IN VITRO IMMUNOSTIMULATION ACTIVITY OF NIGELLA SATIVA LINN. AND PSORALEA CORYLIFOLIA LINN. SEEDS USING A MURINE MACROPHAGE CELL LINE


Saravanan Periasamy, Sheik Noor Mohamed M, Jaikumar K, Marimuthu S, John Wyson W, Anand D

Abstract


ABSTRACT
Objective: The aim of this study is to analyze the phytochemical constituents and the in vitro immunomodulatory potential of ethanol extract of
Nigella sativa and Psoralea corylifolia seeds.
Methods: Phytoconstituents in the ethanol extract of N. sativa and P. corylifolia seeds were analyzed using gas chromatography and mass spectrum
and their immunomodulatory activity was investigated in vitro by cell proliferation assay with J774A.1 cell line.
Results: Thirteen biochemical constituents have been identified with ethanol extract of N. sativa and nine from the P. corylifolia. The maximum relative
activity of 138.77±0.44% cell proliferation was expressed at 25 μg/ml by N. sativa and 80.70±0.42% of proliferation by P. corylifolia at 25 μg/ml.
Conclusion: The results suggest that the ethanol extract of the plant seeds have stimulating activity on macrophage cells and could be useful for
modulating immune functions.
Keywords: Immunomodulatory, Cell proliferation, Macrophage cell line, Nigella sativa, Psoralea corylifolia.


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About this article

Title

IN VITRO IMMUNOSTIMULATION ACTIVITY OF NIGELLA SATIVA LINN. AND PSORALEA CORYLIFOLIA LINN. SEEDS USING A MURINE MACROPHAGE CELL LINE

DOI

10.22159/ajpcr.2017.v10i3.16227

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 329-332

Print ISSN

0974-2441

Online ISSN

2455-3891

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36 Views | 57 Downloads

Authors & Affiliations

Saravanan Periasamy
Assistant Professor, Department of Botany, Ramakrishna Mission Vivekananda College, Mylapore, Chennai, India
India

Sheik Noor Mohamed M

Jaikumar K

Marimuthu S

John Wyson W

Anand D

 

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