenzaldehyde. J Chem Educ 2007;84(7):1158-62.

Tahir A. Synthesis of Vanillin, Valhalla. J Chem Educ 1999;12:24-46.

Gary M, Andrews J, Bratz W, Hanssen O, Perry D. Preparation of vanillin from eugenol and sawdust. J Chem Educ 1977;54(12):776-8.

Gross GG. Formation and reduction of intermediate acyladenylate by aryl-aldehyde. NADP oxidoreductase from Neurospora crassa. Eur J Biochem 1972;31(3):585-92.

Snell K. Synthesis modification of the Escherichia coli chromosome: Enhancing the biocatalyst conversion of glucose in to aromatic chemicals. J Am Chem Soc 1996;118(24):5605-14.

Falconnier B. Vanillin as a product of ferulic acid biotransformation by the white-rot fungus Pycnoporuscina barinus: Identification of metabolic pathway. J Bio Technol 1994;37:123-32.

Li T, Rosazza JP. Biocatalytic synthesis of vanillin. Appl Environ Microb 2000;66(2):684-7.

Ming H, Shi Y. La-containing sba-15/h2o2 systems for the microwave assisted oxidation of a lignin model phenolic monomer. Madras Cienc Y Tecnol 2010;12(3):181-8.

Burri J, Graf M, Lambelet P, Löliger J. Vanillin: More than a flavoring agent-a potent antioxidant. J Sci Food Agric 1989;48(1):49-56.

Kamat JP, Ghosh A, Devasagayam TP. Vanillin as an antioxidant in rat liver mitochondria: Inhibition of protein oxidation and lipid peroxidation induced by photosensitization. Mol Cell Biochem 2000;209(1-2):47-53.

Boonchird C, Flegel TW. In vitro antifungal activity of eugenol and vanillin against Candida albicans and Cryptococcus neoformans. Can J Microbiol 1982;28(11):1235-41.

Kumar R, Sharma PK, Mishra PS. A review on the vanillin derivatives showing various biological activities. Int J Pharm Tech Res 2012;4(1):1251-61.

Imanishi H, Sasaki YF, Matsumoto K, Watanabe M, Ohta T, Shirasu Y, et al. Suppression of 6-TG-resistant mutations in V79 cells and recessive spot formations in mice by vanillin. Mutat Res 1990;243(2):151-8.

Ohta T, Watanabe M, Shirasu Y, Inoue T. Post-replication repair and recombination in uvrA umuC strains of Escherichia coli are enhanced by vanillin, an antimutagenic compound. Mutat Res 1988;201(11):107-12.

Kundu A, Jawali N, Mitra A. Shikimate pathway modulates the elicitor-stimulated accumulation of fragrant 2-hydroxy-4-methoxybenzaldehyde in Hemidesmus indicus roots. Plant Physiol Biochem 2012;56:104-8.

Chandrasekhara MR, Murthy KS. A review on Decalepis hamiltonii Wight and Arn. J Med Plants Res 2013;7(41):3014-29.

Chidambara KN, Rajashekaran T, Giridhar P, Ravishankar GA. Antioxidant property of Decalepis hamiltonii Wright and Arn. Indian J Exp Bio 2006;44:832-7.

Mukonyi KW, Isaiah ON. 2-hydroxy-4-methoxybenzaldehyde: Aromatic taste modifying compound from Mondia whitei. Bull Chem Soc Ethiop 2001;15(2):137-41.

Mohana DC, Raveesha KA, Lokanath KM. Herbal remedies for the management of seed-borne fungal pathogens by an edible plant Decalepis hamiltonii (Wight and Arn). Arch Phytopathol Plant Prot 2008;41(1):38-49.

Nagarajan S, Rao LJ. Determination of 2-hydroxy-4-methoxy benzaldehyde in roots of Decalepis hamiltonii (Wight and Arn.) and Hemidesmus indicus R. Br. J AOAC Int 2003;86(3):564-7.

Prajapati DS, Purohit SS, Sharma AK, Kumar T. Handbook of Medicinal Plants: Complete Source Book. Vol. 6. Jodhpur: Agrobios; 2001. p. 548-62.

George J, Pereira J, Divakar S, Udaysankar K, Ravishankar GA. A Method for the Preparation of Active Fraction from the Root of Decalepis hamiltonii, Useful as Bioinsectiside. Indian Patent No. 1301/98; 1998.

Murti PB, Sheshadiri TR. A study of the chemical compounds of Decalepis hamiltonii. Proc Indian Acad Sci 1941;14:93-9.

Nagarajan S, Rao LJ, Gurudutt KN. Chemical composition of the volatile of Decalepis hamiltonii (Wright and Arn). Flavor Fragrance J 2001;16:27-9.

Vedavathy S. Decalepis hamiltonii Wight and Arn.-an endangered source of indigenous health drink. Nat Prod Radiance 2004;3:22-3.

Srivastava A, Harish RS, Shivanandappa T. Antioxidant activity of the roots of Decalepis hamiltonii (Wright and Arn). LWT Food Sci Tech 2013;39:1059-65.

Lin J, Opoku AR, Geheeb-Keller M, Hutchings AD, Terblanche SE, Jäger AK, et al. Preliminary screening of some traditional zulu medicinal plants for anti-inflammatory and anti-microbial activities. J Ethnopharmacol 1999;68(1-3):267-74.

Parekh J, Chanda S. In vitro antimicrobial activities of extract of Launaea procumbens Roxb. (Labiateae), Vitis vinifera (Vitaceae) and Cyperus rotundus (Cyperaceae). Afr J Biomed Res 2006;9:89-93.

Murugan S, Devi PU, Parameswari NK, Mani KR. Antimicrobial activity of postharvest fungal rotting of fruit and vegetables. Postharvest Biol Technol 2004;32:235-45.

Laxmi A, Siddhartha S, Archana M. Antimicrobial screening of methanol and aqueous extracts Swertia chirata. Int J Pharm Pharm Sci 2011;3(4):142-6.

Shahzad A, Sharma S, Siddiqui SA. Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers. Vol. 2. Switzerland: Springer International Publishing; 2016. p. 169-84.

Nadkarni AN. Indian Material Medica. 1st ed., Vol. 1. Bombay, India: Popular Book Depot; 1989. p. 619.

Alam MI, Audpy B, Gomes A. Viper venom neutralization by Indian medicinal plants (Hemidesmus indicus and Pluchea indica). Phytother Reson 1996;10:58-61.

Bopanna KN, Bhagyalakshmi N, Rathod SP, Balaraman R, Kannan J. Cell culture derived Hemidesmus indicus in the prevention of hypercholesterolemia in normal and hyperlipidemic rats. Indian J Pharmacol 1997;29:105-9.

Kumari KV, Nishteswar K. Phytochemical and clinical evaluation of Sariba (Hemidesmus indicus) on wound healing. Int Res J Pharm 2012;3(3):277-81.

Kokwaro JO. Medicinal Plants of East Africa. Nairobi: Kenya Literature Bureau; 1976.

Neuwinger HD. African traditional medicine. A Dictionary of Plant Use and Applications. Germany: Medpharm Scientific Publ.; 2000. p. 5.

Watcho P, Donfack MM, Zelefack F, Nguelefack TB, Wansi S, Ngoula F, et al. Effects of the hexane extract of Mondia whitei on the reproductive organs of male rat. Afr J Tradit Complement Altern Med 2005;2(3):302-11.

Watcho P, Kamtchouing P, Sokeng S, Moundipa PF, Tantchou J, Essame JL, et al. Reversible antispermatogenic and antifertility activities of Mondia whitei L. in male albino rat. Phytother Res 2001;15(1):26-9.

Watcho P, Kamtchouing P, Sokeng SD, Moundipa PF, Tantchou J, Essame JL, et al. Androgenic effect of Mondia whitei roots in male rats. Asian J Androl 2004;6(3):269-72.

Nihei K, Yamagiwa Y, Kamikawa T, Kubo I. 2-hydroxy-4-isopropylbenzaldehyde, a potent partial tyrosinase inhibitor. Bioorg Med Chem Lett 2004;14(3):681-3.

Patnam R, Kadali SS, Koumaglo KH, Roy R. A chlorinated coumarinolignan from the African medicinal plant, Mondia whitei. Phytochemistry 2005;66(6):683-6.

Afolayan AJ, Adebola PO. In vitro propagation: A biotechnological tool capable of solving the problem of medicinal plants decimation in South Africa. Afr J Biotech 2004;3:683-7.

Wei Y, Zhao L, Deng Y. Research advance on the chemical components of Periploca sepium and the pharmacological action. J Tianjin Univ Tradit Chin Med 2009;28:165-6.

Miyazawa M, Fujita T, Yamafuji C, Matsui M, Kasahara N, Takagi Y, et al. Chemical composition of volatile oil from the roots of Periploca sepium. J Oleo Sci 2004;53:511-3.

Shi Q, Ma Y, Qin H. Chemical components and insecticidal activity of essential oil in Periploca sepium root bark to Schizaphis graminum. Acta Bot Boreal Occident Sin 2006;26:620-3.

Wang J, Liu H, Zhao J, Gao H, Zhou L, Liu Z, et al. Antimicrobial and antioxidant activities of the root bark essential oil of Periploca sepium and its main component 2-hydroxy-4-methoxybenzaldehyde. Molecules 2010;15(8):5807-17.

Peters CR. Notes on the distribution and relative abundance of Sclerocarya birrea Hochst. (Anacardiaceae). Mono Syst Bot Mo Botan Gardens 1988;25:403-10.

Shackleton CM. Growth and fruit production of Sclerocarya birrea in the South African lowveld. Agrofore Syst 2002;55:175-80.

Shackleton CM, Dzerefos CM, Shackleton SE, Mathabela FR. The use and trade in indigenous edible fruits in the Bushbuckridge savanna region, South Africa. Ecol Food Nutr 2000;39:225-45.

Shackleton CM, Shackleton SE. Direct use values of savanna resources harvested from communal savannas in the Bushbuckridge lowveld, South Africa. J Trop Forest Prod 2000;6:21-40.

Mariod A, Abdelwahad S. Sclerocarya birrea (Marula), An African tree of nutritional and medicinal uses: A review. Food Rev Int 2012;28:375-88.

Aganga AA, Mosase KW. Tannin content, nutritive value and dry matter digestibility of Lonchocarpus capassa, Zizyphus mucronata, Sclerocarya birrea, Kirkia acuminate and Rhus lancea seeds. Anim Feed Sci Tech 2001;91(1-2):107-13.

Chakraborty D, Sircar D, Mitra A. Phenylalanine ammonia-lyase-mediated biosynthesis of 2-hydroxy-4-methoxybenzaldehyde in roots of Hemidesmus indicus. J Plant Physiol 2008;165(10):1033-40.

Christopher M, Fraser CC. The Phenylpropanoid Pathway in Arabidopsis. The Arabidopsis Book; 2011. p. 9. DOI: 10.1199/tab.0152.

Huang J, Gu M, Lai Z, Fan B, Shi K, Zhou YH, et al. Functional analysis of the Arabidopsis PAL gene family in plant growth, development, and response to environmental stress. Plant Physiol 2010;153(4):1526-38.

Herrmann KM, Weaver LM. The shikimate pathway. Annu Rev Plant

Physiol Plant Mol Biol 1999;50:473-503.

Knaggs AR. The biosynthesis of shikimate metabolites. Nat Prod Rep 2003;20(1):119-36.

Tzin V, Galili G. The biosynthetic pathways for shikimate and aromatic amino acids in Arabidopsis thaliana. Arabidopsis Book 2010;8:e0132.

Liu J, Osbourn A, Ma P. MYB transcription factors as regulators of phenylpropanoid metabolism in plants. Mol Plant 2015;8(5):689-708.

Zhang L, Chen J, Li Q, Chen W. Transcriptome-wide analysis of basic helix-loop-helix transcription factors in Isatis indigotica and their methyl jasmonate responsive expression profiling. Gene 2016;576:150-9.

Dong J, Wan G, Liang Z. Accumulation of salicylic acid-induced phenolic compounds and raised activities of secondary metabolic and antioxidative enzymes in Salvia miltiorrhiza cell culture. J Biotechnol 2010;148(2-3):99-104.

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2-hydroxy-4-methoxybenzaldehyde, Vanillin, Secondary metabolites, Antioxidants, Phenylpropanoid.





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Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 10 October 2017 Page: 105-110

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Authors & Affiliations

Neelima Rathi
Department of Biotechnology, R V College of Engineering, Bengaluru - 560 059, Karnataka, India.

Keerthana Harwalkar
Department of Biotechnology, R V College of Engineering, Bengaluru - 560 059, Karnataka, India.

Jayashree V
Department of Biotechnology, R V College of Engineering, Bengaluru - 560 059, Karnataka, India.

Ashwani Sharma
Department of Biotechnology, R V College of Engineering, Bengaluru - 560 059, Karnataka, India.

Nagashree N Rao
Department of Biotechnology, R V College of Engineering, Bengaluru - 560 059, Karnataka, India.

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