IS ACQUISITION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS - AN OCCUPATIONAL HAZARD FOR MEDICAL STUDENTS IN INDIA?


Shreyas K, Radhakrishna M, Ashwini Hegde, Pooja Rao

Abstract


 

 Objective: The incidence of community-acquired and hospital-acquired Staphylococcus aureus infections has been rising with increasing emergence of drug-resistant strains called methicillin-resistant S. aureus (MRSA). The main objective of this study was to determine the prevalence of carriage rate of MRSA among the interns of tertiary care teaching hospital in Mangalore.

Methods: A total of 150 interns were participated, thereby swabs from both anterior nares were collected and processed for S. aureus. The isolated strains of S. aureus were screened for methicillin susceptibility by modified Kirby–Bauer method using cefoxitin (30 μg) disc. Further, antibiotic susceptibility testing for all isolates of S. aureus was also done against selected appropriate antibiotics. Vancomycin susceptibility testing for MRSA was done by E test.

Results: The number of strains of S. aureus isolated from our 150 participants was 71 with a percentage rate of 47.3. Of the 71 isolates of S. aureus, one (1.4%) was MRSA. The overall MRSA carriage rate was 0.66%. The S. aureus and MRSA carriage rates recorded in this study were at par and significantly lower, respectively, when compared with other reported studies. It was observed that risk factors such as the use of antibiotics in the past 6 months and smoking were found to be statistically significant in nasal carriage status of S. aureus (<0.05). The only one isolate of MRSA was found to be sensitive to vancomycin, linezolid, and teicoplanin.

Conclusion: It was concluded that the acquisition of S. aureus and MRSA would not be an occupational hazard for medical students of developing countries like India.


Keywords


Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Nasal carriage, Medical interns.

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References


Foster TJ. The Staphylococcus aureus ‘superbug’. J Clin Invest 2004;114(12):1693-6.

Peacock SJ, de Silva I, Lowy FD. What determines nasal carriage of Staphylococcus aureus? Trends Microbiol 2001;9(12):605-10.

Deresinski S. Methicillin-resistant Staphylococcus aureus: An evolutionary, epidemiologic, and therapeutic odyssey. Clin Infect Dis 2005;40(4):562-73.

Chambers HF. The changing epidemiology of Staphylococcus aureus? Emerg Infect Dis 2001;7(2):178-82.

Salgado CD, Farr BM, Calfee DP. Community-acquired methicillin-resistant Staphylococcus aureus: A meta-analysis of prevalence and risk factors. Clin Infect Dis 2003;36(2):131-9.

Bischoff WE, Wallis ML, Tucker KB, Reboussin BA, Sherertz RJ. Staphylococcus aureus nasal carriage in a student community: Prevalence, clonal relationships, and risk factors. Infect Control Hosp Epidemiol 2004;25(6):485-91.

Albrich WC, Harbarth S. Health-care workers: Source, vector, or victim of MRSA? Lancet Infect Dis 2008;8(5):289-301.

Eveillard M, Martin Y, Hidri N, Boussougant Y, Joly-Guillou ML. Carriage of methicillin-resistant Staphylococcus aureus among hospital employees: Prevalence, duration, and transmission to households. Infect Control Hosp Epidemiol 2004;25(2):114-20.

Rohde RE, Denham R, Brannon A. Methicillin resistant Staphylococcus aureus: Carriage rates and characterization of students in a Texas university. Clin Lab Sci 2009;22(3):176-84.

Cheesbrough M. District Laboratory Practice in Tropical Countries. Part 2. Cambridge: Cambridge University Press; 2008.

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45(4):493-6.

Clinical and Laboratory Standards Institute. Surveillance for MRSA: Principles, Practices, and Challenges; A Report. CLSI Document X07-R. Wayne, PA: Clinical and Laboratory Standards Institute; 2010.

Clinical and Laboratory Standards Institute. NCCLS Performance Standards for Antimicrobial Disc Diffusion Tests; Approved Standards, CLSI Document M2-M9. 9th ed. Wayne, PA: Clinical and Laboratory Standards Institute; 2006.

Peacock SJ, Justice A, Griffiths D, de Silva GD, Kantzanou MN, Crook D, et al. Determinants of acquisition and carriage of Staphylococcus aureus in infancy. J Clin Microbiol 2003;41(12):5718-25.

Kluytmans J, van Belkum A, Verbrugh H. Nasal carriage of Staphylococcus aureus: Epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev 1997;10(3):505-20.

Robicsek A, Beaumont JL, Peterson LR. Duration of colonization with methicillin resistant Staphylococcus aureus. Clin Infect Dis 2009;48(7):910-3.

Bellows C, Smith A, Wheeler J, Morici L. Nasal carriage of methicillin-resistant Staphylococcus aureus among students at a Louisiana medical university. Braz J Infect Dis 2013;17(1):118-9.

Amiry AA. Methicillin resistant Staphylococcus aureus: An occupational health hazard in the prehospital setting. J Acute Dis 2015;4(7):274-6.

Susethira AR, Saiprasanna N, Vigenshwari RS, Uma A, Prabhu N, subramanian TK. Methicillin resistant Staphylococcus aureus-nasal carriage among healthcare workers. J Glob Biosci 2015;4(4):2518-24.

Radhakrishna M, Taneja A, Rao P. Nasal carriage of Staphylococcus aureus with special emphasis on methicillin resistant Staphylococcus aureus among students of a south Indian medical college-prevalence and antibiogram pattern. Asian J Pharm Clin Res 2016;9 Suppl 2:129-32.

Santhosh DV, Shobha KL, Bairy I, Rao G, Anand KM, D’Souza J. Nasal screening and survey of pre-clinical medical students from Malaysia for nasal carriage of coagulase positive MRSA and rate of nasal colonization with Staphylococcus species. J Clin Diagn Res 2007;1(6):494-9.

Treesirichod A, Hantagool S, Prommalikit O. Nasal carriage and antimicrobial susceptibility of Staphylococcus aureus among medical students at the HRH Princess Maha Chakri Sirindhorn Medical Center, Thailand: A cross sectional study. J Infect Public Health 2013;6(3):196-201.

Ma XX, Sun DD, Wang S, Wang ML, Li M, Shang H, et al. Nasal carriage of methicillin-resistant Staphylococcus aureus among preclinical medical students: Epidemiologic and molecular characteristics of methicillin-resistant Staphylococcus aureus clones. Diagn Microbiol Infect Dis 2011;70(1):22-30.

Zakai SA. Prevalence of methicillin-resistant Staphylococcus aureus nasal colonization among medical students in Jeddah, Saudi Arabia. Saudi Med J 2015;36(7):807-12.




About this article

Title

IS ACQUISITION OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS - AN OCCUPATIONAL HAZARD FOR MEDICAL STUDENTS IN INDIA?

Keywords

Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Nasal carriage, Medical interns.

DOI

10.22159/ajpcr.2017.v10i12.20865

Date

01-12-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 12 December 2017 Page: 141-144

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

11 Views | 11 Downloads

Authors & Affiliations

Shreyas K
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
India

Radhakrishna M
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
India

Ashwini Hegde
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
India

Pooja Rao
Department of Microbiology, Kasturba Medical College, Manipal University, Mangalore, Karnataka, India.
India


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