THE ANTHELMINTIC EFFECTS OF ETHANOL EXTRACT OF CURANGA FEL-TERRAE LEAVES ON ASCARIDIA GALLI

  • Popi Patilaya Universitas Sumatera Utara
  • Dadang Irfan Husori Universitas Sumatera Utara
  • Imam Bagus Sumantri Universitas Sumatera Utara

Abstract

ABSTRACT
Objective: This study was to investigate the anthelmintic effects of ethanol extract of Curanga fel-terrae leaves on Ascaridia galli.
Methods: Extract of C. fel-terrae was prepared by percolating the dried powder of the plant leaves. To observe anthelmintic effects, A. galli was
exposed to the plant extract solutions in different concentrations at room temperature for 72 hrs. Sodium chloride 0.9%, sodium carboxymethyl
cellulose 0.5%, and albendazole 0.1% were served as negative control, solvent control, and positive control, respectively. The anthelmintic effects
were determined by observing the time taken for paralysis and the time taken for death of A. galli during experiment.
Results: The results demonstrated that there were no different effects between negative and solvent controls. However, the ethanol extract of
C. fel-terrae leaves exhibited significantly paralysis and death effects toward A. galli. The plant extracts at the concentration of 100 mg/ml showed
similar anthelmintic effects with albendazole 0.1%. This study also indicated that the paralysis and death effects of plant extract were shorter when
the worms exposed with the concentration above of 100 mg/ml.
Conclusion: This study indicated that the ethanol extract of C. fel-terrae leaves has potential anthelmintic effects on A. galli.
Keywords: Curanga fel-terrae, Ascaridia galli, Anthelmintic, Helminthiasis, Ascariasis.

 

References

REFERENCES
1. World Health Organization. Water sanitation hygiene: Water related
diseases: Ascariasis; 2001. Available from: http://www.who.int/
water_sanitation_health/diseases/ascariasis/en/. [Last accessed on
2016 Aug 17].
2. Indonesian Ministry of Health. Guidelines of Clinical Practices for
Physician in Primary Health Care Facility. Jakarta: Indonesian Ministry
of Health; 2014.
3. Arizono N, Yoshimura Y, Tohzaka N, Yamada M, Tegoshi T, Onishi K,
et al. Ascariasis in Japan: Is pig-derived Ascaris infecting humans? JpnJ Infect Dis 2010;63(6):447-8.
4. Tagboto S, Townson S. Antiparasitic properties of medicinal plants and
other naturally occurring products. Adv Parasitol 2001;50:199-295.
5. van Balgooy MM. An update survey of Malesian seeds plants families.
Reinwardtia 2010;13(2):171-81.
6. Rahmanzadeh R, Müller K, Fischer E, Bartels D, Borsch T. The
linderniaceae and gratiolaceae are further lineages distinct from the
scrophulariaceae (Lamiales). Plant Biol (Stuttg) 2005;7(1):67-78.
7. Tinton A. Smart Book of Medicinal Plants. 1st ed. Jakarta: Agromedia
Pustaka; 2008.
8. Patilaya P, Husori DI. Preliminary study on the anthelmintic activity
of the leaf ethanolic extract of Indonesian Curanga fel-terrae (Lour.)
Merr. Int J Pharm Technol Res 2015;8(3):347-51.
9. Rodriguez JY, Craig TM. Parasites of Backyard Chickens. Clinician’s
Brief; 2015. p. 65-9. Available from: http://www.cliniciansbrief.com/
article/parasites-backyard-chickens. [Last accessed on 2016 Oct 13].
10. Rahemo ZI, Hussain SS. Histological study on the body wall of
Ascaridia galli (Nematoda). J Entomol Nematol 2009;1(6):74-82.
11. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening
and extraction: A review. Int Pharm Sci 2011;1(1):98-106.
12. Huang Y, De Bruyne T, Apers S, Ma Y, Claeys M, Pieters L, et al.
Flavonoid glucuronides from Picria fel-terrae. Phytochemistry
1999;52(8):1701-3.
13. Zou JM, Wang LS, Ma XM, Shi RB, Guo YJ. Isolation and identification
of a new cucurbitacin from Picria fel-terrae. Yao Xue Xue Bao
2004;39:910-2.
14. Huang Y, De Bruyne T, Apers S, Ma Y, Claeys M, Vanden Berghe D,
et al. Complement-inhibiting cucurbitacin glycosides from Picria
fel-terrae. J Nat Prod 1998;61(6):757-61.
15. Fang H, Ning DS, Liang XY. Studies on technology optimization for
extracting triterpenoid saponins from Picria fel-terrae by multi-target
grading method. J Chin Med Mater 2009;32(12):1902-5.
16. Sitorus P, Harahap U, Pandapotan M, Barus T. Isolation of β-sitosterol
from n-hexane extract of Picria fel-terrae Lour. Leave and study of
its antidiabetic effect in alloxan induced diabetic mice. Int J Pharm
Technol Res 2014;6(1):137-41.
17. Kumarasingha R, Karpe AV, Preston S, Yeo TC, Lim DS, Tu CL,
et al. Metabolic profiling and in vitro assessment of anthelmintic
fractions of Picria fel-terrae Lour. Int J Parasitol Drugs Drug Resist
2016;6(6):171-8.
18. Neves BJ, Andrade CH, Cravo PV. Natural products as leads in
schistosome drug discovery. Molecules 2015;20(2):1872-903.
19. Enejoh OS, Suleiman MM, Ajanusi JO, Ambali SF. In vitro anthelmintic
efficacy of extracts of Citrus aurantifolia (Christm) swingle fruit peels
against Heligmosomoides bakeri ova and larvae. Int J Curr Pharm Res
2015;7(2):92-6.
20. Bi S, Goyal PK. Anthelmintic effect of natural plant (Carica papaya)
extract against the gastrointestinal nematode, Ancylostoma caninum in
mice. ISCA J Biol Sci 2012;1(1):2-6.
21. Subash KR, Rao NJ, Cheriyan BV, Bhaarati GM, Kumar KS. The
anthelmintic activity of Eupatorium triplinerve and Alpinia galanga in
Pheretima posthuma and Ascaridia galli: A comparative study. J Clin
Diagn Res 2012;6(6):947-50.
22. Jiju V, Gorantla M, Chamundeeswari D. Evaluation of anthelmintic
activity of methanolic extract of Asystasia gangeticum. Int J Pharm Life
Sci 2013;4(6):2727-30.
23. Husori DI, Bancin DY, Muhaimin, Bahri S, Patilaya P. Anthelmintic
activity of ethanolic and aqueous extracts of Allium fistulosum L. Leaves
on Ascaris lumbricoides. Int J Pharm Clin Res 2016;8(9):1310-3.
24. Kumar R, Solanki R, Triphati L. In vitro anthelmintic activity of seeds
of Cicer arietinum L. Asian. J Chem 2013;25(9):5109-10.
25. Kour M, Singh H, Kaur J. Anthelmintic activity of hydroalcoholic
extract of leaves of Valeriana jatamansi. World J Pharm Sci
2014;2(12):1741-5.
26. Olusegun-Joseph TS, Ofodile LN, Oguntoke T. In vitro evaluation of
anthelmintic activity of crude extract of the leaves of Dalbergiella
welwitschii. Int J Pharm Pharm Sci 2013;5 Suppl 1:32-3.
27. Paria S, Maity S, Mookerjee M. Phytochemial investigation and
evaluation of anthelmintic activities of V. Negundo leaf extract. Int J
Res Pharm Biomed Sci 2012;3:1143-6.
28. Joshi N, Bhatt S, Dhyani S, Nain J. Phytochemical screening of
secondary metabolites of Argemone mexicana linn flowers. Int J Curr
Pharm Res 2013;5:144-7.
29. Goel AK, Kulshreshtha DK, Dubey MP, Rajendran SM. Screening
of Indian plants for biological activity: Part XVI. Indian J Exp Biol
2002;40(7):812-27.
30. Azaizeh H, Halahleh F, Abbas N, Markovics A, Muklada H, Ungar ED,
et al. Polyphenols from Pistacia lentiscus and Phillyrea latifolia impair
the exsheathment of gastro-intestinal nematode larvae. Vet Parasitol
2013;191(1-2):44-50.
31. Kumar BS, Lakshman K, Jayaveera KN, Velmurugan C, Manoj B,
Sridhar SM. Anthelmintic activity of methanol extract of Amaranthus
caudatus Linn. Internet J Food Saf 2010;12:127-9.
32. Jain P, Singh S, Singh SK, Verma SK, Kharya MD, Solanki S.
Anthelmintic potential of herbal drugs. Int J Res Dev Pharm L Sci
2013;2(3):412-27.
33. Hussain A, Sonkar AK, Ahmad MP, Wahab S. In vitro anthelmintic
activity of Coleus aromaticus root in Indian adulth earthworm. Asian
Pac J Trop Dis 2012;2 Suppl 1:425-7.
34. Wang GX, Han J, Zhao LW, Jiang DX, Liu YT, Liu XL. Anthelmintic
activity of steroidal saponins from Paris polyphylla. Phytomedicine
2010;17(14):1102-5.
35. Borba HR, Freire RB, Albuquerque AC, Cardoso ME, Braga IG,
Almeida ST, et al. Anthelmintic comparative study of Solanum
lycocarpum St. Hill extracts in mice naturally infected with Aspiculuris
tetraptera. Nat Sci 2010;8(4):94-100.
36. Gaikwad SS, Kale AA, Jadhav BG, Deshpande NR, Salvekar JP.
Anthelmintic activity of Cassia auriculata L. Extracts-In vitro study.
J Nat Prod Plant Resour 2011;1(2):62-6.
37. Chandrashekhar CH, Latha KP, Vagdevi HM, Vaidya VP. Anthelmintic
activity of the crudeextracts of Ficus racemosa. Int J Green Pharm
2008;2(2):100-3.
38. Kundu S, Roy S, Nandi S, Ukil B, Lyndem LM. In vitro anthelmintic
effects of Senna occidentalis (L.) Link (Leguminosae) on rat tapeworm
Hymenolepis diminuta. Int J Pharm Pharm Sci 2015;7(6):268-72.
39. Swargiary A, Roy B. In vitro anthelmintic afficacy of Alpinia nigra
and its bioactive compound, astragalin against Fasciolopsis buski. Int J
Pharm Pharm Sci 2015;7(10):30-5.
Statistics
444 Views | 416 Downloads
Citatons
How to Cite
Patilaya, P., D. I. Husori, and I. B. Sumantri. “THE ANTHELMINTIC EFFECTS OF ETHANOL EXTRACT OF CURANGA FEL-TERRAE LEAVES ON ASCARIDIA GALLI”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 3, Mar. 2017, pp. 117-9, doi:10.22159/ajpcr.2017.v10i3.15504.
Section
Original Article(s)