THE STUDY OF ANTIFUNGAL ACTIVITY FROM INDIGENOUS PLANTS FROM INDONESIA: AN IN VITRO STUDY
Objective: This study aimed to analyze new agents from extracted Indo esiaherbs possessing antifungal activity with better safety profile againstCandida albicans, Microsporum gypseum, and Trichophyton mentagrophytes.
Methods: The screening of 11 herbs was performed to determine the highest antifungal activity. Minimum inhibitory concentration and minimum fungicidal concentration of selected extracts, rosemary (Rosmarinus officinalis) leaves, java turmeric (Curcuma xanthorrhiza) rhizome, and ginger (Zingiber officinale) rhizome were determined using microdilution and agar diffusion methods. Extracts were then combined to evaluate further activities. Selected extracts were fractionated using liquid-liquid extraction, analyzed by thin-layer chromatography-direct (TLC) bioautography.
Results: Ethanolic extract of rosemary (R. officinalis) leaves, java turmeric (C. xanthorrihza) rhizome, and ginger (Z. officinalis) rhizome in combination showed the highest activity and synergistic interaction against Trichophyton mentagrophytes. There were several components from fractions that actively inhibited corresponding fungi according to TLC bioautography method.
Conclusion: Ethanolic extracts of rosemary, java turmeric, and ginger had the highest antifungal potency, both as extract and in combination comparable to ketoconazole as reference drug. These selected extracts are potential to be used as new antifungal agents.
Keywords: Antifungal, Rosemary, Ginger, Java turmeric, In situ, Bioautography thin-layer chromatography-direct.
2.Kurniati CR. Ethiopathogenesis of dermatophytosis. Berkala Ilmu Kesehatan Kulit and Kelamin 2008:20;243-50.
3.Adiguna MS. Epidemiology of Dermatomycosis in Indonesia 2004. 3rd ed. Jakarta: Balai Penerbit FK UI; 2006.
4.Cragg GM, Newman DJ. Medicinals for the millennia: The historical record. Ann N Y Acad Sci 2001;953:3-25.
5. WHO. Quality Control methods for Medicinal Plant Materials. Geneva: WHO Library Cataloguing in Publication Data; 1998.
6. Power DA, Peggy JM. Manual of BBL Products and Laboratory Procedures. 6th ed. New York: Becton Dickinson and Co.; 1988.
7. Tereschuk ML, Riera MV, Castro GR, Abdala LR. Antimicrobial activity of flavonoids from leaves of Tagetes minuta. J Ethnopharmacol 1997;56(3):227-32.
8. NCCLS. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard. 2nd ed. USA: NCCLS Document M27-A2; 2002.
9. Santos DA, Barros ME, Hamdan JS. Established a method of inoculum preparation for susceptibility testing of Trichophyton rubrum and Trichophyton mentagrophytes. J Clin Microbiol 2006;44:98-101.
10. Rukayadi Y, Hwang JK. In vitro antimycotic activity of xanthorrhizol isolated from Curcuma xanthorrhiza Roxb. against opportunistic filamentous fungi. Phytother Res 2007;21(5):434-8.
11. Rand KH, Houck HJ, Brown P, Bennett D. Reproducibility of the microdilution checkerboard method for antibiotic synergy. Antimicrob Agents Chemother 1993;37(3):613-5.
12. Widodo GP. Isolation of Active Component and Its Mechanism of Action from Daun Babadotan (Ageratum conyzoides L.), Doctoral Dissertation, School of Pharmacy, ITB. Bandung: Institut Teknologi Bandung; 2008.
13. Gangwar KK, Deepali GR, Gangwar RS. Ethnomedicinal plant diversity in Kumaun himalaya of Uttarakhand, India. Nat Sci 2010;8(5):66-78.
14. Gairola S, Shariff NM, Bhatt A. Influence of climate change on production of secondary chemicals in high altitude medicinal plants: Issues needs immediate attention. J Med Plants Res 2010;4(18):1825-9.
15. Frankel EN, Huang SW, Aeschbach R, Prior E. Antioxidant activity of a rosemary extract and its constituents, carnosic acid, carnosol, and rosmarinic acid, in bulk oil and oil-in-water emulsion. J Agric Food Chem 1996;44(1):131-5.
16. Valera MC, Maekawa LE, Chung A, Cardoso FG, Oliveira LD, Oliveira CL, et al. The effect of sodium hypochlorite and ginger extract on microorganisms and endotoxins in endodontic treatment of infected root canals. General Dent 2013;62(3):25-9.
17. Azizi A, Aghayan S, Zaker S, Shakeri M, Entezari N, Lawaf S. In vitro effect of Zingiber officinale extract on growth of Streptococcus mutans and Streptococcus sanguinis. Int J Dent 2015;2015:489842.
18. Ebrahimzadeh Attari V, Ostadrahimi A, Asghari Jafarabadi M, Mehralizadeh S, Mahluji S. Changes of serum adipocytokines and body weight following Zingiber officinale supplementation in obese women: A RCT. Eur J Nutr 2016;55(6):2129-36.
19. Marx W, McKavanagh D, McCarthy AL, Bird R, Ried K, Chan A. The effect of Ginger (Zingiber officinale) on platelet aggregation: A systematic literature review. PLoS One 2015;10(10):e0141119.
20. Kazeem MI, Akanji MA, Yakubu MT. Amelioration of pancreatic and renal derangements in streptozotocin-induced diabetic rats by polyphenol extracts of Ginger (Zingiber officinale) rhizome. Pathophysiology 2015;22(4):203-9.
21. Wilson PB. Ginger (Zingiber officinale) as an analgesic and ergogenic aid in sport: A systemic review. J Strength Cond Res 2015;29(10):2980-95.
22. Cojocaru SI, Stan M, Stoian G, Dinischiotu A. Effects of Zingiber officinale roscoe fresh extract on amelanotic melanoma and normal skin fibroblasts. Rev Med Chir Soc Med Nat Iasi 2015;119(2):592-6.
23. Li Y, Tran VH, Duke CC, Roufogalis BD. Gingerols of Zingiber officinale enhance glucose uptake by increasing cell surface GLUT4 in cultured L6 myotubes. Planta Med 2012;78(14):1549-55.
24. Halim MR, Marina SM, Sabariah I, Roziahanim M. Standardization and Phytochemical studies of Curcuma xanthorrhiza Roxb. IJPPS 2012;4(3):606-10.
25. Kim AJ, Kim YO, Shim JS, Hwang JK. Immunostimulating activity of crude polysaccharide extract isolated from Curcuma xanthorrhiza Roxb. Biosci Biotechnol Biochem
26. Lorian V. Antibiotics in Laboratory Medicine. 5th ed. London: Williams and Wilkins; 2005.
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