NEUROPHARMACOLOGICAL STUDY OF PHYLLANTHUS NIRURI IN SWISS ALBINO MICE
Background: Phyllanthus niruri is used throughout the world for treatment of hepatitis and kidney stones. It is used in the Indian Ayurvedic system of medicine in problems of gastrointestinal system, genitourinary system and kidney. To know behavioral, neurological, autonomic changes after administration of test drug in rodents, Irwin suggested neuropharmacological profile. This test is used for assessing safety pharmacology in rodents.
Methods: A total of 24 mice was divided into 4 groups of 6 each (3-male,3-female in each group). Aqueous extract of Phyllanthus niruri was prepared. Based on body weight aqueous extract was given to the mice by orally through gavage tube (Group I -300mg/kg, Group II-600mg/kg, Group III-900mg/kg, Group IV-1200mg/kg). Neuropharmacological profile is studied for each mice using Irwin’s observational test, the mice were observed for 4h after oral administration for various behavioral, neurological and autonomic changes at 0,1,2,3,4 hr.
Results: P.niruri showed negligible actions at 300 mg/kg and 600mg/kg body weight. At 900mg/kg and 1200mg/kg P.niruri showed certain behavioral and neuronal changes. P.niruri increased alertness, stereotypy, restlessness, irritability/aggressiveness in behavioral profile indicating that the drug is a CNS stimulant. Also it showed mild tremors in neurological profile indicating CNS excitation.
Conclusion: Aqueous extract of Phyllanthus niruri at 900mg/kg and 1200mg/kg showed changes in behavioral profile, neurological profile, showing it as CNS stimulant properties. Since it is an observational study further research should be done to explore CNS stimulant properties in various in-vivo studies.
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