ANTIMUTAGENIC POTENTIAL OF LIV52 BY AMES SALMONELLA/MAMMALIAN: MICROSOME MUTAGENICITY ASSAY


Radhakrishna Manipura, Hedge Mj

Abstract


ABSTRACT
Objective: Liv52, a nontoxic herbal preparation is reported to be clinically active in hepatotoxicity and a wide range of hepatic disorders. This study
was undertaken to evaluate the mutagenic/antimutagenic potential of Liv52 using Salmonella mutagenicity test.
Methods: Ames Salmonella/Mammalian - microsome mutagenicity test was used to evaluate the mutagenic or antimutagenic potential of Liv52.
Salmonella typhimurium tester strains TA1537, TA1538, TA100, and TA102 were used for mutagenicity testing. The antimutagenicity study was
carried out in tester strains TA1538 and TA100 against various standard mutagens with and without metabolic activation.
Results: Liv52 did not show any mutagenic potential both with and without metabolic activation, whereas in TA1538 and TA 100 tester strains, Liv52
showed 48.4% and 47.2% of inhibition of his+ revertants induced by 4-nitro-O-phenylenediamine, respectively. Further with metabolic activation
in TA1538, Liv52 showed 99.8%, 99.8%, and 100% inhibition of his+ revertants induced by 2-aminofluorene, 2-anthranine, and cigarette smoke
condensate, respectively. In TA100 maximum of 100%, 100%, 97.7%, and 100% inhibition of his+ revertants induced by 2-aminofluorene, 2-anthranine,
benzo(a)pyrene, and cigarette smoke condensate, respectively, were observed. A significant enhancement of inhibition of his+ revertants induced by
all the above said mutagens were observed in preincubation modification method.
Conclusion: Liv52 was found to be nonmutagenic in Salmonella assay, whereas manifested the antimutagenic potential both with and without
metabolic activation. The enhanced antimutagenic activity of Liv52 on preincubation indicates that the antimutagenic factor(s) may be desmutagenic
in nature. The exact mechanism by which Liv52 exerts antimutagenic potential is not known. The possibility of having diverse antimutagenic factors
in Liv52 which act by different mechanisms are strongly indicated.
Keywords: Liv52 syrup, Salmonella mutagenicity test, Ames test, Desmutagens.


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About this article

Title

ANTIMUTAGENIC POTENTIAL OF LIV52 BY AMES SALMONELLA/MAMMALIAN: MICROSOME MUTAGENICITY ASSAY

DOI

10.22159/ajpcr.2017.v10i3.16148

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 277-282

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

85 Views | 56 Downloads

Authors & Affiliations

Radhakrishna Manipura
Manipal University
India

Hedge Mj


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