• Prabir Kuila
  • Devarati Dutta
  • Urmita Chakrabarty
  • Debasmita Chatterjee
  • Satadal Das Brucella Research Laboratory, Peerless Hospital & B. K. Roy Research Centre, Kolkata


Objective: Brucellosis is an important zoonotic disease throughout the globe and other developing countries. The present study was aimed to compare results of different serological tests and PCR for diagnosis of brucellosis in patients sufferings from fever in Kolkata and in adjoining districts.

Methods: A total of 2088 serum samples were collected from the patients suffering from fever from January, 2013 to September, 2015. The samples were tested by serological tests STAT, RBPT, ELISA(IgM,IgG) and Brucella genus specific PCR.

Results: The study revealed decreasing positive results by STAT (18.43%, N=385), RBPT (12.59%, N=263), IgM ELISA (7.71%, N=161), PCR (4.21%, N=88) and IgG ELISA (1.43%, N=30). When serological tests were compared with PCR, it was found that both STAT and PCR were positive in 84 samples( 4.02%), both RBPT and PCR were positive in 65 samples(3.11%), both IgM and PCR were positive in 51 samples(2.44%) and both IgG and PCR were positive in 9 samples(0.43%).

Conclusion: In this cross sectional study in a zonal population of India it was found that STAT was the most sensitive test for diagnosis of brucellosis followed by RBPT when compared to PCR test results. Four STAT negative samples showed positive results in PCR, which were positive by RBPT test. This indicates that if we combine STAT and RBPT for diagnosis of brucellosis then both sensitivity and specificity of the combined test will increase.


Keywords: Brucellosis, STAT, RBPT, ELISA, Genus specific PCR.

Author Biography

Satadal Das, Brucella Research Laboratory, Peerless Hospital & B. K. Roy Research Centre, Kolkata

Principal Investigator,



1) Sathyanarayan MS, Suresh D, Suresh BS, Krishna S, Mariraj J, Surekha Sa, Ravichandra P, Ravikumar R.A Comparative study of agglutination tests,blood culture and ELISA in the laboratory diagnosis of human brucellosis. Int J Biol Med Res 2011;2(2):569-572

2) Saha S, Gupta D, Das S. Autooimmuno changes in human brucellosis. International Journal of Biopharmaceutics 2013; 4(2):131-134.

3) Prakash P, Bhansali S,Gupta E,Kothari D, Mathur A, Ambuwani S, Epidemiology Of Brucellosis In High Risk Group & PUO Patients Of Western-Rajasthan. Natl J Commun Med 2012 ;3(1):61-65.

4) Smits LH, Kadri M. Brucellosis in India: a deceptive infectious disease. Indian J Med Res 2005;122:375-384
5) Hassanain A N, Ahmed M W. Efficiency of serological tests in comparison with PCR for Diagnosis of brucellosis. World J of Med Sc 2012 7(4):243-247.

6) Mangalgi SS, Sajjan GA, Mohite T S. Brucellosis: A cause for pyrexia of unknown origin. Int J Biol Med Res 2012;3(3):2054-2055.

7) Ghatak S, Muthukumaran B R, and Nachimuthu K S.A simple method of genomic DNA extraction from human samples for PCR-RELP analysis. J Biomol Tech 2013;24:224-231.

8) Joshi M, Deshpande J.D, Polymerase chain reaction: methods,principles and application. Int J Biomed Res 2010 1[5]81-97.

9) Akobeng K A. Understanding diagnosis tests 1:sensitivity,specificity and predictive values. Acta Paediatr 2006;96:338-341

10) Matovic K, Asanin R, Radojicic S, Lako B, Zarkovic A. Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis. Acta Veterinaria (Beograd) 2008;58:467-476.

11) Yohannes M, Gill JPS, Ghatak S,Sing D K, Tolosa T. Comparative evaluation of the rose bengal plate test ,standard tube agglutination test and complement fixation test for the diagnosis of human brucellosis. Rev sci tech off int Epiz 2012;31(3):979-984

12) Metri B C, Baragundi M C, Jyothi P, Lava R, Basavarajappa, Hanumanthapa A R, Chandrappa N R. Seroprevelance of brucellosis in Davangere,Karnataka.J Clin Diagn Res 2011; 5(1):41-44
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How to Cite
Kuila, P., D. Dutta, U. Chakrabarty, D. Chatterjee, and S. Das. “COMPARISON OF SEROLOGICAL TESTS AND PCR FOR DIAGNOSIS OF HUMAN BRUCELLOSIS SUFFERING FROM FEVER”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 5, May 2017, pp. 109-11, doi:10.22159/ajpcr.2017.v10i5.17016.
Original Article(s)