PHYTOCHEMICAL CONTENT AND ANTIOXIDANT POTENTIAL OF DIFFERENT ORGANS OF EGGPLANT (SOLANUM MELONGENA L.) GROWN IN WEST JAVA-INDONESIA


Irda Fidrianny, Siti Winarsih, Komar Ruslan

Abstract


 

 Objectives: The goals of this research were to evaluate antioxidant potential from different organs of eggplant (Solanum melongena L.) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50% (IC50) of DPPH, and exhibitory concentration 50% (EC50) of FRAP.

Materials and Methods: Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and FRAP assays, determination of total phenolic and flavonoid content were carried out by ultraviolet-visible spectrophotometry and correlation with their IC50 of DPPH and EC50 of FRAP capacities were analyzed by Pearson’s method.

Results: The lowest IC50 of DPPH scavenging activity 1.14 μg/ml and the lowest EC50 of FRAP capacity 49.80 μg/ml was given by ethanolic leaves extract of eggplant. Ethanolic leaves extract of eggplant also presented the highest total phenolic content (TPC) (8.87 g gallic acid equivalent/100 g), while the highest total flavonoid content was shown by ethyl acetate leaves extract (24.50 g quercetin equivalent/100 g). There was a significantly negative correlation between TPC in leaves and fruit extracts of eggplant with their IC50 of DPPH and EC50 of FRAP.

Conclusions: All different extracts of eggplant organs (except n-hexane stem extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in eggplant leaves and fruit extracts were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP showed linear results in antioxidant activities of eggplant leaves, fruit and stem extracts.


Keywords


Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Eggplant, Solanum melongena, Organs.

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References


Hodnick WF, Milosavljevic EB, Nelson JH, Pardini RS. Electrochemistry of flavonoids. Relationships between redox potentials, inhibition of mitochondrial respiration, and production of oxygen radicals by flavonoids. Biochem Pharmacol 1988;37(13):2607-11.

Mokbel MS, Hashinaga F. Antibacterial and antioxidant activities of banana (Musa, AAA CV. Cavendish) fruits peel. Am J Biochem Biotechnol 2005;1(3):125-31.

Sebei K, Gnouma A, Herchi W, Sakouhi F, Boukhchina S. Lipids, proteins, phenolic composition, antioxidant and antibacterial activities of seeds of peanuts (Arachis hypogaea l) cultivated in Tunisia. Biol Res 2013;46(3):257-63.

Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, et al. Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. J Nutr 2003;133(9):2812-9.

Zielinski AA, Haminiuk CW, Alberti A, Nogueira A, Demiate IM, Granato D. A comparative study of the phenolic compounds and the in vitro antioxidant activity of different Brazilian teas using multivariate statistical techniques. Food Res Int 2014;60:246-54.

Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid content of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5(11):1142-5.

Saleh GS. Chemical detection of some active compounds in egg plant (Solanum melongena) callus as compared with fruit and root contents. Int J Curr Microbiol Appl Sci 2015;4(5):160-5.

Thaipong K, Boonprakob U, Crosby K, Zevallos LC, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compost Anal 2006;19:669-75.

Arya N, Prakash OM, Verma AK, Vivekanand, Pant AK. Variation in antioxidant potential of Curcuma longa L. Collected from different ecological niches of western Himalayan region. Int J Pharm Pharm Sci 2015;7(7):85-90.

Settharaksa S, Madaka F, Chakree K, Charoenchai L. Total phenolic and flavonoid contents and antioxidant properties of Thai traditional herbal. Int J Pharm Pharm Sci 2014;6(9):564-6.

Blois MS. Antioxidant determination by the use of stable free radicals. Nature 1958;181:1199-2000.

Bedawey AA. Characteristics of Antioxidant Isolated from Some Plants Sources. Shibin El-Kom: Cairo; 2010. p. 1-11.

Benzi IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Anal Biochem 1996;239:70-6.

Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.

Kandoliya UK, Bajaniya VK, Bhadja NK, Bodar NP, Golakiya BA. Antioxidant and nutritional components of egg plant (Solanum melongena L.) fruit grown in Saurastra region. Int J Curr Microbiol Appl Sci 2015;4(2):806-13.

Piao XM, Chung JW, Lee GA, Lee JR, Cho GT, Lee HS, et al. Variation in antioxidant activity and flavonoid aglycones in eggplant (Solanum melongena L.) germplasm. Plant Breed Biotechnol 2014;2(4):396-403.

Apak R, Gorinstein S, Böhm VK, Schaich MK. Methods of measurement and evaluation of natural antioxidant capacity/activity: IUPAC technical report. Pure Appl Chem 2013;85:957-98.

Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 2005;53:4290-302.

Li XC, Wang XZ, Chen DF, Chen SZ. Antioxidant activity and mechanism of protochatechuic acid in vitro. J Funct Food Health Dis 2011;1:232-44.

Fidrianny I, Johan Y, Sukrasno. Antioxidant activities of different polarity extracts from three organs of makrut lime (Citrus hystrix DC) and correlation with total flavonoid, phenolic, carotenoid content. Asian J Pharm Clin Res 2015;8(4):239-43.

Somawathi KM, Rizliya V, Wijesinghe DG, Madhujith WM. Antioxidant activity and total phenolic content of different skin coloured brinjal (Solanum melongena). Trop Agric Res 2014;26:152-61.

Jung EJ, Bae MS, Jo EK, Jo YH, Lee SC. Antioxidant activity of different parts of eggplant. J Med Plants Res 2011;5(18):4610-5.

Sultana B, Hussain Z, Hameed M, Mushtaq M. Antioxidant activity among different parts of aubergine (Solanum melongena L.) Pak J Bot 2013;45(4):1443-8.

Barreira JC, Ferreira IC, Oliveira MB, Pereira JA. Effects of different phenols extraction conditions on antioxidant activity of almond (Prunus dulcis) fruits. J Food Biochem 2009;33:763-76.

Miliauskas G, Venskutonis PR, van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004;85:231-7.

Wicks LK, Wood LG, Garg ML. Methodology for the determination of biological antioxidant capacity in vitro: A review. J Sci Food Agric 2006;86:2046-56.

Nithiyanantham S, Varadharajan S, Siddhuraju P. Differential effects of processing methods on total phenolic content, antioxidant and antimicrobial activities of three species of Solanum. J Food Drug Anal 2012;20(4):844-54.

Loganayaki N, Siddhuraju P, Manian S. Antioxidant activity of two traditional Indian vegetables: Solanum nigrum L. and Solanum torvum L. Food Sci Biotechnol 2010;19:121-7.

Amic D, Amic DD, Beslo D, Rastija V, Lucic B, Trinajstic N. SAR and QSAR of the antioxidant activity of flavonoids. Curr Med Chem2007;14:827-45.

Padmashree A, Sharma GK, Semwal AD, Mahesh C. Antioxygenic activity of Solanum nigrum L. Leaves in sun flower oil model system and its thermal stability. Food Nutr Sci 2014;5:1022-9.

Akanitapichat P, Phraibung K, Nuchklang K, Prompitakkul S. Antioxidant and hepatoprotective activities of five eggplant varieties. Food Chem Toxicol 2010;48(10):3017-21.

Mongkolsilp S, Pongbupakit I, Sae-Lee N, Sitthithaworn W. Radical scavenging activity and total phenolic content of medicinal plants used in primary health care. SWU J Pharm Sci 2004;9(1):32-5.

Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J Nutr Biochem 2002;13:572-84.

Sroka Z, Cisowski W. Hydrogen peroxide scavenging, antioxidant and anti-radical activity of some phenolic acids. Food Chem Toxicol 2003;41:753-8.




About this article

Title

PHYTOCHEMICAL CONTENT AND ANTIOXIDANT POTENTIAL OF DIFFERENT ORGANS OF EGGPLANT (SOLANUM MELONGENA L.) GROWN IN WEST JAVA-INDONESIA

Keywords

Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Ferric reducing antioxidant power, Eggplant, Solanum melongena, Organs.

DOI

10.22159/ajpcr.2017.v10i8.18584

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 8 August 2017 Page: 144-149

Print ISSN

0974-2441

Online ISSN

2455-3891

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Authors & Affiliations

Irda Fidrianny
Department of Pharmaceutical Biology, Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
Indonesia

Siti Winarsih
Department of Pharmaceutical Biology, Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
Indonesia

Komar Ruslan
Department of Pharmaceutical Biology, Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
Indonesia


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