• POONAM SANGWAN Department of Chemistry, GC Hisar, Haryana, India.
  • HARISH KUMAR Department of Chemistry, Central University of Mahendergarh, Haryana, India.


Objective: This study deals with the synthesis of iron oxide nanoparticles by sol-gel technique, their characterization and antibacterial activity of these nanoparticles against Staphylococcus epidermidis.

Methods: Hematite (α-Fe2O3) nanoparticles were successfully synthesized by sol-gel method using tetraethyl orthosilicate as a precursor. The structural morphology, size, and chemical state of synthesized iron oxide nanoparticles have been investigated by X-ray diffractometer (XRD), transmission electron microscopy, Fourier transform infrared spectroscopy, and ultraviolet-visible spectroscopy. The antibacterial activities of these iron oxide nanoparticles were investigated on a pathogenic bacteria S. epidermidis, by measuring the zone of inhibition and colony-forming units on solid medium and by measuring the optical density of the culture solution. Antibacterial activity of iron oxide nanoparticles was also compared with well-known standard antibiotics.

Results: It was confirmed from XRD data that the synthesized iron oxide nanoparticles were hematite (α-Fe2O3) nanoparticles. Average particle size of the Fe2O3 nanoparticles was found to be 38.57 nm by XRD characterization. The antibacterial activity of Fe2O3 nanoparticles was almost comparable to the most of the standard antibiotics (taken for comparison), but Fe2O3 nanoparticles were found to be more effective than antibiotic ampicillin and sulfatriad toward S. epidermidis.

Conclusion: This study shows that Fe2O3 nanoparticles possess good antibacterial properties; therefore, these metal nanoparticles may be used in place of antibiotics. These inorganic metal nanoparticles can be used by pharmaceutical industries for further research regarding the toxicity study for its use in human being.

Keywords: Fe2O3, Sol-Gel, X-ray diffractometer, Transmission electron microscopy


1. Lee C, Kim JY, Lee WI, Nelson KL, Yoon J, Sedlak DL. Bactericidal effect of zero-valent iron nanoparticles on Escherichia coli. Environ Sci Technol 2008;42:4927-33.
2. Hasanzadeh M, Shadjou N, Guardia ML. Iron and iron-oxide magnetic nanoparticles as signal-amplification elements in electrochemical biosensing. Trends Analyt Chem 2015;72:1-9.
3. George JM, Antony A, Mathew B. Metal oxide nanoparticles in electrochemical sensing and biosensing. Mikrochim Acta 2018;185:358.
4. Behera SS, Patra JK, Pramanik K, Panda N, Thatoi H. Characterization and evaluation of antibacterial activities of chemically synthesized iron oxide nanoparticles. World J Nano Sci Eng 2012;2:196-200.
5. Beets-Tan RG, Van Engelshoven JM, Greve JW. Hepatic adenoma and focal nodular hyperplasia: MR findings with superparamagnetic iron oxide-enhanced MRI. Clin Imaging 1998;22:211-5.
6. Martinkova P, Brtnicky M, Kynicky J, Pohanka M. Iron oxide nanoparticles: Innovative tool in cancer diagnosis and therapy. Adv Healthc Mater 2017;7:1-14.
7. Saeed M, Ren W, Wu A. Therapeutic applications of iron oxide based nanoparticles in cancer: Basic concepts and recent advances. Biomater Sci 2018;6:708-25.
8. Gupta AK, Gupta M. Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 2005;26:3995-4021.
9. Lida H, Takayanagi K, Nakanishi T, Osaka T. Synthesis of Fe3O4 nanoparticles with various sizes and magnetic properties by controlled hydrolysis. J Colloid Interface Sci 2007;314:274-80.
10. Lewandowska J, Staszewska M, Kepczynski M, Szuwarzynski M, Latkiewicz A, Olejniczak Z, et al. Sol-gel synthesis of iron oxide-silica composite microstructures. J Solgel Sci Technol 2012;64:67-77.
11. Reda SM. Synthesis of ZnO and Fe2O3 nanoparticles by sol-gel method and their application in dye-sensitized solar cell. Mater Sci Semicond Process 2010;13:417-25.
12. Ma J, Lian J, Duan X, Liu X, Zheng W. ?-Fe2o3: Hydrothermal synthesis, magnetic and electrochemical properties. J Phys Chem C 2010;114:10671-6.
13. Al-Alawy AF, Al-Abodi E, Kadhim RM. Synthesis and characterization of magnetic iron oxide nanoparticles by co-precipitation method at different conditions. Univ Baghdad Eng J 2018;24:60-72.
14. Kanda WK, Horwongsakul S. The preparation of iron (III) oxide nanoparticles using W/O microemulsion. Mater Lett 2011;65:2820-2.
15. Sangwan P, Kumar H, Rani R. Wet chemical synthesis, characterization, and antibacterial activity of molybdenum oxide nanoparticles against Staphylococcus epidermidis and Enterobacter aerogenes. Asian J Pharm Clin Res 2019;12:59-63.
16. Sangwan P, Kumar H. Synthesis, characterization and antibacterial activities of chromium oxide nanoparticles against Klebsiella pneumoniae. Asian J Pharm Clin Res 2017;10:1-4.
17. Mohammadi SZ, Motlagh MK, Jahani S, Yousef M. Synthesis and characterization of ?-Fe2O3 nanoparticles by microwave method. Int J Nanosci Nanotechnol 2012;8:87-92.
18. Bagheri S, Chandrappa KG, Hamid SB. Generation of hematite nanoparticles via sol-gel method. Res J Chem Sci 2013;3:62-8.
19. Zhao B, Wang Y, Guo H, Wang J, He Y, Jiao Z, et al. Iron oxide (III) nanoparticles fabricated by electron beam irradiation method. Mater Sci Pol 2007;25:1143-8.
20. Sahoo SK, Agarwal K, Singh AK, Polke BG, Raha KC. Characterization of ?- and ?-Fe2O3 Nano powders synthesized by emulsion precipitation-calcination route and rheological behaviour of ?-Fe2O3. Int J Eng Sci Technol 2010;2:118-26.
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How to Cite
SANGWAN, P., and H. KUMAR. “SYNTHESIS CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY OF IRON OXIDE NANOPARTICLES AGAINST STAPHYLOCOCCUS EPIDERMIDIS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 9, June 2020, pp. 49-52, doi:10.22159/ajpcr.2020.v13i9.36938.
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