BACTERIOLOGICAL PROFILE AND ANTIMICROBIAL RESISTANCE PATTERNS ISOLATES IN PUS SAMPLES AT AGARTALA GOVERNMENT MEDICAL COLLEGE
Objective: Injudicious use of antibiotics in the management of pyogenic infections leads to emergence and spread of antibiotic resistance among pyogenic bacteria. This study aimed toward the determination of the bacterial isolates from pus samples and their antibiotic resistance pattern.
Methods: A retrospective analysis of 359 consecutive pus specimens received at Microbiology Department of Gobind Ballabh Pant Hospital under
Agartala Government Medical College, Agartala has been done. Bacterial isolates were identified by standard microbial techniques, and antibiotic susceptibility was done by modified Kirby-Bauer methods.
Results: Growth was seen in 176 (49.02%) specimens out of 359 samples. A total of 176 specimens yielded single isolate whereas 4 specimens yielded 2 isolates. Staphylococcus aureus was the most common organism isolated 53 (30.11%) followed by Pseudomonas spp. 37 (21.02%), Klebsiella spp. 30 (17.07%), Escherichia coli 24 (13.63%) Proteus spp. 11 (6.40%), Acinetobacter spp. 7 (3.97%), Citrobacter spp., and Enterobacter spp. 4 (2.27%) each. The highest number of multidrugs resistant isolates was Klebsiella spp. All S. aureus were 100% sensitive to vancomycin, and all Gram-negative bacilli were 100% to sensitive to imipenem and amikacin.
Conclusion: This study revealed the most common organism in pus samples is S. aureus followed by Pseudomonas spp. and highly multidrug resistance Klebsiella spp. Hence, continued monitoring of susceptibility pattern need to be carried out to detect the true burden of antibiotic resistance in organism and prevent their further emergence by judicious use of drugs.
Keywords: Pyogenic bacteria, Antibiotic resistance, Multidrug-resistant Klebsiella, Antibiotic policy.
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