• Poonam Sangwan GDC Memorial College, Bhiwani
  • Harish Kumar



Objective: This paper consists of synthesis of chromium oxide (Cr2O3) nanoparticles by sol-gel technique, their characterization and investigation of antibacterial activity of these nanoparticles against pathogenic bacteria by measuring zone of inhibition, colony forming units and optical density on solid agar media as well as in liquid medium.

Method: The Cr2O3 nanoparticles were synthesized by sol-gel technique using TEOS as precursor. The synthesized chromium oxide nanoparticles were characterized by X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, UV-Visible spectroscopy and transmission electron microscopy (TEM) techniques. The antibacterial effect of these chromium oxide nanoparticles against Klebsiella pneumoniae was investigated both on the solid agar plates and in liquid medium supplemented with different concentrations of Cr2O3 nanoparticles. The antibacterial activity of chromium oxide nanoparticles was also compared with the antibacterial activities of the standard antibiotics such as ampicillin, chloramphenicol, penicillin G, streptomycin, sulphatriad and tetracycline which were taken in the form of hexa discs.

Results: Average particle size of the Cr2O3 nanoparticles was found to be 24.0 nm. It was observed that Klebsiella pneumoniae is resistant to the penicillin G and ampicillin but Cr2O3 nanoparticles shows good antibacterial property. The minimum inhibitory concentration (MIC) of Cr2O3 for Klebsiella pneumoniae is 2.5 mg/ml. The bacterial growth was monitored by measuring zone of Inhibition, colony formation unit (CFU) and optical density (OD) method.

Conclusion: Sol-gel technique is a convenient and easy technique for the synthesis of metal nanoparticles. Nanosized Cr2O3 particles showed an effective antibacterial activity against Klebsiella pneumoniae. Therefore, chromium oxide nanoparticles due to its low manufacturing cost and high effectiveness in antimicrobial properties may find wide applications in various industries to address safety issues. 


Download data is not yet available.


Yang X, Peng X, Xu C, Wang F. Electrochemical assembly

of Ni-xCr-yAL nanocomposites with excellent high-temperature

oxidation resistance. J Electrochem Soc 2009;156(7):167-75.

Li CL, Zhao HX, Takahashi T, Matsumura M. Improvement of corrosion resistance of materials coated with a Cr2O/NiCr delayer using a sealingtreatment. Mater Sci Eng A 2001;308(1-2):268-76.3

Al-Saadi TM, Hameed NA. Synthesis and structural characterization

of Cr2O3 nanoparticles prepared by using Cr (NO3) O and

triethanolamine under microwave irradiation. Adv Phys Theor Appl


Li P, Xu HB, Zhang Y, Li ZH, Zheng SL, Bai YL. The effects of Al and

Ba on the colour performance of chromic oxide green pigment. Dyes

Pigm 2009;80:287-91.

Rao TV, Yang Y, Sayari A. Ethane dehydrogenation over pore-expanded

mesoporous silica supported chromium oxide: 1. Catalysts preparation

and characterization. J Mol Catal A Chem 2009;301(1-2):152-8.

Wanga G, Zhang L, Deng J, Dai H, He H, Tong C. Preparation,

characterization and catalytic activity of chromia supported on

SBA-15 for the oxidative dehydrogenation of isobutene. Appl Catal A


Pang X, Gao K, Luo F, Emirov Y, Levin AA, Volinsky AA. Investigation

of microstructure and mechanical properties of multi-layer Cr/Cr

coatings. Thin Solid Films 2009;517(6):1922-7.

Hou X, Choy KL. Synthesis of Cr2O-based nanocomposite coatings

with incorporation of inorganic fullerene-like nanoparticles. Thin Solid

Films 2008;516(23):8620-4.3

Abecassis-Wolfovich M, Rotter H, Landau MV, Korin E, Erenburg AI, 3




How to Cite

Sangwan, P., and H. Kumar. “SYNTHESIS, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITIES OF CHROMIUM OXIDE NANOPARTICLES AGAINST KLEBSIELLA PNEUMONIAE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 2, Feb. 2017, pp. 206-9, doi:10.22159/ajpcr.2017.v10i2.15189.



Original Article(s)