PHYTOCHEMICAL CONTENT AND ANTIOXIDANT ACTIVITIES IN DIFFERENT ORGANS OF POMELO (CITRUS MAXIMA [BURM.] MERR.) USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL AND PHOSPHOMOLYBDENUM ASSAYS
Objectives: The aims of this research were to determine antioxidant activity from various organs extracts of pomelo using 2,2-diphenyl-1picrylhydrazyl
) of DPPH antioxidant activities and exhibitory
concentration 50% (EC
) of phosphomolybdenum capacity, and correlation between two antioxidant assays.
Methods: Extraction was carried out by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant
activities using DPPH and phosphomolybdenum assays, determination of total phenolic, flavonoid, and carotenoid content were conducted by UVvisible
and its correlation
Results: The lowest IC
of DPPH and EC
of phosphomolybdenum was analyzed by Pearson's method.
of DPPH scavenging activity was shown by ethyl acetate cortex extract of pomelo (0.68 Âµg/ml), whereas the lowest EC
phosphomolybdenum capacity was given by ethyl acetate leaves extract of pomelo (101.36 Âµg/ml). Ethyl acetate cortex extract of pomelo had the
highest total phenolic content and ethyl acetate leaves extract had the highest total flavonoid content (TFC). There was a negative and significant
correlation between TFC in cortex and peel extracts of pomelo with their IC
Conclusions: All organs extracts of pomelo (except n-hexane peel extract) were classified as a very strong antioxidant by DPPH method. Flavonoid
compounds in cortex and peel extract of pomelo were the major contributor in antioxidant activities by DPPH method. DPPH and phosphomolybdenum
assays gave no linear results in antioxidant activities of leaves, cortex, and peel extracts of pomelo.
Keywords: Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Phosphomolybdenum, Pomelo, Three organs.
1. Gramza-Michalowska A, Czlapka-Matyasik M. Evaluation of the
antiradical potential of fruit and vegetable snacks. Acta Sci Pol Technol
2. Sharma P, Jha AB, Dubey RS, Pessarakli M. Reactive oxygen species,
oxidative damage, and antioxidative defense mechanism in plants
under stressful conditions. J Bot 2012;2012:1-26.
3. Apak R, Gorinstein S, BÃ¶hm VK, Schaich MK. Methods of
measurement and evaluation of natural antioxidant capacity/activity:
IUPAC technical report. Pure Appl Chem 2013;85:957-98.
4. Mohan M, Jaiswal BS, Kasture S. Effect of Solanum torvum on blood
pressure and metabolic alterations in fructose hypertensive rats.
J Ethnopharmacol 2009;126(1):86-9.
5. Khatua S, Roy T, Acharya K. Antioxidant and free radical scavenging
capacity of phenolic extract from Russula laurocerasi. Asian J Pharm
Clin Res 2013;6(4):156-60.
6. Xu BJ, Chang SK. A comparative study on phenolic profiles and
antioxidant activities of legumes as affected by extraction solvents.
J Food Sci 2007;72(2):S159-66.
7. Xu BJ, Chang SK. Total phenolic content and antioxidant properties of
eclipse black beans (Phaseolus vulgaris L.) as affected by processing
methods. J Food Sci 2008;73(2):H19-27.
8. Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S,
Bianchi M, et al. Total antioxidant capacity of plant foods, beverages
and oils consumed in Italy assessed by three different in vitro assays.
J Nutr 2003;133(9):2812-9.
9. Thaipong K, Boonprakob U, Crosby K, Zevallos LC, Byrne DH.
Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating
antioxidant activity from guava fruit extracts. J Food Comp Anal
10. Ordonez AA, Gomez JD, Vattuane MA, Isla MI. Antioxidant activities
of Sechium edule (Jacq.) Swartz extracts. Food Chem 2006;97:452-8.
11. Win M, Hamid A, Baharin B, Anwar FS, Pak-Dek MS. Phenolic
compounds and antioxidant activity of peanutâ€™s skin, hull, raw kernel
and roasted kernel flour. Pak J Bot 2011;43(3):1635-42.
12. Fidrianny I, Darmawati A, Sukrasno. Antioxidant capacities from
different polarities extracts of Cucurbitaceae leaves using FRAP, DPPH
assays and correlation with phenolic, flavonoid, carotenoid content. Int
J Pharm Pharm Sci 2014;6(2):858-62.
13. Apak R, Kubilay G, Birsen D, Mustava O, Saliha EC, Burcu B, et al.
Comparative evaluation of different polarities total antioxidant capacity
assays applied to phenolic compounds with the CUPRAC assay.
14. Fidrianny I, Amaliah A, Sukrasno. Antioxidant activities evaluation of
citrus leaves extracts from West Java-Indonesia using DPPH and FRAP
assays. Int J Pharmacog Phytochem Res 2016;8(4):611-8.
15. Prasad MP, Rajkumar RA. In vitro antioxidant assay of citrus species
using DPPH method. Indian J Adv Plant Res 2014;1(4):1-3.
16. Mashkor IM. Phenolic content and antioxidant activity of fenugreek
seeds extract. Int J Pharmacogn Phytochem Res 2014;6(4):841-4.
17. Blois MS. Antioxidant determination by the use of stable free radicals.
18. Bedawey AA. Characteristics of Antioxidant Isolated from Some Plants
Sources. Cairo: Shibin El-Kom; 2010. p. 1-11.
19. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of
antioxidant capacity through the formation of a phosphomolybdenum
Asian J Pharm Clin Res, Vol 9, Suppl. 2, 2016, 185-190
Fidrianny et al.
complex: Specific application to the determination of Vitamin E. Anal
20. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity,
phenol and flavonoid content of some selected Iranian medicinal plants.
Afr J Biotechnol 2006;5(11):1142-5.
21. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid
content in propolis by two complementary colorimetric methods.
J Food Drug Anal 2002;10:178-82.
22. Li XC, Wang XZ, Chen DF, Chen SZ. Antioxidant activity and
mechanism of protochatechuic acid in vitro. J Funct Food Health Dis
23. Crouch SR, Malmstadt HV. A mechanistic investigation of
molybdenum blue method for determination of phosphate. Analyt
24. Fidrianny I, Harnovi M, Insanu M. Evaluation of antioxidant activities
from various extracts of sweet orange peels using DPPH. Asian J Pharm
Clin Res 2014;7(3):186-90.
25. Ghasemi K, Ghasemi Y, Ebrahimzadeh MA. Antioxidant activity,
phenol and flavonoid contents of 13 citrus species peels and tissues.
Pak J Pharm Sci 2009;22(3):277-81.
26. Fidrianny I, Johan Y, Sukrasno. Antioxidant activities of different
polarity extracts from three organs of makrut lime (Citrus hystrix DC)
and correlation with total flavonoid, phenolic, carotenoid content.
Asian J Pharm Clin Res 2015;8(4):239-43.
27. Toh JJ, Khoo HE, Azrina A. Comparison of antioxidant properties
of pomelo (Citrus grandis (L) Osbeck) varieties. Int Food Res J
28. Duan L, Guo L, Dou LL, Yu KY, Liu EH, Li P. Comparison of
chemical profiling and antioxidant activities of fruits, leaves, branches,
and flowers of Citrus grandis Tomentosaâ€. J Agric Food Chem
29. Pichaiyongvongdee S, Rattanapun B, Haruenki R. Total polyphenol
content and antioxidant properties in different tissues of seven
pomelo (Citrus grandis (L.) Osbeck) cultivars. Kasetsart J Nat Sci
30. Ghafar MFA, Prasad KN, Weng KK, Ismail A. Flavonoid, hesperidine,
total phenolic contents and antioxidant activities from Citrus species.
Afr J Biotechnol 2010;9(3):326-30.
31. Hayat K, Hussain S, Abbas S, Farooq U, Ding B, Xia S, et al. Optimized
microwave-assisted extraction of phenolic acids from citrus mandarin
peels and evaluation of antioxidant activity in vitro. Sep Purif Technol
32. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants:
Chemistry, metabolism and structure-activity relationships. J Nutr
33. Foote CS. Free Radicals in Biology. 3
ed. New York: Academic Press;
34. Beutner S, Bloedorn B, Hoffmann T, Martin HD. Synthetic singlet
oxygen quenchers. Methods Enzymol 2000;319:226-41.
35. Charles DJ. Antioxidant Properties of Spices Shells and Other. London:
John Willey; 2013.
36. Kobayashi M, Sakamoto Y. Singlet oxygen quenching ability of
astaxanthin esters from the green alga Haematococcus pluvialis.
Biotechnol Lett 1999;21:265-9.
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