ISOLATION OF BIOACTIVE COMPOUND OF MICHELIA CHAMPACA L. BARK AND ITS ACTIVITY TEST USING MECHANISM-BASED YEAST BIOASSAY
DOI:
https://doi.org/10.22159/ajpcr.2016.v9i5.12856Abstract
Objectives: This study aimed to isolate the active compound of Michelia champaca L. bark and test its activity using mechanism-based yeast bioassay.
Methods: The bark was extracted by methanol; fractionation was done by liquid-liquid extraction (LLE) using n-hexane, ethyl acetate, and water. The
activity of LLE fractions was tested by mechanism-based yeast bioassay. The most active fraction was then separated by vacuum liquid chromatography,
further separated by classical column chromatography and purified by recrystallization. The isolate was characterized by ultraviolet-visible, infrared
spectrophotometric method, nuclear magnetic resonance spectroscopy, and mass spectrometric method.
Results: The isolation process resulted in an isolate named MCET51. Characterization data showed that MCET51 was proved as liriodenine (C17H9NO3) with molecular weight 275 (m/z), an aporphine alkaloid. The activity assay showed that liriodenine was active against Saccharomyces cerevisiae strain 1140, 1353, and 1138 with IC12 values were 22.15±1.71, 24.76±0.56, and 7.02±1.85 μg/ml, respectively.
Conclusions: It can be concluded that M. champaca L. bark contained liriodenine which was active both as topoisomerase I inhibitor and
topoisomerase II inhibitor.
Keywords: Michelia champaca L., Topoisomerase inhibitor, Mechanism-based yeast bioassay, Liriodenine.
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