PRODUCTION OF KERATINASES FROM NOCARDIOPSIS SP. 28ROR AS A NOVEL IRAQI STRAIN


Rabab Omran Al-jelawi-

Abstract


Objectives: isolate a novel feather- degrading actinobacterial species had the ability to produce wide pH activity keratinases.

Methods: Of 23 actinobacterial isolates were recovered from farm soil, poultry farm soil and feather wastes, these isolates were screened for protease and keratinase production on skim milk agar, feather  media, and  antimicrobial production. One potential  isolate was identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene analysis and optimized keratinase production. 

Results:   11 isolates out of 22 protease producer  had the ability to degrade raw chicken feather and some of these  isolates produced  antifungal and antibacterial metabolites.The potential isolate,  Nocardiopsis sp. 28ROR (GenBank: KC702802.1), produced two types of extracellular keratinases in feather meal medium at pH6 (acid type), 30-35°C  for 7d  and  the alkaline keratinase at pH10, 40°C  for 7d.

Conclusion: The Nocardiopsis sp. 28ROR was a novel strain produced keratinases using feather meal degradation as a cheap waste medium. The wide tolerance of temperature and pH by keratinase makes it an ideal contender to be investigated further for potential application as a detergent additive.

Keywords: Nocardiopsis, Keratinase, Optimization, Feather medium, Antibiotic.

 


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About this article

Title

PRODUCTION OF KERATINASES FROM NOCARDIOPSIS SP. 28ROR AS A NOVEL IRAQI STRAIN

DOI

10.22159/ajpcr.2017.v10i4.16427

Date

01-04-2017

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Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 4 April 2017 Page: 160-168

Print ISSN

0974-2441

Online ISSN

2455-3891

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15 Views | 30 Downloads

Authors & Affiliations

Rabab Omran Al-jelawi-
babylon university
Iraq

 

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