CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF DITERPENE AND ESSENTIAL OILS OF Hedychium roxburghii BL. RHIZOME

  • Rika Hartati School of Pharmacy Bandung Institute of Technology
  • Asep Gana Suganda
  • Irda Fidrianny

Abstract

Objective: The objective of the present study were to isolate and determine diterpene compound and essential oils from H. roxburghii Bl. rhizome, and investigated those antimicrobial activity.

Methods: The essential oils was obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by GC/MS method.  Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-NMR, 13C-NMR, HSQC-NMR, HMBC-NMR and carbon coupling NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method.

Results: The oils exhibited antimicrobial activity against Bacillus subtilis ATCC 6633 (MIC 1,750 μg/ml), Staphylococcus aureus ATCC 6538 (MIC 1,750 μg/ml), Escherichia coli ATCC 8939 (MIC 3,500 μg/ml), Pseudomonas aeruginosa ATCC 9027 (> 3,500 μg/ml) and Candida albicans ATCC 10231 (MIC 875 μg/ml). A phytochemical study of the rhizome essential oils of H. roxburghii Bl. were performed by GC/MS and the result showed that fenchyl acetate (45.85%) was the main component of the oils. Compound 1 was identified as diterpene compound, coronarin E. Coronarin E have not exhibited MIC at 512 μg/ml, however it showed inhibition profile against all of tested microbes.

Conclusion: The essential oils and ethanolic residual-distillation extract of H. roxburghii Bl. rhizome exhibited weak antimicrobial profile. Compound 1 was identified as diterpene compound, (coronarin E), it was exhibited weak antimicrobial activity, but showed inhibition profile against all of tested microbes.

 Keywords: Hedychium roxburghii, Zingiberaceae, antimicrobial, essential oils, coronarin E

Author Biography

Rika Hartati, School of Pharmacy Bandung Institute of Technology
Pharmaceutical Biology Reasearch Group School of Pharmacy

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How to Cite
Hartati, R., A. G. Suganda, and I. Fidrianny. “CHEMICAL COMPOSITION AND ANTIMICROBIAL ACTIVITY OF DITERPENE AND ESSENTIAL OILS OF Hedychium Roxburghii BL. RHIZOME”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 8, no. 5, Sept. 2015, pp. 209-14, https://innovareacademics.in/journals/index.php/ajpcr/article/view/7348.
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