ANTIHYPERTENSIVE ACTIVITY OF EXTRACT AND FRACTIONS OF MATOA (POMETIA PINNATA J. R & G FORTS) LEAVES
Objective: The purpose of this study was to determine antihypertensive activity of extract and fractions of matoa (Pometia pinnata) leaves.
Methods: Matoa leaves were extracted by reflux, followed by evaporating using rotary evaporator. Hypertension was induced by 50 mg/kg bw. NaCl
and 1.5 mg/kg bw. prednisone orally, every day as long as 28 days, then continued over the next 28 days in the therapy period. Male Sprague Dawley
rats were divided into 12 groups which were hydrochlorothiazide (0.45 mg/kg bw.), control group hypertensive, control normal, matoa leaves extract
(MLE) (with doses of 50 mg/kg bw., 100 mg/kg bw., and 150 mg/kg bw.), ethylacetate fraction (with doses of 4.35 mg/kg bw., 8.71 mg/kg bw., and
13.06 mg/kg bw.), and water fraction (with doses of 10 mg/kg bw., 21.88 mg/kg bw., and 32.82 mg/kg bw.). Measurement of systolic and diastolic
blood pressure was done every weeks using direct tail-cuff of noninvasive method. Then histomorphology of muscle heart was performed at the end
of this research.
Results: Ethylacetate fraction of matoa leaves 13.08 mg/kg bw. and MLE 150 mg/kg bw. gave significant result in lowering blood pressure (p<0.05) on
the 28th day of therapy and showed an equal profile with hydrochlorothiazide (0.45 mg/kg bw). Histomorphological result of ratâ€™s muscle heart found
collagen production was increased in NaCl-prednisone induced rats.
Conclusions: Extract and fractions of P. pinnata leaves could decrease blood pressure of NaCl-prednisone induced hypertension rats, but this effect
was not liniear with doses and they did not decrease the collagen production in cardiac myocardium compared to normal group.
Keywords: Pometia pinnata, Leaves, Hypertension, Tail-cuff noninvasive method, Blood pressure.
1. Department of Health RI. Reports Results of Health Research in Indonesia on 2007. Jakarta: Research and Development of Health Centers-Department of Health RI; 2008.
2. World Health Organization. A Global Brief on Hypertension. Geneva: WHO Press; 2013.
3. National Heart, Lung and Blood Institute. The Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation and Treatment of High Blood Preasure (JNC 7). Bethesda: NIH Publication; 2003. p. 1-34.
4. Badyal DK, Lata H, Dadhich AP. Animal models of hypertension and effect of drugs. Indian J Pharmacol 2003;35:349-62.
5. Hattori T, Murase T, Iwase E, Takahashi K, Ohtake M, Tsuboi K, et al. Glucocorticoid-induced hypertension and cardiac injury: Effects of mineralocorticoid and glucocorticoid receptor antagonism. Nagoya J Med Sci 2013;75(1-2):81-92.
6. Sinha A, Bagga A. Pulse steroid therapy. Indian J Pediatr 2008;75:1057-66.
7. Doggrell SA, Brown L. Rat models of hypertension, cardiac hypertrophy and failure. Cardiovasc Res 1998;39(1):89-105.
8. Dahl LK. Possible role of salt intake in the development of essential hypertension. In: Pork KD, Cottier PT, editors. Essential Hypertension-An International Symposium. Berlin: Springer-Verlag; 1960. p. 53-65.
9. Brunton LL, Lazo JS, Parker KL. Godman and Gilmanâ€™s the Pharmacological Basis of Therapeutics. 11th ed. USA: The McGraw-Hill Companies; 2006.
10. Purwidyaningrum I, Sukandar EY, Fidrianny I. Diuretic activity of different organs of matoa (Pometia pinnata) extracts and its influence on potassium and sodium level. Int J Pharm Phytochem Res 2016;8(2):244-7.
11. Vogel G. Drug Discovery and Evaluation Pharmacological Assays. USA: Springer; 1997.
12. Yuliandra, Armenia A, Arifin H. Study of antihypertensive effects of plant rope princess (Cassytha filiformis L.) in rats induced hypertension prednisone and salt. Proceedings of the National Seminar on Recent Developments III Clinical Pharmacy and Science in 2013.
13. Joshi UH, Ganatra TH, Desai TR, Tirgar PR. Evaluation of antihypertensive activity of evolvulus alsinoides in adrenaline induced hypertensive rats. Int J Pharm Pharm Sci 2012;4(4):194-8.
14. Alamgeer T, Malik M, Mushtaq M, Khan J, Qayyum R, Khan AQ, et al. Evaluation of antihypertensive effect of aqueous methanol extract of Caralluma tuberculata N.E.Br in Sprauge Dawley rats. Trop J Pharm Res 2015;14(3):455-62.
15. Jena M, Jena J, Biswal SB, Mishra S, Pal A. Effect of Eclipta alba on fructose induced hypertension in albino rats. Int J Pharm Pharm Sci 2013;5(3):281-5.
16. Mohanty PK, Sowers JR, Thames MD. Effects of hydrochlorothiazide and diltiazem on reflex vasoconstriction in hypertension. Hyper AHA J 1989;10(35):35-42.
17. Lopez B, Gonzalez A, Varo N, Laviades C, Querejeta R, Diez J. Biochemical assessment of myocardial fibrosis in hypertensive heart disease. Hyper AHA J 2001;38(5):1222-5.
18. Suliska N, Sukandar EY. The effectivity of captopril, losartan and amlodipine on hypertension in rat model of gentamicin-induced renal failure. Int J Pharm Pharm Sci 20146(6):146-51.
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