THE ANTIRHEUMATIC ACTIVITY OF MUNTINGIA CALABURA L. LEAVES ETHANOL EXTRACT AND ITS FRACTION

Abstract

Objective: Rheumatoid arthritis is chronic inflammation disorder that can be treated with anti-inflammatory drug such as methylprednisolone. Several medicinal plants shave the potential to be used in arthritis therapy, one of which is Muntingia calabura L. The objective of this research was to study the antirheumatic activity of M. calabura leaves ethanol extract and its n-hexane, ethyl acetate, and water fractions in a rheumatoid arthritic rat model.

Methods: Rheumatoid was induced in rats by intraplantar injection of Complete Freund’s Adjuvant (CFA) into the right foot paw of Wistar rats. The paw volume was measured by a plethysmometer. The histology of right foot paw was carried out. For chronic CFA inflammation, induced paw edema assay was used, whereas chronic inflammation was assessed by CFA induced arthritis. The histopathology of synovial joints (paw) was carried out.

Result: The results of the study revealed that in CFA-induced arthritic rats, methylprednisolone, M. calabura ethanol extract, and its fractions were able to reduce inflammation. Histopathology examination also showed a reduction of in cartilage destruction, influx of inflammatory cells, pannus formation, fibrin deposition, and synovitis.

Conclusion: M. calabura L. n-hexane fraction showed a better anti-rheumatic activity in rats among other fractions.

Keywords: Muntingia calabura L. leaves, Ethanol extract, N-hexane fraction, Ethyl acetate fraction, Antirheumatic.

 

Author Biography

Jamilah Sarimanah, Bandung Institute of Technology (ITB)
Jamilah Sarimanah is a Lecturer of University Setia Budi Surakarta and also a forth-year student at the School of Pharmacy of Bandung Institute of Technology pursuing her Doctoral Degree. She received a bachelor's degree and master's degree at the University Setia Budi Surakarta.

References

1. Balakrishnan KP, Narayanaswamy N, Duraisamy A. Tyrosinase inhibition and anti-oxidant properties of Muntingia calabura extracts: In vitro studies, ITC R and D centre, Peenya industrial area phase I, Bangalore 560 058, India. Int J Pharm Bio Sci 2011;2(1):294-303.
2. Khan YM, Ramadan D, Mundasada SC, Kumar NS, Kashyap HR, Chikkanna D. In vitro antidiabetic activity of Muntingia calabura root proteins. Int J Pharm Pharm Sci 2015;4(10):1526-34.
3. Banu K, Kalaivani K. GSMS/Ms analysis of Muntingia calabura steam and its antimicrobial effect, Periyar University, Salem. Int J Pharm Pharm Sci 2015;4(8):???.
4. Lin FL, Chen JJ, Shih CD. Antinociceptive and anti-inflammatory activity of the water-soluble extracts from leaves of Muntingia calabura, department pharmacy, graduate. J Clin Exp Pharmacol 2006;2(4):439-42.
5. Siddiqua A, Premakumari KB, Sultana R, Vithya, Savitha. Antioxidant activityand estimation of total phenolic content of Muntingia calabura by colorimetry, Dayananda Sagar college of pharmacy, Kumaraswamy, lay out, Bangalore, India. Int J Chem Tech Res 2010;2(1):205-8.
6. Suralkar AA. Anti arthritis potensial of helianthus annuus in laboratory animals. Asian J Pharm Clin Res 2015;8(6):???.
7. Zakaria ZA, Fatimah CA, Iais AM, Zaiton H, Henie EF, Sulaiman MR, et al. The in vitro antibacterial activity of Muntingia calabura extracts. Int J Pharmacol 2006;2(4):439-42.
8. Zakaria ZA, Somchit MN, Sulaiman MR, Jais AM, Fatimah CA. Effects of various receptor antagonists, pH and enzymes on Muntingia calabura antinociception in mice. Res J Pharmacol 2008;2(3):31-7.
9. Zakaria ZA, Nor RN, Jais AM, Somcit MN, Ghani ZD, Kader AA, et al. Antinociceptive, anti-inflammatory and antipyretic effects of Muntingia calabura aqueous extract in animal models. J Nat Med 2008;62(2):179-87.
10. Zakaria ZA, Mohamed AM, Jamil NS, Rofiee MS, Hussain MK, Sulaiman MR, et al. In vitro antiproliferative and antioxidant activities of the extracts of Muntingia calabura leaves. Am J Chin Med 2011;39(1):183-200.
11. Zakaria ZA, Sulfian AS, Ramasang K, Ahmat N, Sulaiman NR, Arifah KA, et al. In vitro antimicrobial of Muntingia calabura extrac and fraction. Afr J Microbiol Res 2010;4(4):304-8
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Sarimanah, J., K. A. I, E. Y. Sukandar, and N. F. Kurniati. “THE ANTIRHEUMATIC ACTIVITY OF MUNTINGIA CALABURA L. LEAVES ETHANOL EXTRACT AND ITS FRACTION”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 1, Jan. 2017, pp. 84-86, doi:10.22159/ajpcr.2017.v10i1.14102.
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