ANTICONVULSANT ACTIVITY OF CITRUS MAXIMUS LEAVES IN EXPERIMENTAL ANIMAL MODELS
Objective: To assess the anticonvulsant activity of ethanolic extract of Citrus maximus (EECM) leaves of maximal electroshock seizure (MES) and
pentylenetetrazol (PTZ)-induced seizure models on albino (Wistar strain) rats and mice.
Methods: Anticonvulsant activity was carried out by MES model and PTZ-induced clonic convulsions model; in each model, albino rats (Wistar strain)
of either sex were taken and divided into five groups, each consisting of 6 rats. One group was used as control (3% w/v gum acacia), one as standard
(phenytoin), and three groups for the test drug of EECM leaves (doses of 50, 100, and 200 mg/kg) treatment. The reduction in time or abolition of tonic
extensor phase of MES-convulsions was recorded for all the animals. In PTZ model, either delay or complete abolition of convulsions in rats treated
with diazepam and EECM leaves was noted for all the animals.
Result: EECM leaves reduced the extensor phase of convulsion in MES in a dose-dependent manner and decrease in the duration of convulsions in PTZ
model with increasing dose. Anticonvulsant activity was seen maximum at the dose of 200 mg/kg.
Conclusions: Thus, from the above two seizure models of MES and PTZ, it can be concluded that EECM leaves have got an anticonvulsant effect in an
increasing dose-dependent manner.
Keywords: Anticonvulsant, Citrus maximus, Maximal electroshock seizure, Pentylenetetrazol.
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