CO-INFECTION OF BRUCELLA AND DENGUE VIRUS
Objective: Brucellosis is an important zoonotic infection having a worldwide distribution. In South East Asian developing countries like India, brucellosis is yet well-known but often neglected disease. Although dengue fever has a global distribution, South East Asian region together with the Western Pacific region represents 75% of the current global disease burden. Frequent dengue fever outbreaks have been encountered in different parts of India. Both brucellosis and dengue fever may present with many nonspecific symptoms. This study was designed to identify the trend of their co-infection.
Methods: The study was carried out from February 2015 to July 2016 in Eastern India. Blood samples collected from clinically suspected dengue fever cases were confirmed serologically by dengue specific NS1, IgM, and IgG capture ELISA methods. The confirmed dengue samples were further subjected to Brucella specific serological and molecular investigations.
Results: Out of 37 serologically confirmed dengue cases, 8 (21.6%) showed co-infection of brucellosis and dengue fever. Female predominance was observed among the co-infection cases. Most of these cases belonged to the age group 21-40 years and resided in and around Kolkata.
Conclusion: This study portrays the simultaneous acquisition of these two pathogens in a few patients. Co-infection of these two pathogens, while feasible, has not been accounted previously in this part of India. Thus, this report highlights the significance of studying the possibility of occurrence of dual infection of these two diseases and its outcome in patients with febrile illness thereby providing future scope of preventing case fatalities
Keywords: Brucellosis, Dengue, Co-infection, ELISA, Polymerase chain reaction.
2. Saha S, Gupta D, Das S. Autoimmune changes in human brucellosis. Int J Biopharm 2013;4:131-4.
3. Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. N Engl J Med 2005;352(22):2325-36.
4. Chatterjee D, Halder D, Chakraborty U, Dutta D, Kuila P, Das S. Antimicrobial activities of pharmacological and antioxidant compounds of some edible mushrooms against Brucella spp. Int J Pharm Sci Res 2016. [In Press].
5. Sen A, Banerjee P, Dutta D, Pal MK, Ray A, Das S. Brucellar uveitis in Eastern India. Asian J Pharm Clin Res 2016. [In Press].
6. Atluri VL, Xavica MN, De Jong MF, Den Hartigh AB, Tsolis RE. Interactions of the human pathogenic Brucella species with their hosts. Annu Rev Microbiol 2011;65:523-41.
7. Lambrechts L, Scott TW, Gubler DJ. Consequences of the expanding global distribution of Aedes albopictus for dengue virus transmission. PLoS Negl Trop Dis 2010;4(5):e646.
8. Garg A, Garg J, Rao YK, Upadhay GC, Sakhuja S. Prevalence of dengue among clinically suspected febrile episodes at a teaching hospital in North India. J Infect Dis Immun 2011;3:85-9.
9. Ayyub M, Al-Juhani NR, Alfi AY, Al-Ukayli S. Brucellosis and dengue fever â€“ a co-infection or cross reactivity? Biomedica 2006;22:80-3.
10. Bzeizi KI, Benmousa A, Sanai FM. Coincidence of acute Brucella hepatitis and dengue fever or serologic cross-reactivity? Saudi J Gastroenterol 2010;16(4):299-301.
11. Vijayakumar TS, Chandy S, Sathish N, Abraham M, Abraham P, Sridharan G. Is dengue emerging as a major public health problem? Indian J Med Res 2005;121(2):100-7.
12. Innis BL, Nisalak A, Nimmannitya S, Kusalerdchariya S, Chongswasdi V, Suntayakorn S, et al. An enzyme-linked immunosorbent assay to characterize dengue infections where dengue and Japanese encephalitis co-circulate. Am J Trop Med Hyg 1989;40(4):418-27.
13. Laboratory Guidance and Diagnostic Testing. Centers for Disease Control and Prevention. Available from: http://www.cdc.gov/dengue/ clinicallab/laboratory.html. [Last accessed on 2013 Jul 20].
14. Chart H, Okubadejo OA, Rowe B. The serological relationship between Escherichia coli O157 and Yersinia enterocolitica O9 using sera from patients with brucellosis. Epidemiol Infect 1992;108(1):77-85.
15. Makino Y, Tadano M, Saito M, Maneekarn N, Sittisombut N, Sirisanthana V, et al. Studies on serological cross-reaction in sequential Flavivirus infections. Microbiol Immunol 1994;38(12):951-5.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.